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TruSight Oncology 500 High-Throughput

Enabling comprehensive genomic profiling from FFPE samples, with added flexibility to batch up to 192 samples per flow cell. Read More...

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Manual Prep

TruSight Oncology 500 DNA High-Throughput (48 Samples)

20040765

Price
 
 

TruSight Oncology 500 DNA High-Throughput Kit (144 Samples)

20040767

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput (24 Samples)

20040764

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput Kit (72 Samples)

20040766

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput Kit (24 Samples), plus Pierian

20040768

Price
 
 

TruSight Oncology 500 DNA High-Throughput Kit (48 Samples), plus Pierian

20040769

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput Kit (72 Samples), plus Pierian

20040770

Price
 
 

TruSight Oncology 500 DNA High-Throughput Kit (144 Samples), plus Pierian

20040771

Price
 
 
Automation

TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples)

20049283

Price
 
 

TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples)

20049282

Price
 
 

TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples), Plus Pierian

20049277

Price
 
 

TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples), Plus Pierian

20049276

Price
 
 

TruSight Oncology 500 High-Throughput DNA for Automation (144 Samples)

20049285

Price
 
 

TruSight Oncology 500 High-Throughput DNA for Automation (144 Samples), Plus Pierian

20049279

Price
 
 

TruSight Oncology 500 High-Throughput DNA/RNA for Automation (72 Samples)

20049284

Price
 
 

TSO500 DNA/RNA HT Auto (72 spls) + Pierian

20049278

Price
 
 
Index Adapters

IDT® for Illumina® UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)

20034701

Price
 
 

IDT® for Illumina® UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)

20034702

Price
 
 

IDT for Illumina UMI DNA/DNA Index Anchors Set A for Automation

20066404

Price
 
 

IDT for Illumina UMI DNA/DNA Index Anchors Set B for Automation

20063213

Price
 
 
Reagents

NovaSeq 6000 S4 Reagent Kit v1.5 (200 cycles)

20028313

Price
 
 

NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles)

20028315

Price
 
 

NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles)

20028318

Price
 
 

NovaSeq 6000 SP Reagent Kit v1.5 (200 cycles)

20040719

Price
 
 
Services

Trusight Oncology 500 HT Training (DNA) - Customer Site

20042220

Price
 
 

Trusight Oncology 500 HT Training (DNA and RNA) - Customer Site

20042219

Price
 
 
Software and Informatics Options

Illumina DRAGEN Server v3

20040619

Price
 
 

Illumina DRAGEN Server v4

20051343

Price
 
 

ICA Basic Annual Subscription

20044874

ICA Professional Annual Subscription

20044876

ICA Enterprise Annual Subscription

20038994

ICA Enterprise Service and Compliance Add-on (applies to Basic only)

20066830

Illumina Analytics - 1 iCredit

20042038

Price
 
 

Illumina Analytics  Starter Pack - 1,000 iCredits

20042039

Price
 
 

Illumina Analytics  - 5,000 iCredits

20042040

Price
 
 

Illumina Analytics  - 50,000 iCredits

20042041

Price
 
 

Illumina Analytics - 100,000 iCredits

20042042

Price
 
 

Illumina Connected Insights-Annual Subscription

20090137

Price
 
 

Illumina Connected Insights‒Oncology Genome Equivalent Sample-VCF

20090138

Price
 
 

Illumina Connected Insights‒Training and Onboarding

20092376

Price
 
 

Informatics Professional Services

20071787

Price
 
 
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TruSight Oncology 500 High-Throughput

Product Highlights

The TruSight Oncology 500 High-Throughput assay is built on the foundation of the TruSight Oncology 500 assay, with flexibility for higher sample throughput and batching options.

Consolidate Multiple Biomarker Assays into One

  • Pan-cancer biomarker content aligned with key guidelines and clinical trials
  • 523 genes for assessment of all DNA and RNA variant types, plus microsatellite instability (MSI) and tumor mutational burden (TMB)
  • Increase chances of finding a positive biomarker by moving from individual biomarker assays to a single comprehensive NGS assay

Streamlined Sample-to-Results Workflow

  • Go from initial sample to results in 4-5 days
  • Rapid variant calling algorithm powered by DRAGEN pipelines, available locally on DRAGEN server
  • Secure, scalable, cloud-based variant calling is available with DRAGEN pipelines on Illumina Connected Analytics (ICA) featuring auto-launch capability.
  • Multiple verified tertiary analysis options available, including Illumina Connected Insights‡ and Pierian Clinical Genomics Workspace

High Sample Throughput

  • Flexible sample batching allows you to run anywhere from 16 to 192 samples per sequencing flow cell on the NovaSeq 6000 or NovaSeq 6000Dx System in RUO Mode†
  • Automation kits and methods available for reduced hands-on time and scalability*

Achieve Highly Confident Results

  • Hybrid-capture chemistry combined with sophisticated bioinformatics, leading to high analytical specificity and sensitivity
  • Inclusive of unique molecular indexes (UMIs) for high sensitivity in variant detection
  • Based on proven Illumina SBS sequencing technology

Enable In-House Comprehensive Genomic Profiling

  • Keep samples and data in house, for more control and ability to build a database
  • A future-proof assay with emerging biomarkers already included
Learn more about the TruSight Oncology Product Family

*Automation available on the Beckman Coulter i-Series or the Hamilton Microlab STAR. Contact Illumina Sales for more information.

†NovaSeq 6000Dx System in RUO Mode requires a separate, stand-alone DRAGEN server for secondary analysis.

‡Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.

TruSight Oncology 500 Portfolio

All three TruSight Oncology 500 assays are designed to identify key biomarkers in guidelines and clinical trials.

TruSight Oncology 500 Assay

Assess key cancer biomarkers from FFPE tumor tissue with the TruSight Oncology 500 Assay.

TruSight Oncology 500 High-Throughput Assay

Batch up to 192 samples at a time while using the same biomarkers and tissue type as the TruSight Oncology 500 assay.

TruSight Oncology 500 ctDNA Assay

Analyze circulating tumor DNA from plasma with the TruSight Oncology 500 ctDNA assay.

Frequently Purchased Together

Specifications

Product Comparison

TruSight Oncology 500 High-Throughput TruSight Oncology 500 TruSight Oncology 500 ctDNA
Cancer Type Pan-Cancer Pan-Cancer Pan-Cancer
Content Specifications Targeted selection of DNA from 523 genes of interest, and RNA from 55 genes, for a total of 1.94Mb panel size.
• Immuno-oncology Biomarker Coverage: TMB and MSI
• Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types
• Clinical Trials Coverage: Over 600 clinical trials*		 Targeted selection of DNA from 523 genes of interest, and RNA from 55 genes, for a total of 1.94Mb panel size.
• Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types
• Clinical Trials Coverage: Over 1,000 clinical trials
• Immuno-oncology Biomarker Coverage: Biomarkers TMB and MSI included; also inclusive of HLA regions, POLE1 and POLD1*
• TruSight Oncology 500 HRD** kit content includes coverage of ~25,000 SNP's to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST), powered by Myriad Genetics
• TruSight Oncology 500 HRD** is an optional add-on kit to TruSight Oncology 500

**Not available in Japan		 Targeted selection of 523 genes (full coding sequence) for a total of 1.94Mb panel size.
• Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types
• Immuno-oncology Biomarker Coverage: TMB and MSI*
Hands-On Time Manual: ~10.5 hrs
Automated: ~2.5 hrs		 Manual: ~10.5 hrs
Automated: ~2.5 hrs (TruSight Oncology 500 only)		 Manual: ~10.5 hrs
Automated: N/A
Input Quantity 40 ng DNA, 40-80 ng RNA (need at least 2 mm3 FFPE tissue) 40 ng DNA, 40 ng RNA 30 ng cfDNA (8-10 ml of plasma)
Method Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing Target Enrichment, Target Enrichment, Targeted DNA Sequencing
Nucleic Acid Type RNA, DNA RNA, DNA DNA
Sample Throughput 16–192 samples per flow cell
Specialized Sample Types FFPE Tissue FFPE Tissue Blood, Circulating Tumor DNA
Species Category Human Human Human
System Compatibility NovaSeq 6000, NovaSeq 6000Dx in Research Mode NextSeq 500, NextSeq 550, NextSeq 550Dx in Research Mode NovaSeq 6000
Technology Sequencing Sequencing Sequencing
Variant Class Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Somatic Variants

* Based on Pierian clinical knowledgebase, as of February 2023.

† NovaSeq 6000Dx System in RUO Mode requires a separate, stand-alone DRAGEN server for secondary analysis.

Method-Specific Workflow Example

 
Prepare Library for:
TruSight Oncology 500 DNA High-Throughput Assay
 
Sequence Efficiently with:
NovaSeq 6000 System
NovaSeq 6000Dx in RUO Mode
 
Analyze Your Data with:

Variant Calling:

DRAGEN Bio-IT Platform: on-premise via DRAGEN server
DRAGEN Bio-IT Platform: cloud-based via Illumina Connected Analytics, ICA
TruSight Oncology 500 Local App: Docker-based Software

Insights and report generation:

Illumina Connected Insights*
Compatible with Pierian Clinical Genomics Workspace Software

* Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.

Supporting Data and Figures

 

Product Literature

Simplifying the process to identify applicable variants in oncology samples

Brochure | PDF 2 MB

Optimizing RNA input to detect gene fusions with TruSight Oncology 500 High-Throughput

Technical Note | PDF < 1 MB

Data analysis and reporting for the TruSight Oncology 500 portfolio

Product Information Sheet | PDF 1 MB

Data Sheet | PDF | 7 versions

Manuals and Support Information

DRAGEN TruSight Oncology 500 Analysis Software v1.1 Documentation

TruSight Oncology 500 v1.0 Local App Documentation

TruSight Oncology 500 v2.0 Local App Documentation

TruSight Oncology 500 v2.1 Local App Documentation

DRAGEN TruSight Oncology 500 Analysis Software v2.1 Documentation

TruSight Oncology 500 High Throughput Reference Guide Documentation

DRAGEN TruSight Oncology 500 Analysis Software v1.1 on ICA Documentation

DRAGEN TruSight Oncology 500 Analysis Software v2.1.1 on ICA Documentation

TruSight Oncology 500 High Throughput Consumables & Equipment Documentation

TruSight Oncology 500 High Throughput Checklist Documentation

TruSight Oncology 500 v2.2 Local App Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

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Not for use in diagnostic procedures (except as specifically noted).

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