Incredible speed and scale for whole-genome sequencing

The HiSeq X Ten and HiSeq X Five Systems generate a staggering level of throughput, sequencing thousands of genomes per year

HiSeq X Series Specifications

  Dual Flow Cell Single Flow Cell
Output per Run 1.6-1.8 Tb 800-900 Gb
Reads Passing Filter 5.3-6 billion 2.6-3 billion
Supported Read Length 2 × 150 bp
Run Time < 3 days
Quality Scores ≥ 75% of bases above Q30 at 2 × 150 bp
Supported Library Preparation TruSeq DNA PCR-Free Library Prep Kit, TruSeq Nano DNA Library Prep Kit

* Specifications based on Illumina PhiX control library at supported cluster densities (1255–1412 K clusters/mm2) on one HiSeq X System instrument. Supported library preparation kits include TruSeq Nano DNA and TruSeq DNA PCR-Free, with a 350 bp target insert size and HiSeq X Reagent Kit v2. The HiSeq X System was designed, optimized, and licensed for whole-genome sequencing. Other applications are not compatible.

  HiSeq X Ten System HiSeq X Five System
Minimum Number of Instruments 10 5
Annual Genome Capacity > 18,000 > 9000
Price per 30x Genome < $1000 < $1500
HiSeq X Series Specification Sheet

HiSeq X Series Specification Sheet

Learn how the HiSeq X Series delivers exceptional throughput and low cost for population-scale whole-genome sequencing.

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Sequencing by Synthesis Technology

The HiSeq X Ten and HiSeq X Five Systems leverage proven Illumina sequencing by synthesis (SBS) technology, the most widely adopted next-generation sequencing (NGS) technology in the industry. This chemistry ensures high data quality. SBS technology supports massively parallel sequencing using a proprietary fluorescently labeled reversible-terminator method.

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Patterned Flow Cell Technology

The HiSeq X Series incorporates patterned flow cell technology to generate an exceptional level of throughput for whole-genome sequencing. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output of the system.

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See patterned flow cells in action