Reduced-representation bisulfite sequencing (RRBS-Seq ) or single-cell reduced-representation bisulfite sequencing (scRRBS ) is a protocol that uses one or multiple restriction enzymes on the genomic DNA to produce sequence-specific fragmentation. The fragmented genomic DNA is then treated with bisulfite and sequenced. This is the method of choice to study specific regions of interest. It is particularly effective where methylation is high, such as in promoters and repeat regions.
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