HiSeq 3000/HiSeq 4000 resources to further your research

System literature, customer stories, and webinars to support production-scale genomics

HiSeq 3000/HiSeq 4000 Resources

Find tools to help you choose the right products for your needs, and plan your experiments.

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Product Selection and Experiment Planning Tools
HiSeq 3000 User Guide

Describes instrument components, on-instrument software, the sequencing workflow, and maintenance procedures for the HiSeq 4000.

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Sequencing Online Training

Online Illumina sequencing courses are free, interactive, and available any time.

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Personalized Training Workflow Selection Tool

This tool will help you determine the best hands-on sequencing training for your needs based on your area of interest, application, library preparation kit, and platform.

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HiSeq 4000 User Guide

Describes instrument components, on-instrument software, the sequencing workflow, and maintenance procedures for the HiSeq 4000.

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Sequencing Coverage

Sequencing coverage describes the average number of NGS reads that align to known reference bases. Coverage requirements vary by application.

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Quality Scores

Additional information about sequencing quality scores, how they are calculated, and the relationship between quality score and base call accuracy.

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HiSeq 3000/HiSeq 4000 Site Prep Guide

The site prep guide contains lab specifications and requirements for preparing the lab for sequencing on the HiSeq 3000 or HiSeq 4000 System.

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Sequencing Troubleshooting Tips

These short videos provide expert tips for common sequencing issues such as overclustering and inconsistent quantitation.

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Custom Protocol Selector

The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

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HiSeq 3000 Support Documentation

Access guides, literature, and technical notes for the HiSeq 3000 System.

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HiSeq 4000 Support Documentation

Access guides, literature, and technical notes for the HiSeq 4000 System.

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Progress in Rapid Identification of Variants Linked to Genetic Disease
Progress in Rapid Identification of Variants Linked to Genetic Disease

Learn how DNA sequencing is leading to progress in the rapid identification of variants that might be involved in genetic diseases. Thanks to this work, Dr. Kingsmore and his team now hold a Guinness World Records title.

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Streamline NGS Data Analysis, Management, and Storage

BaseSpace Sequence Hub increases an Oxford laboratory's productivity and cost-effectiveness in meeting the needs of its clinical research teams.

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Exploring the Forgotten Genome of Cancer Research

A Garvan Institute sarcoma study identifies rare cancer-associated variants in the germline with the HiSeq 2500 System.

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Illumina High Throughput Sequencing Portfolio Video
Illumina High Throughput Sequencing Portfolio Video

Increase your throughput and coverage depth with the increased capacity of the HiSeq 3000 and HiSeq 4000 Systems.

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Leveraging Patterned Flow Cell Technology
Leveraging Patterned Flow Cell Technology

Learn how to harness the power of the HiSeq 3000 and HiSeq 4000 Systems for your diverse high-throughput sequencing applications.

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Human Genetics and Cancer Genomics
Human Genetics and Cancer Genomics

Discover how linked-reads and phased genome analysis provide insights in human genetics and oncology.

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Exome Sequencing on the HiSeq 4000 System
Exome Sequencing on the HiSeq 4000 System

Demonstration of high-throughput NGS methods on the HiSeq 4000 instrument, focusing on the exome sequencing workflow.

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Core Facility Case Study
Core Facility Case Study

Learn how the UCSF Center for Advanced Technology transitioned from the HiSeq 2500 System to the HiSeq 4000 System.

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