TruSeq RNA Access Library Prep Kit

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA. Read More...
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TruSeq RNA Access Library Prep Kit - Set A (12 indexes, 48 samples)

RS-301-2001

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TruSeq RNA Access Library Prep Kit - Set B (12 indexes, 48 samples)

RS-301-2002

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Product Highlights

TruSeq RNA Access kits convert total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from FFPE (formalin-fixed, paraffin-embedded) tissues and other low-quality samples.

  • Affordability and focus - isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth
  • High-quality data from difficult samples - optimized for sequencing RNA from degraded samples, including FFPE tissues
  • Samples with limited starting material - greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity

TruSeq RNA Access Kit generates RNA-Seq libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions maximizes discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit

Specifications

Method-Specific Workflow Example

 

Supporting Data and Figures

Efficient Gene Fusion Discovery
Supporting Data and Figures

Figure 5 from TruSeq RNA Access Library Prep Kit Data Sheet, March 2014

The TruSeq RNA Access Library Prep Kit enables detection of expressed fusion transcripts without the need to design probes specific for the fusion junction. The well-characterized BCR-ABL fusion is detected efficiently in the Universal Human Reference RNA (UHRR) sample at 25 M reads.

 

Manuals and Support Information

TruSeq RNA Access Library Prep Kit Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq RNA Access Library Prep Kit Support 

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