TruSeq DNA Methylation Kit

Converts bisulfite-treated, single-stranded DNA into an Illumina sequencing library. Enables comprehensive whole-genome DNA methylation analysis. Read More...
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TruSeq DNA Methylation Kit (12 reactions)



TruSeq DNA Methylation Kit (24 reactions)



TruSeq DNA Methylation Kit (96 reactions)


Accessory Products
What accessories do I need?

TruSeq DNA Methylation Index PCR Primers (10 reactions, 12 indexes)



Product Highlights

  • Unlock small samples (50–100 ng DNA input)
  • CpG, CHH, & CHG regions included for comprehensive, whole-genome results
  • Fast protocol – five-hour method
  • Capture full sample diversity
Sequence the entire sample–no loss of information

The process of bisulfite treatment denatures genomic DNA into single stranded DNA. TruSeq DNA Methylation converts single stranded DNA into an Illumina sequencing library. All ssDNA fragments are captured into an Illumina sequencing library, therefore eliminating the loss of diversity associated with other methods.

Supported analysis in the cloud

TruSeq DNA Methylation libraries can be aligned to the human genome and compared for differential methylation with Illumina BaseSpace Apps MethylSeq and MethylKit. These applications are fully supported and were developed specifically for TruSeq DNA Methylation library preparation.

Example data sets for TruSeq DNA Methylation libraries, also available in BaseSpace Sequence Hub Data Central (use the “Methyl Seq” category filter), demonstrate unparalleled quality and seamless analysis.

Deep coverage of critical genomic regions

Depth of coverage is enhanced in genomic areas with biological utility. TruSeq DNA Methylation captures full sample diversity of critical areas, including:

  • Coding region start and end for exons from the canonical transcript of protein coding genes for genes known to be involved in cancer, taken from SOMA and CRUK panels (as well as literature-derived cancer genes)
  • Genes defined by the American College of Medical Genetics as being medically relevant (ACMG genes)
  • Exonic coding regions from Ensemble 70
  • List of 100 promoters defined by the Broad Institute as being of high interest and difficult to sequence


Method-Specific Workflow Example


Customer Stories

The Mysteries of Metastasis

Researchers investigate the epigenetics behind cancer metastasis using the HumanMethylation450 BeadChip and the HiSeq System.

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Searching for the Epigenetic Contributions of Addiction

Targeted methylation sequencing using the MiSeq System and Nextera XT Library Prep Kit is identifying epigenetic signatures that correlate with brain image scans of substance abusers.

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Supporting Data and Figures


Product Literature

TruSeq DNA Methylation Kit

Data Sheet | PDF < 1 MB

Manuals and Support Information

TruSeq DNA Methylation Kit Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq DNA Methylation Kit Support 

Related Products

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TruSeq ChIP Library Preparation Kit

Simple, cost-effective chromatin immunoprecipitation sequencing (ChIP-Seq) DNA library preparation, with master mixes and robust multiplex capabilities.

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TruSeq Methyl Capture EPIC Library Prep Kit

This kit combines next-generation sequencing with epigenetic insights to accelerate biomarker discovery and understand methylation’s role in gene regulation.

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Infinium MethylationEPIC Kit

Robust methylation profiling microarray with extensive coverage of CpG islands, genes, and enhancers. Use for epigenome-wide association studies.

TruSeq DNA Methylation was previously known as the EpiGnome Library Preparation Kit. Kits with either name on the label contain the same quality reagents and follow the same workflow.