Illumina Complete Long Reads with Enrichment, Human

A cost-effective, flexible, targeted long-read solution to address challenging-to-map regions of the human genome. Read More...
Select Product(s)
What products do I need?

Illumina Complete Long Read Prep with Enrichment, Human (24 samples)

20113832

Illumina Complete Long Read Prep with Enrichment, Human (96 samples)

20113833

Illumina Complete Long Read Prep with Enrichment, Human Comprehensive Panel (24 samples)

20113834

Illumina Complete Long Read Prep with Enrichment, Human Comprehensive Panel (96 samples)

20113835

Illumina Complete Long Read Prep with Enrichment, HCP-24, S4 Starter Pack

20113838

Illumina Complete Long Read Prep with Enrichment, HCP-24, 10B Starter Pack

20113839

Illumina Human Comprehensive Panel (24 samples)

20113836

Illumina Human Comprehensive Panel (96 samples)

20113837

Index Adapters

Illumina Unique Dual Indexes, LT (48 indexes, 48 samples)

20098166

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20091654

Price
 
 

Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20091656

Price
 
 

Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20091658

Price
 
 

Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20091660

Price
 
 

Data Analysis & Storage

Illumina Analytics - 1 iCredit

20042038

Price
 
 

Illumina Analytics  Starter Pack - 1,000 iCredits

20042039

Price
 
 

Illumina Analytics  - 5,000 iCredits

20042040

Price
 
 
What accessories do I need?
What accessories do I need?

Illumina DNA PCR-Free Prep, Tagmentation (24 Samples)

20041794

Price
 
 

Illumina DNA PCR-Free Prep, Tagmentation (96 Samples)

20041795

Price
 
 

Product Highlights

Illumina Complete Long Reads with Enrichment, Human enables highly accurate, cost-effective, targeted long reads from low input DNA, using your existing Illumina sequencing systems. This library prep kit adds cost-effective insights by utilizing long reads where they provide the most value by complementing a standard, short-read whole genome with the targeted long-read panel of choice. Note: Analysis requires standard, ≥ 30× short-read WGS data from the same sample to complement the targeted long reads.

Long reads where you need them
  • Highly flexible, targeted long reads compatible with premanufactured, predesigned, or custom panels
  • Targeted long reads with N50 5-7 kb to provide enhanced coverage of challenging-to-map regions of the human genome
  • Cost-effective insights by providing targeted long reads where they provide the most value
  • Accessible with standard NGS library prep workflow and your Illumina NovaSeq X, NovaSeq 6000, NovaSeq 6000Dx (RUO mode) systems
  • Analyzed in combination with ≥ 30× standard short read human whole genome using a streamlined and comprehensive DRAGEN app

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NovaSeq 6000 System Human Comprehensive Panel: 24 samples per S4 flow cell Large panels (15–25 Mb): ~75 samples per S4 flow cell Medium panels (5–15 Mb): ~187 samples per S4 flow cell Small panels (> 5 Mb): ~375 samples per S4 flow cell 2 × 150
NovaSeq X System Human Comprehensive Panel: 24 samples per 10B flow cell, 64 samples per 25B flow cell Large panels (15–25 Mb): ~75 samples per 10B flow cell, ~200 samples per 25B flow cell Medium panels (5–15 Mb): ~187 samples per 10B flow cell, ~500 samples per 25B flow cell Small panels (> 5 Mb): ~375 samples per 10B flow cell, ~1000 samples per 25B flow cell 2 × 150
NextSeq 2000 System Human Comprehensive Panel: 3 samples per P3 flow cell Large panels (15–25 Mb): ~9 samples per P3 flow cell Medium panels (5–15 Mb): ~22 samples per P3 flow cell Small panels (> 5 Mb): ~45 samples per P3 flow cell 2 × 150

Product Comparison

Illumina Complete Long Reads with Enrichment, Human Illumina Complete Long Read Prep, Human Illumina DNA Prep with Exome 2.5 Enrichment Illumina DNA PCR-Free Prep
Assay Time ~2 Days Approximately 7.5–8.5 hours 6.5 hours ~1.5 hours
Description Flexible, cost-effective targeted long reads coupled with short-read data for highly accurate WGS. Pair with a pre-designed or custom panel to resolve challenging-to-map regions of the genome or add phased sequencing to regions of interest. Illumina Complete Long Read Prep, Human performs long-read human germline whole-genome sequencing using existing Illumina NovaSeq platforms. A fast and easy-to-use library prep with enrichment workflow with a focused enrichment probe panel of up-to-date exome content for cost-effective and reliable human whole-exome sequencing.
Hands-On Time ~6 hours 5–6 hours including (PCR and QC) ~2 hours ~45 minutes
Input Quantity 50 ng, recommended, as low as 10 ng Recommended 50 ng DNA 50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide) 25 ng to 300 ng
Method Custom Sequencing, Long-Read Sequencing, Phased Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Whole-Genome Sequencing Long-Read Sequencing, Whole-Genome Sequencing Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing De Novo Sequencing, Whole-Genome Sequencing
Multiplexing Up to 384 samples per lane Up to 48 Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible. Up to 384-plex
Nucleic Acid Type DNA DNA DNA DNA
Number of Reactions 24 or 96 8 or 24
Specialized Sample Types Blood, Not FFPE-Compatible, Saliva Blood, Low-Input Samples, Not FFPE-Compatible, Saliva Blood, Saliva Blood, Low-Input Samples, Not FFPE-Compatible, Saliva
System Compatibility NovaSeq 6000, NovaSeq 6000Dx in Research Mode, NovaSeq X, NovaSeq X Plus NovaSeq 6000, NovaSeq 6000Dx, NovaSeq X, NovaSeq X Plus HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000, NovaSeq X, NovaSeq X Plus MiSeq, MiSeqDx in Research Mode, NextSeq 500, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000, NovaSeq X, NovaSeq X Plus
Technology Sequencing Sequencing Sequencing Sequencing

Supporting Data and Figures

 

 

Related Products