High Output Run Mode*
| HiSeq SBS V4 | ||||
|---|---|---|---|---|
| Read length | 1 × 36 bp | 2 × 50 bp | 2 × 100 bp | 2 × 125 bp |
| Dual Flow Cell | 128–144 Gb | 360–400 Gb | 720–800 Gb | 900 Gb–1 Tb |
| Single Flow Cell | 64–72 Gb | 180–200 Gb | 360–400 Gb | 450–500 Gb |
| Dual Flow Cell Run Time | 29 hrs | 2.5 days | 5 days | 6 days |
| TruSeq SBS V3 | ||||
| Read length | 1 × 36 bp | 2 × 50 bp | 2 × 100 bp | 2 × 125 bp |
| Dual Flow Cell | 95–105 Gb | 270–300 Gb | 540–600 Gb | N/A |
| Single Flow Cell | 47–52 Gb | 135–150 Gb | 270–300 Gb | N/A |
| Dual Flow Cell Run Time | 2 days | 5.5 days | 11 days | N/A |
| HiSeq SBS V4 | ||
|---|---|---|
| Flow Cell Type | Dual Flow Cell | Single Flow Cell |
| Reads Passing Filter (8 lanes per flow cell) | Up to 4 billion single read or 8 billion paired-end reads | Up to 2 billion single read or 4 billion paired-end reads |
| Quality |
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| TruSeq SBS V3 | ||
| Flow Cell Type | Dual Flow Cell | Single Flow Cell |
| Reads Passing Filter (8 lanes per flow cell) | Up to 3 billion single read or 6 billion paired-end reads | Up to 1.5 billion single read or 3 billion paired-end reads |
| Quality |
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*Install specifications based on Illumina PhiX control library at supported cluster densities (between 610–678 K clusters/mm2 passing filter using TruSeq v3 Kits or 870–930 K clusters/mm2 passing filter using HiSeq v4). Run times for high output mode correspond to sequencing only. Performance may vary based on sample quality, cluster density, and other experimental factors.
The HiSeq 2500 System has been obsolesced. We intend to continue to provide full support of the instrument and supply the reagents through February 28th, 2023. We also offer several instruments that support the same applications as the HiSeq 2500 System; we recommend using the Sequencing Platform Comparison Tool to find the best instrument for your needs.
Rapid Run Mode†
| HiSeq Rapid SBS Kit V2 | |||||
|---|---|---|---|---|---|
| Read length | 1 × 36 bp | 2 × 50 bp | 2 × 100 bp | 2 × 150 bp | 2 × 250 bp |
| Dual Flow Cell | 18–22 Gb | 50–60 Gb | 100–120 Gb | 150–180 Gb | 250–300 Gb |
| Single Flow Cell | 9–11 Gb | 25–30 Gb | 50–60 Gb | 75–90 Gb | 125–150 Gb |
| Dual Flow Cell Run Time | 7 hr | 16 hr | 27 hr | 40 hr | 60 hr |
| HiSeq Rapid SBS Kit V2 | |||||
|---|---|---|---|---|---|
| Flow Cell Type | Dual Flow Cell | Single Flow Cell | |||
| Reads Passing Filter (2 lanes per flow cell) | Up to 600 million single read or 1.2 billion paired-end reads | Up to 300 million single read or 600 million paired-end reads | |||
| Quality |
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† Install specifications based on Illumina PhiX control library at supported cluster densities (between 700–820 K clusters/mm2 passing filter using HiSeq Rapid v2 Kits). Run times for rapid run mode correspond to on-board cluster generation (1.5 hr) and sequencing. Performance may vary based on sample quality, cluster density, and other experimental factors. Early HiSeq 2000 instruments will run slightly slower when upgraded to a HiSeq 2500.
HiSeq 2500 System Specification Sheet
Power and efficiency for production-scale sequencing.
View Specification SheetKey Technology
Sequencing by Synthesis (SBS) Technology
The HiSeq 2500 System harnesses proven Illumina SBStechnology to deliver highly accurate data and robust performance for a broad range of applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
Learn morePaired-End Sequencing Technology
Paired-end sequencing enables both ends of the DNA fragment to be sequenced. Because the distance between each paired read is known, alignment algorithms can use this information to map the reads over repetitive regions more precisely. This results in much better alignment of the reads, especially across difficult-to-sequence, repetitive regions of the genome.
Learn more