Reagent and Library Prep Kit Bundles Available for the MiniSeq System

Order your MiniSeq reagents and library prep kits together for additional savings and convenience. Select any of item numbers 20005613 through 20005616, and add to your cart.

TruSeq Small RNA Library Preparation Kits

These kits provide a simple, cost-effective solution for generating miRNA and small RNA sequencing libraries directly from total RNA, for any species. Read More...
Select Product(s)

TruSeq Small RNA Library Prep Kit -Set A (24 rxns) (Set A: indexes 1-12)



TruSeq Small RNA Library Prep Kit -Set B (24 rxns) (Set B: indexes 13-24)



TruSeq Small RNA Library Prep Kit -Set C (24 rxns) (Set C: indexes 25-36)



TruSeq Small RNA Library Prep Kit -Set D (24 rxns) (Set D: indices 37-48)



TruSeq Small RNA Set A MiniSeq Kit



TruSeq Small RNA Set B MiniSeq Kit



TruSeq Small RNA Set C MiniSeq Kit



TruSeq Small RNA Set D MiniSeq Kit



Product Highlights

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. Compatible applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:


Method-Specific Workflow Example


Product Literature

Literature is not currently available for this product.

Manuals and Support Information

TruSeq Small RNA Library Prep Kit Documentation  

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq Small RNA Library Prep Kit Support 

Related Products

Image Source
MiniSeq Reagent Kit

MiniSeq Reagent Kits bring the power of Illumina NGS to labs of all sizes. Access cost-efficient sequencing, even for low numbers of samples.

Image Source
TruSeq DNA Methylation Kit

Converts bisulfite-treated, single-stranded DNA into an Illumina sequencing library. Enables comprehensive whole-genome DNA methylation analysis.

Image Source
TruSeq Targeted RNA Expression Library Prep Kits

Targeted RNA assays for gene expression studies: custom sequencing panels with a simple workflow and automated data analysis.