Key Applications and Methods
With incredible speed and throughput, the HiSeq X Ten System was purpose-built for population-scale whole-genome sequencing (WGS). The HiSeq X Ten empowers scientists, institutions, and nations with the ability to create a comprehensive catalog of human and species variation, forge population-based references, drive far-reaching discoveries, and advance our understanding of biology and human genetic disease.
See how human WGS on the HiSeq X Ten is impacting the future of medicine.
Prep
5–6 hours/4–5 hours hands-on
TruSeq DNA PCR-Free High Throughput Library Preparation Kit
Simple, all-inclusive WGS library preparation that provides accurate and comprehensive coverage of complex genomes.
TruSeq Nano DNA High Throughput Library Prep Kit
Efficient sequencing of samples with limited available DNA.
Illumina DNA Prep
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Sequence
< 3 days
cBot 2 System
Automates the cluster generation process.
HiSeq X Reagent Kits
SBS reagents, clustering reagents, and patterned flow cells for increased cluster density and simplified image analysis, compared to non-patterned flow cells.
Analyze
5–7 hours
HiSeq Analysis Software
Reduces data analysis bottlenecks and challenges for high-throughput whole-genome sequencing.
BaseSpace Whole-Genome Sequencing App
Enables rapid alignment, variant calling, and annotation for whole genome sequencing data.
Browse sample data on BaseSpace Sequence Hub:
HiSeq X: PCR-Free Example Data
HiSeq X: TruSeq Nano Example Data
Estimated Cost Per Human Genome: $1000* at 18,000 genomes per year
*When used at scale, the HiSeq X Ten delivers a $1000 human 30x coverage genome, inclusive of instrument depreciation, DNA extraction, library preparation, and estimated labor for a typical high-throughput genomics laboratory. Calculated in 2014.
Apply population-scale whole-genome sequencing (WGS) to non-human species. The HiSeq X Series delivers high coverage in a variety of areas, including agriculture and model organism research in the pharmaceutical industry.
Sequencing large complex genomes provides a base-by-base view of genomic alterations to inform studies of disease or population genomics.
Prep
5–6 hours/4–5 hours hands-on
TruSeq DNA PCR-Free Library Preparation Kit
Simple, all-inclusive WGS library preparation that provides accurate and comprehensive coverage of complex genomes.
TruSeq Nano DNA Library Prep Kit
Efficient sequencing of samples with limited available DNA.
Illumina DNA Prep
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Sequence
< 3 days
cBot 2 System
Automates the cluster generation process.
HiSeq X Reagent Kits
SBS reagents, clustering reagents, and patterned flow cells for increased cluster density and simplified image analysis, compared to non-patterned flow cells.
Analyze
Varies by species, coverage
Sequencing Analysis Viewer
Monitor quality metrics from sequencing runs in real-time from almost any location.
BaseSpace BWA Aligner App
Align samples to a reference genome, including a custom reference genome created from imported FASTA files.
Make Operational Excellence a Reality with Your HiSeq X Series
Illumina SeqLab for large-scale WGS on the HiSeq X Series helps accelerate your NGS production and expertise.
Learn More