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Next-generation sequencing (NGS) technology can provide a broad view of the human genome, helping us discover and apply relevant genomic information to human health. Ultimately, this may lead to more accurate diagnosis, prognosis, and therapeutic selection. Clinical laboratories can take advantage of the benefits of NGS with the FDA-cleared MiSeqDx system.
At the heart of the system is the FDA-cleared MiSeqDx instrument, stringently validated for design and performance. Two IVD assays and one IVD kit are currently available for use on the MiSeqDx instrument:
The MiSeq FGx Forensic Genomics System is the first fully validated sequencing system designed for use in forensic genomics applications. The complete sample-to-answer system uses dedicated library preparation kits, customized instrument control software, and an analytical software package to support the reliable analysis of both routine and challenging forensic samples.
The MiSeq FGx System workflow is easy-to-use, automated, and fast:
| NovaSeq System | HiSeq X Series* | ||
|---|---|---|---|
| NovaSeq 6000† | HiSeq X Five† | HiSeq X Ten† | |
| Output Range | 167–6000 Gb‡ | 900–1800 Gb | 900–1800 Gb |
| Run Time | 19–40 hr | <3 days | <3 days |
| Reads per Run | 1.4–20 billion§ | 3–6 billion | 3–6 billion |
| Maximum Read Length | 2 × 150 bp | 2 × 150 bp | 2 × 150 bp |
| Samples per Run| | 4–48 | 8–16 | 8–16 |
| Relative Price per Sample| | Higher Cost | Higher Cost | Lower Cost |
| Relative Instrument Price| | Lower Cost | Lower Cost | Higher Cost |
| Downloads | Spec Sheet | Spec Sheet | Spec Sheet |
* Specifications shown for an individual HiSeq X Sequencing System. HiSeq X is only available as part of the HiSeq X Five or HiSeq X Ten.
† For Research Use Only. Not for use in diagnostic procedures.
‡ Output Range is based on a dual flow cell run of S4 Flow Cells which have not been released; therefore, performance metrics are subject to change.
§ Reads Per Run is based on a dual flow cell run of S4 Flow Cells which have not been released; therefore, performance metrics are subject to change.
| Based on 30X coverage of a human genome, assumes minimum of 100Gb per genome. Sample number will vary for other species based on relative genome size.
| MiniSeq System | MiSeq Series | NextSeq System | HiSeq System | NovaSeq Series†† | ||||
|---|---|---|---|---|---|---|---|---|
| MiniSeq | MiSeq* | MiSeqDx† | MiSeq FGx‡ | NextSeq 500* | HiSeq 4000* | NovaSeq 5000* | NovaSeq 6000* | |
| Output Range | 1.8–7.5 Gb | 0.3–15 Gb | 0.3–15 Gb | 0.3–15 Gb | 20–120 Gb | 125–1500 Gb | 167—2000 Gb | 167—6000 Gb |
| Run Time | 4–24 hr | 5–55 hr | 4–55 hr | 4–55 hr | 11–29 hr | <1–3.5 days | TBA | 19—40 hr |
| Reads per Run | 8–25 million | 1–25 million | 1–25 million | 1–25 million (for research) | 130–400 million | 2.5–5 billion | 1.4–6.6 billion | 1.4–20 billion |
| Max Read Length | 2 × 150 bp | 2 × 300 bp | 2 × 300 bp | 2 × 300 bp | 2 × 150 bp | 2 × 150 bp | 2 × 150 bp | 2 × 150 bp |
| Samples per Run§ | N/A | N/A | N/A | N/A | 1 | 6–12 | 4—16 | 4—48 |
| Samples per Run§ | 1–384 | 1–384 | N/A | 1–96 | 96 | 768–1536 | N/A | N/A |
| Samples per Run§ | N/A | N/A | N/A | N/A | 8 | 50—100 | 32—132 | 32—192 |
| Samples per Run§ | 1–96 | 1–96 | N/A | N/A | 12–36 | N/A | N/A | N/A |
| Samples per Run§ | 1–384 | 1–384 | N/A | N/A | 1–384 | 1–6144 | N/A | N/A |
| Samples per Run§ | N/A | N/A | N/A | N/A | 2–8 | 50–100 | 32—132 | 32—192 |
| Samples per Run§ | 1–5 | 1–5 | N/A | N/A | 25–80 | 60–792 | N/A | N/A |
| Samples per Run§ | 1–6 | 1–6 | N/A | N/A | 8–24 | 160–320 | 96—192 | 96—192 |
| Samples per Run§ | N/A | N/A | N/A | N/A | 1 | 3–6 | 2—8 | 2–24 |
| Samples per Run§ | 1–384 | 1–384 | N/A | N/A | 1–384 | N/A | N/A | N/A |
| Relative Price per Sample§ | Higher Cost | Higher Cost | Higher Cost | Higher Cost | Mid Cost | Lower Cost | Lower Cost | Lower Cost |
| Relative Instrument Price§ | Lower Cost | Lower Cost | Lower Cost | Lower Cost | Mid Cost | Higher Cost | Higher Cost | Higher Cost |
| Downloads | Spec Sheet | Spec Sheet | Spec Sheet | Spec Sheet | Spec Sheet | Spec Sheet | Spec Sheet | Spec Sheet |
†† All output and read number specifications based on dual flow cell runs. Run time includes cluster generation, sequencing, and base calling for a Dual S2 Flow Cell Run on the NovaSeq 6000 System. Run times on the NovaSeq 5000 System may be slightly longer.
* For Research Use Only. Not for use in diagnostic procedures.
† For In Vitro Diagnostic applications.
‡ For Research, Forensic, and Paternity Use Only.
§ Based on 30X coverage of a human genome, assumes minimum of 100Gb per genome. Sample number will vary for other species based on relative genome size.
§ Based on 30X or greater coverage of a 5Mb microbial genome. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
§ Based on 8 Gb per sample at 2x75 bp. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 50M reads at 2x75bp or greater.
§ Based on TruSight One Sequencing Panel. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
§ 10 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
§ 50 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 50M reads at 2x50bp or greater.
§ 5 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.
§ 15 million reads per sample for identification of transcription factor binding. Additional reads required for histone marks. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 15M reads at 1x50bp or greater.
§ 30X coverage. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 2x75 reads or greater.
