The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports a broad range of applications and study sizes. Choose between rapid run and high output modes to fit your project needs.
Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 250 base pair reads. The longer reads enable greater depth of coverage, improved assembly for de novo applications and aid in long-read applications such as metagenomic analyses.
High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process over 6× more samples than rapid mode and to complete large projects quickly and efficiently. HiSeq SBS Kits v4 produce 4 billion clusters per run, generating up to 1 Tb of data in 6 days.
Learn how DNA sequencing is leading to progress in the rapid identification of variants that might be involved in genetic diseases. Thanks to this work, Dr. Kingsmore and his team now hold a Guinness World Records title.View Video
Using the HiSeq 2500 System, Dr. Hector Alvarez and researchers at the MD Anderson Cancer Center are studying genetic variation in exosomal DNA found in the blood of pancreatic cancer subjects and gaining insight into the potential of liquid biopsies in diagnosing and monitoring the disease.Read Article
Led by Carola Vinuesa, MD, PhD, researchers at Australian National University are performing human whole-genome sequencing and whole-exome sequencing on HiSeq X Ten and HiSeq 2500 Systems to identify variants associated with lupus and other autoimmune disease.Read Article
The cBot 2 System streamlines the sequencing workflow by automating cluster generation. The cBot 2 System, a required accessory instrument for the HiSeq 2500 System, creates clonal clusters from single molecule DNA templates, preparing them for sequencing by synthesis.Learn More
This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.
HiSeq Rapid SBS Kits v2 are designed for the rapid run mode of HiSeq 2500 and 1500 sequencers.
Reagents for determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis technology on enabled HiSeq systems.