The OvineSNP50 BeadChip array features over 54,241 evenly spaced probes that target SNPs, offering more than sufficient SNP density for genome-wide association studies and other applications such as genome-wide selection, determination of genetic merit, identification of quantitative trait loci, and comparative genetic studies.
The BeadChip was developed in collaboration with leading ovine researchers from AgResearch, Baylor UCSC, CSIRO, and the USDA as part of the International Sheep Genomics Consortium. It features over 54,241 evenly spaced probes that target single nucleotide polymorphisms (SNPs).
More than 18,000 of these markers were discovered through sequencing reduced representation libraries with the Illumina Genome Analyzer IIx. A set of 600 SNPs were identified by BAC end sequencing and validated with Illumina GoldenGate Genotyping Assays, for over 403 animals from 23 breeds. The remaining SNPs were derived from the draft ovine genome.
The OvineSNP50 BeadChip provides uniform genome-wide coverage with an estimated one marker per 46kb on average. The BeadChip provides high call rates, allows for flexible content deployment, and enables the detection and measurement of copy number variation.
- The BeadChip further reduces experimental variability by allowing researchers to interrogate up to 12 samples in parallel
- The assay's single-tube sample preparation without PCR or ligation steps significantly reduces labor and potential sample handling errors
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