Sequencing platform selector

This tool will help you compare next-generation sequencing systems and find the one that’s right for your lab or application

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Research Use Only Molecular Diagnostics Forensic Genomics

Cancer Genomics Agrigenomics Genetic Disease Microbial Genomics Reproductive Health Population-scale WGS Other Area of Interest

Large Whole-Genome Sequencing (human, plant, animal) Small Whole-Genome Sequencing (microbe, virus) Exome Sequencing Targeted Gene Sequencing (amplicon, gene panel) Targeted RNA Sequencing Whole-Transcriptome Sequencing Small RNA Analysis ChIP-Seq Methylation Sequencing 16S Metagenomic Sequencing

MiSeqDx

MiSeqDx

Next-generation sequencing (NGS) technology can provide a broad view of the human genome, helping us discover and apply relevant genomic information to human health. Ultimately, this may lead to more accurate diagnosis, prognosis, and therapeutic selection. Clinical laboratories can take advantage of the benefits of NGS with the FDA-cleared MiSeqDx system.

At the heart of the system is the FDA-cleared MiSeqDx instrument, stringently validated for design and performance. Two IVD assays and one IVD kit are currently available for use on the MiSeqDx instrument:

  • The MiSeqDx Universal Kit allows users to develop their own NGS laboratory tests, expanding their lab offerings to meet ever-evolving needs
  • The MiSeqDx Cystic Fibrosis 139-Variant Assay detects 139 clinically relevant and functionally verified CFTR variants
  • The MiSeqDx Cystic Fibrosis Clinical Sequencing Assay detects mutations within the protein coding regions and intron/exon boundaries of CFTR

MiSeq FGx

MiSeq FGx

The MiSeq FGx Forensic Genomics System is the first fully validated sequencing system designed for use in forensic genomics applications. The complete sample-to-answer system uses dedicated library preparation kits, customized instrument control software, and an analytical software package to support the reliable analysis of both routine and challenging forensic samples.

The MiSeq FGx System workflow is easy-to-use, automated, and fast:

  • Compact, all-in-one platform incorporates cluster generation, paired-end fluidics, and SBS chemistry
  • Intuitive touch screen interface makes for simple instrument operation
  • Plug-and-play reagents with RFID tracking make for added speed and convenience
  NovaSeq System HiSeq X Series*
  NovaSeq 6000 HiSeq X Five HiSeq X Ten
Output Range 167–6000 Gb 900–1800 Gb 900–1800 Gb
Run Time 19–40 hr <3 days <3 days
Reads per Run 1.4–20 billion§ 3–6 billion 3–6 billion
Maximum Read Length 2 × 150 bp 2 × 150 bp 2 × 150 bp
Samples per Run| 4–48 8–16 8–16
Relative Price per Sample| Higher Cost Higher Cost Lower Cost
Relative Instrument Price| Lower Cost Lower Cost Higher Cost
Downloads Spec Sheet Spec Sheet Spec Sheet

* Specifications shown for an individual HiSeq X Sequencing System. HiSeq X is only available as part of the HiSeq X Five or HiSeq X Ten.

† For Research Use Only. Not for use in diagnostic procedures.

‡ Output Range is based on a dual flow cell run of S4 Flow Cells which have not been released; therefore, performance metrics are subject to change.

§ Reads Per Run is based on a dual flow cell run of S4 Flow Cells which have not been released; therefore, performance metrics are subject to change.

| Based on 30X coverage of a human genome, assumes minimum of 100Gb per genome. Sample number will vary for other species based on relative genome size.

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  MiniSeq System MiSeq Series NextSeq System HiSeq System NovaSeq Series††
  MiniSeq MiSeq* MiSeqDx MiSeq FGx NextSeq 500* HiSeq 4000* NovaSeq 5000* NovaSeq 6000*
Output Range 1.8–7.5 Gb 0.3–15 Gb 0.3–15 Gb 0.3–15 Gb 20–120 Gb 125–1500 Gb 167—2000 Gb 167—6000 Gb
Run Time 4–24 hr 5–55 hr 4–55 hr 4–55 hr 11–29 hr <1–3.5 days TBA 19—40 hr
Reads per Run 8–25 million 1–25 million 1–25 million 1–25 million (for research) 130–400 million 2.5–5 billion 1.4–6.6 billion 1.4–20 billion
Max Read Length 2 × 150 bp 2 × 300 bp 2 × 300 bp 2 × 300 bp 2 × 150 bp 2 × 150 bp 2 × 150 bp 2 × 150 bp
Samples per Run§ N/A N/A N/A N/A 1 6–12 4—16 4—48
Samples per Run§ 1–384 1–384 N/A 1–96 96 768–1536 N/A N/A
Samples per Run§ N/A N/A N/A N/A 8 50—100 32—132 32—192
Samples per Run§ 1–96 1–96 N/A N/A 12–36 N/A N/A N/A
Samples per Run§ 1–384 1–384 N/A N/A 1–384 1–6144 N/A N/A
Samples per Run§ N/A N/A N/A N/A 2–8 50–100 32—132 32—192
Samples per Run§ 1–5 1–5 N/A N/A 25–80 60–792 N/A N/A
Samples per Run§ 1–6 1–6 N/A N/A 8–24 160–320 96—192 96—192
Samples per Run§ N/A N/A N/A N/A 1 3–6 2—8 2–24
Samples per Run§ 1–384 1–384 N/A N/A 1–384 N/A N/A N/A
Relative Price per Sample§ Higher Cost Higher Cost Higher Cost Higher Cost Mid Cost Lower Cost Lower Cost Lower Cost
Relative Instrument Price§ Lower Cost Lower Cost Lower Cost Lower Cost Mid Cost Higher Cost Higher Cost Higher Cost
Downloads Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet

†† All output and read number specifications based on dual flow cell runs. Run time includes cluster generation, sequencing, and base calling for a Dual S2 Flow Cell Run on the NovaSeq 6000 System. Run times on the NovaSeq 5000 System may be slightly longer.

* For Research Use Only. Not for use in diagnostic procedures.

† For In Vitro Diagnostic applications.

‡ For Research, Forensic, and Paternity Use Only.

§ Based on 30X coverage of a human genome, assumes minimum of 100Gb per genome. Sample number will vary for other species based on relative genome size.

§ Based on 30X or greater coverage of a 5Mb microbial genome. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ Based on 8 Gb per sample at 2x75 bp. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 50M reads at 2x75bp or greater.

§ Based on TruSight One Sequencing Panel. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 10 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 50 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 50M reads at 2x50bp or greater.

§ 5 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 15 million reads per sample for identification of transcription factor binding. Additional reads required for histone marks. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 15M reads at 1x50bp or greater.

§ 30X coverage. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results. Based on 2x75 reads or greater.

§ Based on Nextera XT indexing. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.



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