The product previously known as the TruSeq RNA Access Library Prep Kit (Cat. No. RS-301-2001 and RS-301-2002) is now called TruSeq RNA Exome. The product configuration has changed. In the new configuration, major components such as library preparation, index adapters, enrichment reagents, and exome panels can be purchased separately. If you were previously buying Cat. No. RS-301-2001 or Cat. No. RS-301-2002, purchase the following components: TruSeq RNA Library Prep for Enrichment (Cat. No. 20020189), TruSeq RNA Enrichment (Cat. No. 20020490), Exome Panel (Cat. No. 20020183), and one item from the Index Adapters section below.

TruSeq RNA Exome

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.Read More...
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Library Prep

TruSeq® RNA Library Prep for Enrichment (48 Samples)




TruSeq® RNA Enrichment (12 enrichments)



Index Adapters

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)



TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)




Exome Panel (45Mb)


Accessory Products

Illumina Free Adapter Blocking Reagent (12 reactions)



Illumina Free Adapter Blocking Reagent (48 reactions)



Product Highlights

TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.

  • Affordability and focus—Isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth
  • High-quality data from difficult samples—Optimized for sequencing RNA from degraded samples, including FFPE tissues
  • Samples with limited starting material—Greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity

TruSeq RNA Exome generates RNA sequencing (RNA-Seq) libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions to help maximize discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis. Reagent volumes supplied are sufficient to support 1- to 4-plex enrichment reactions.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this workflow


Method-Specific Workflow Example


Customer Stories

Looking Beyond Single-Drug Approaches to Precision Oncology Research

Researchers rely on transcriptomics to uncover multiple drug-susceptible tumorigenic pathways.

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A Tale of Two RNA Library Prep Kits

A critical comparison between two popular TruSeq RNA Library Prep Kits reveals new information of interest to researchers conducting RNA sequencing studies.

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Supporting Data and Figures

Efficient Gene Fusion Discovery
Supporting Data and Figures

TruSeq RNA Exome enables detection of expressed fusion transcripts without the need to design probes specific for the fusion junction. The well-characterized BCR-ABL fusion is detected efficiently in the Universal Human Reference RNA (UHRR) sample at 25 M reads.


Manuals and Support Information

TruSeq RNA Exome Support - Documentation & Literature

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq RNA Exome Support 

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