MiSeq Reagent Kit v3

Optimized chemistry to increase cluster density and read length, and improve sequencing quality scores, compared to earlier MiSeq reagent kit versions.Read More...
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MiSeq Reagent Kit v3 (150-cycle)



MiSeq Reagent Kit v3 (600-cycle)


Illumina Advantage Products

TG MiSeq Reagent Kit v3 (600 cycle)



TG MiSeq® Reagent Kit v3 (150 cycle)


How to Achieve More Consistent Cluster Density on Illumina Sequencing Platforms

Achieving optimal cluster density is critical to high-quality sequencing on MiniSeq, MiSeq, NextSeq, and HiSeq 2500 Systems. Read more about how to achieve consistent cluster density in order to streamline data outputs.

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Product Highlights

MiSeq Reagent Kits v3 enable the highest output of all MiSeq kits. The v3 kits retain the same pre-filled, ready-to-use reagent cartridges as the v1 and v2 kits, but offer improved chemistry to increase cluster density and read length as well as improve quality (Q) scores.

MiSeq Reagent Kit v3 allows researchers to:

  • Double the output of a single run
  • Extend read lengths up to 2 x 300 bp with the 600-cycle kit
  • Increase the number of reads to 25 million to unlock new applications
Updated system software unlocks the full benefits of the v3 kits. Access the latest software.


The MiSeq v3 kits can achieve double the amount of output per flow cell. Kits are available in a 600-cycle format to allow the longest read lengths on any Illumina sequencing system. There is also a 150-cycle format that enables counting applications. In addition, all MiSeq Reagent Kit v2 configurations will continue to be available. MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

Reagent Kit No. of Reads Kit Size (cycles) Output (max.) 2 × 75 Output 2 × 300 Output
MiSeq Reagent Kit v3 25 M 150, 600 15 Gb 3.8 Gb 15 Gb

Gb = gigabases, M = millions

This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

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Best Practices for Storing Sequencing Reagents

Sequencing reagent performance depends on thawing technique, thawed storage time, and storage conditions. Learn how to thaw and store sequencing reagents for optimal performance.

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Best Practices for Storing Sequencing Reagents
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