The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing.
Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.
Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.
Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.
This kit has been validated for human and mouse samples. The BaseSpace Single-Cell RNA-Seq App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.
Single-Cell RNA Data Analysis Workflow
Technical Note | PDF < 1 MB
Illumina | Bio-Rad Single-Cell RNA Sequencing Solution
Application Note | PDF 1 MB
Illumina Bio-Rad Single-Cell RNA Sequencing Solution
Data Sheet | PDF < 1 MB
SureCell WTA 3' Library Prep Kit Documentation
Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.