Key Applications and Methods
With incredible speed and throughput, the HiSeq X Ten System was purpose-built for population-scale whole-genome sequencing (WGS). The HiSeq X Ten empowers scientists, institutions, and nations with the ability to create a comprehensive catalog of human and species variation, forge population-based references, drive far-reaching discoveries, and advance our understanding of biology and human genetic disease.
See how human WGS on the HiSeq X Ten is impacting the future of medicine.
Prep
5–6 hours/4–5 hours hands-on
TruSeq DNA PCR-Free Library Preparation Kit
Simple, all-inclusive WGS library preparation that provides accurate and comprehensive coverage of complex genomes.
TruSeq Nano DNA Library Prep Kit
Efficient sequencing of samples with limited available DNA.
Illumina DNA Prep
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Sequence
< 3 days
cBot 2 System
Automates the cluster generation process.
HiSeq X Reagent Kits
SBS reagents, clustering reagents, and patterned flow cells for increased cluster density and simplified image analysis, compared to non-patterned flow cells.
Analyze
5–7 hours
HiSeq Analysis Software
Reduces data analysis bottlenecks and challenges for high-throughput whole-genome sequencing.
BaseSpace Whole-Genome Sequencing App
Enables rapid alignment, variant calling, and annotation for whole genome sequencing data.
Browse sample data on BaseSpace Sequence Hub:
HiSeq X: PCR-Free Example Data
HiSeq X: TruSeq Nano Example Data
Estimated Cost Per Human Genome: $1000* at 18,000 genomes per year
*When used at scale, the HiSeq X Ten delivers a $1000 human 30x coverage genome, inclusive of instrument depreciation, DNA extraction, library preparation, and estimated labor for a typical high-throughput genomics laboratory. Calculated in 2014.
Apply population-scale whole-genome sequencing (WGS) to non-human species. The HiSeq X Series delivers high coverage in a variety of areas, including agriculture and model organism research in the pharmaceutical industry.
Sequencing large complex genomes provides a base-by-base view of genomic alterations to inform studies of disease or population genomics.
Prep
5–6 hours/4–5 hours hands-on
TruSeq DNA PCR-Free Library Preparation Kit
Simple, all-inclusive WGS library preparation that provides accurate and comprehensive coverage of complex genomes.
TruSeq Nano DNA Library Prep Kit
Efficient sequencing of samples with limited available DNA.
Illumina DNA Prep
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Sequence
< 3 days
cBot 2 System
Automates the cluster generation process.
HiSeq X Reagent Kits
SBS reagents, clustering reagents, and patterned flow cells for increased cluster density and simplified image analysis, compared to non-patterned flow cells.
Analyze
Varies by species, coverage
Sequencing Analysis Viewer
Monitor quality metrics from sequencing runs in real-time from almost any location.
BaseSpace BWA Aligner App
Align samples to a reference genome, including a custom reference genome created from imported FASTA files.
Make Operational Excellence a Reality with Your HiSeq X Series
Illumina SeqLab for large-scale WGS on the HiSeq X Series helps accelerate your NGS production and expertise.
Learn More