MiSeqDx

MiSeqDx

Next-generation sequencing (NGS) technology can provide a broad view of the human genome, helping us discover and apply relevant genomic information to human health. Ultimately, this may lead to more accurate diagnosis, prognosis, and therapeutic selection. Clinical laboratories can take advantage of the benefits of NGS with the FDA-cleared MiSeqDx system.

At the heart of the system is the FDA-cleared MiSeqDx instrument, stringently validated for design and performance. Two IVD assays and one IVD kit are currently available for use on the MiSeqDx instrument:

  • The MiSeqDx Universal Kit allows users to develop their own NGS laboratory tests, expanding their lab offerings to meet ever-evolving needs
  • The MiSeqDx Cystic Fibrosis 139-Variant Assay detects 139 clinically relevant and functionally verified CFTR variants
  • The MiSeqDx Cystic Fibrosis Clinical Sequencing Assay detects mutations within the protein coding regions and intron/exon boundaries of CFTR

MiSeq FGx

MiSeq FGx

The MiSeq FGx Forensic Genomics System is the first fully validated sequencing system designed for use in forensic genomics applications. The complete sample-to-answer system uses dedicated library preparation kits, customized instrument control software, and an analytical software package to support the reliable analysis of both routine and challenging forensic samples.

The MiSeq FGx System workflow is easy-to-use, automated, and fast:

  • Compact, all-in-one platform incorporates cluster generation, paired-end fluidics, and SBS chemistry
  • Intuitive touch screen interface makes for simple instrument operation
  • Plug-and-play reagents with RFID tracking make for added speed and convenience
  NovaSeq System HiSeq X Series*
  NovaSeq 6000 HiSeq X Five HiSeq X Ten
Output Range 167–6000 Gb 900–1800 Gb 900–1800 Gb
Run Time 19–40 hr <3 days <3 days
Reads per Run 1.4–20 billion§ 3–6 billion 3–6 billion
Maximum Read Length 2 × 150 bp 2 × 150 bp 2 × 150 bp
Samples per Run| 4–48 8–16 8–16
Relative Price per Sample| Higher Cost Higher Cost Lower Cost
Relative Instrument Price| Lower Cost Lower Cost Higher Cost
Downloads Spec Sheet Spec Sheet Spec Sheet

* Specifications shown for an individual HiSeq X Sequencing System. HiSeq X is only available as part of the HiSeq X Five or HiSeq X Ten.

† For Research Use Only. Not for use in diagnostic procedures.

‡ Output Range is based on a dual flow cell run of S4 Flow Cells which have not been released; therefore, performance metrics are subject to change.

§ Reads Per Run is based on a dual flow cell run of S4 Flow Cells which have not been released; therefore, performance metrics are subject to change.

| Based on 30X coverage of a human genome, assumes minimum of 100Gb per genome. Sample number will vary for other species based on relative genome size.

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  MiniSeq System MiSeq Series NextSeq System HiSeq System NovaSeq Series††
  MiniSeq MiSeq* MiSeqDx MiSeq FGx NextSeq 500* HiSeq 4000* NovaSeq 5000* NovaSeq 6000*
Output Range 1.8–7.5 Gb 0.3–15 Gb 0.3–15 Gb 0.3–15 Gb 20–120 Gb 125–1500 Gb 167—2000 Gb 167—6000 Gb
Run Time 4–24 hr 5–55 hr 4–55 hr 4–55 hr 11–29 hr <1–3.5 days TBA 19—40 hr
Reads per Run 8–25 million 1–25 million 1–25 million 1–25 million (for research) 130–400 million 2.5–5 billion 1.4–6.6 billion 1.4–20 billion
Max Read Length 2 × 150 bp 2 × 300 bp 2 × 300 bp 2 × 300 bp 2 × 150 bp 2 × 150 bp 2 × 150 bp 2 × 150 bp
Samples per Run§ N/A N/A N/A N/A 1 6–12 4—16 4—48
Samples per Run§ 1–384 1–384 N/A 1–96 96 768–1536 N/A N/A
Samples per Run§ N/A N/A N/A N/A 8 50—100 32—132 32—192
Samples per Run§ 1–96 1–96 N/A N/A 12–36 N/A N/A N/A
Samples per Run§ 1–384 1–384 N/A N/A 1–384 1–6144 N/A N/A
Samples per Run§ N/A N/A N/A N/A 2–8 50–100 32—132 32—192
Samples per Run§ 1–5 1–5 N/A N/A 25–80 60–792 N/A N/A
Samples per Run§ 1–6 1–6 N/A N/A 8–24 160–320 96—192 96—192
Samples per Run§ N/A N/A N/A N/A 1 3–6 2—8 2–24
Samples per Run§ 1–384 1–384 N/A N/A 1–384 N/A N/A N/A
Relative Price per Sample§ Higher Cost Higher Cost Higher Cost Higher Cost Mid Cost Lower Cost Lower Cost Lower Cost
Relative Instrument Price§ Lower Cost Lower Cost Lower Cost Lower Cost Mid Cost Higher Cost Higher Cost Higher Cost
Downloads Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet

†† All output and read number specifications based on dual flow cell runs. Run time includes cluster generation, sequencing, and base calling for a Dual S2 Flow Cell Run on the NovaSeq 6000 System. Run times on the NovaSeq 5000 System may be slightly longer.

* For Research Use Only. Not for use in diagnostic procedures.

† For In Vitro Diagnostic applications.

‡ For Research, Forensic, and Paternity Use Only.

§ Based on 30X coverage of a human genome, assumes minimum of 100Gb per genome. Sample number will vary for other species based on relative genome size.

§ Based on 30X or greater coverage of a 5Mb microbial genome. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ Based on 8 Gb per sample at 2x75 bp. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 50M reads at 2x75bp or greater.

§ Based on TruSight One Sequencing Panel. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 10 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 50 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 50M reads at 2x50bp or greater.

§ 5 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 15 million reads per sample for identification of transcription factor binding. Additional reads required for histone marks. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 15M reads at 1x50bp or greater.

§ 30X coverage. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 2x75 reads or greater.

§ Based on Nextera XT indexing. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

Sequencing Platform Comparison Tool

This tool will help you compare next-generation sequencing systems and find the one that's right for your lab or application

Let's begin with some information about the nature of your work.

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MiSeqDx System

Next-generation sequencing (NGS) technology can provide a broad view of the human genome, helping us discover and apply relevant genomic information to human health. Ultimately, this may lead to more accurate diagnosis, prognosis, and therapeutic selection. Clinical laboratories can take advantage of the benefits of NGS with the FDA-cleared MiSeqDx system.

At the heart of the system is the FDA-cleared MiSeqDx instrument, stringently validated for design and performance. Two IVD assays and one IVD kit are currently available for use on the MiSeqDx instrument:

  • The MiSeqDx Universal Kit allows users to develop their own NGS laboratory tests, expanding their lab offerings to meet ever-evolving needs
  • The MiSeqDx Cystic Fibrosis 139-Variant Assay detects 139 clinically relevant and functionally verified CFTR variants
  • The MiSeqDx Cystic Fibrosis Clinical Sequencing Assay detects mutations within the protein coding regions and intron/exon boundaries of CFTR

MiSeq FGx System

The MiSeq FGx Forensic Genomics System is the first fully validated sequencing system designed for use in forensic genomics applications. The complete sample-to-answer system uses dedicated library preparation kits, customized instrument control software, and an analytical software package to support the reliable analysis of both routine and challenging forensic samples.

The MiSeq FGx System workflow is easy-to-use, automated, and fast:

  • Compact, all-in-one platform incorporates cluster generation, paired-end fluidics, and SBS chemistry
  • Intuitive touch screen interface makes for simple instrument operation
  • Plug-and-play reagents with RFID tracking make for added speed and convenience

 
HiSeq X Five
Output Range 900-1800 Gb
Run Time < 3 days
Reads per Run 3-6 billion
Maximum Read Length 2 x 150 bp
Samples per Run§ 8-16
Relative Price per Sample§ Higher Cost
Relative Instrument Price§ Lower Cost
Downloads Spec Sheet
System Overview HiSeq Series Overview
 
HiSeq X Ten
Output Range 900-1800 Gb
Run Time < 3 days
Reads per Run 3-6 billion
Maximum Read Length 2 x 150 bp
Samples per Run§ 8-16
Relative Price per Sample§ Lower Cost
Relative Instrument Price§ Higher Cost
Downloads Spec Sheet
System Overview HiSeq Series Overview
 
NovaSeq 6000
Output Range 134-6000 Gb
Run Time 24-44 hr
Reads per Run Up to 20 billion
Maximum Read Length 2 x 150 bp
Samples per Run§ 4-48
Relative Price per Sample§ Higher Cost
Relative Instrument Price§ Lower Cost
Downloads Spec Sheet
System Overview NovaSeq Overview


  • Specifications shown for an individual HiSeq X Sequencing System. HiSeq X is only available as part of the HiSeq X Five or HiSeq X Ten.
  • For Research Use Only. Not for use in diagnostic procedures.
  • Lower limits of output and reads per run ranges based on a single S1 flow cell run; upper limits based on dual S4 flow cell run. Run times include cluster generation, sequencing, and base calling. Run times are based on running two flow cells of the same type. Starting two different flow cells will impact run time. NovaSeq S1 Reagent Kits are not currently available.
  • Based on 30X coverage of a human genome, assumes minimum of 100Gb per genome. Sample number will vary for other species based on relative genome size.
 
MiniSeq
Output Range 1.8-7.5 Gb
Run Time 4-24 hr
Reads per Run 8-25 million
Maximum Read Length 2 x 150 bp
Samples per Run N/A
Samples per Run 1-384
Samples per Run N/A
Samples per Run 1-96
Samples per Run 1-384
Samples per Run N/A
Samples per Run 1-5
Samples per Run 1-6
Samples per Run N/A
Samples per Run 1-384
Relative Price per Sample Higher Cost
Relative Instrument Price Lower Cost
Downloads Spec Sheet
System Overview MiniSeq Overview
 
MiSeq*
Output Range 0.3-15 Gb
Run Time 5-55 hr
Reads per Run 1-25 million
Maximum Read Length 2 x 300 bp
Samples per Run N/A
Samples per Run 1-384
Samples per Run N/A
Samples per Run 1-96
Samples per Run 1-384
Samples per Run N/A
Samples per Run 1-5
Samples per Run 1-6
Samples per Run N/A
Samples per Run 1-384
Relative Price per Sample Mid Cost
Relative Instrument Price Mid Cost
Downloads Spec Sheet
System Overview MiSeq Overview
 
MiSeq Dx
Output Range 0.3-15 Gb
Run Time 4-55 hr
Reads per Run 1-25 million
Maximum Read Length 2 x 300 bp
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Relative Price per Sample Mid Cost
Relative Instrument Price Mid Cost
Downloads Spec Sheet
System Overview MiSeq Dx Overview
 
MiSeq FGx
Output Range 0.3-15 Gb
Run Time 4-55 hr
Reads per Run 1-25 million (for research)
Maximum Read Length 2 x 300 bp
Samples per Run N/A
Samples per Run 1-96
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Samples per Run N/A
Relative Price per Sample Mid Cost
Relative Instrument Price Mid Cost
Downloads Spec Sheet
System Overview MiSeq FGx Overview
 
NextSeq*
Output Range 20-120 Gb
Run Time 11-29 hr
Reads per Run 130-400 million
Maximum Read Length 2 x 150 bp
Samples per Run 1
Samples per Run 96
Samples per Run 8
Samples per Run 12-36
Samples per Run 1-384
Samples per Run 2-8
Samples per Run 25-80
Samples per Run 8-24
Samples per Run 1
Samples per Run 1-384
Relative Price per Sample Highest Cost
Relative Instrument Price Lowest Cost
Downloads Spec Sheet
System Overview NextSeq Overview
 
HiSeq 4000*
Output Range 125-1500 Gb
Run Time <1-3.5 days
Reads per Run 2.5-5 billion
Maximum Read Length 2 x 150 bp
Samples per Run 6-12
Samples per Run 768-1536
Samples per Run 50-100
Samples per Run N/A
Samples per Run 1-6144
Samples per Run 50-100
Samples per Run 60-792
Samples per Run 160-320
Samples per Run 3-6
Samples per Run N/A
Relative Price per Sample Lower Cost
Relative Instrument Price High Cost
Downloads Spec Sheet
System Overview HiSeq Overview
 
NovaSeq 6000*††
Output Range 134-6000 Gb
Run Time 19-40 hr
Run Time 13-44 hr
Run Time 13-44 hr
Run Time 13-44 hr
Run Time 13-44 hr
Run Time 24-44 hr
Reads per Run Up to 20 billion
Maximum Read Length 2 x 150 bp
Samples per Run 4-48
Samples per Run N/A
Samples per Run 26-750
Samples per Run N/A
Samples per Run N/A
Samples per Run 26-400
Samples per Run N/A
Samples per Run 86-768
Samples per Run 2-30
Samples per Run N/A
Relative Price per Sample Lowest Cost
Relative Instrument Price Higher Cost
Downloads Spec Sheet
System Overview NovaSeq Overview


  • For Research Use Only. Not for use in diagnostic procedures.
  • For In Vitro Diagnostic applications.
  • Samples per run assume capability to address individual lanes of the NovaSeq flow cell. They are based on 30X coverage of whole human genome at ≥ 100Gb per genome. Sample number will vary for other species based on relative genome size.
  • Based on 30X or greater coverage of a 5Mb microbial genome. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
  • Samples per run assume capability to address individual lanes of the NovaSeq flow cell. Assumes ~8Gb per sample; 200 cycle kit for S1 & S2 and 300 cycle kit for S4. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
  • Based on TruSight One Sequencing Panel. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
  • 10 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
  • Samples per run assume capability to address individual lanes of the NovaSeq flow cell. Based on ≥ 50M reads at 2×50 bp. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
  • 5 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
  • Samples per run assume capability to address individual lanes of the NovaSeq flow cell. Based on ≥ 15 million reads per sample for identification of transcription factor binding. Additional reads required for histone marks. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
  • Samples per run assume capability to address individual lanes of the NovaSeq flow cell. Based on 200 Gb per sample at 30X coverage. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 2×75 reads or greater.
  • Based on Nextera XT indexing. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
  • Lower limits of output and reads per run ranges based on a single S1 flow cell run; upper limits based on dual S4 flow cell run. Run times include cluster generation, sequencing, and base calling. Run times are based on running two flow cells of the same type. Starting two different flow cells will impact run time. NovaSeq S1 Reagent Kits are not currently available.
  • For Research, Forensic, and Paternity Use Only.