Products for the HiSeq 3000 and HiSeq 4000 Systems

Kits, reagents, and accessories for production-scale genomics

HiSeq 3000/HiSeq 4000 Products

Areas of Research Focus

Product Categories

Key Use

Nucleic Acid Type
Library Preparation Kits
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Nextera DNA Library Preparation Kit

Nextera DNA kits provide a fast and easy library prep workflow, delivering whole-genome sequencing libraries in under 90 min.

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Nextera Mate Pair Library Prep Kit

This library preparation kit offers a gel-free method for preparing up to 12 kb mate pair libraries with the industry’s lowest DNA input requirement.

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Nextera Rapid Capture Custom Enrichment Kit

Custom target enrichment kit to rapidly interrogate those portions of the human genome most important to your specific research.

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Nextera Rapid Capture Exome and Expanded Exome Kits

Identify coding variants up to 70% faster with this exome library prep and enrichment kit. Expanded kit sequences untranslated regions (UTRs) and miRNA.

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SureCell WTA 3′ Library Prep Kit for the ddSEQ System

A robust, scalable, and user-friendly workflow for transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment.

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TruSeq ChIP Library Preparation Kit

Simple, cost-effective chromatin immunoprecipitation sequencing (ChIP-Seq) DNA library preparation, with master mixes and robust multiplex capabilities.

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TruSeq Custom Amplicon Low Input Kit

This library prep kit delivers sensitive and specific amplicon sequencing results from both low-input and FFPE DNA samples.

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TruSeq Custom Amplicon v1.5

Focus on key genomic regions of interest with this targeted resequencing solution, useful for very high plexity pools or longer amplicons.

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TruSeq DNA PCR-Free Library Preparation Kit

Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.

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TruSeq Exome

This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.

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TruSeq Methyl Capture EPIC Library Prep Kit

This kit combines next-generation sequencing with epigenetic insights to accelerate biomarker discovery and understand methylation’s role in gene regulation.

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TruSeq Nano DNA Library Prep Kit

Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.

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TruSeq Nano DNA Library Prep Kit for NeoPrep

DNA library preparation for use with the NeoPrep System, providing comprehensive genomic coverage with low DNA input and minimal hands-on time.

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TruSeq RNA Access Library Prep Kit

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.

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TruSeq RNA Library Prep Kit v2

The TruSeq RNA Library Preparation Kits provide a simple, cost-effective solution for analysis of the coding transcriptome.

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TruSeq Rapid Exome Library Prep Kit

This exome sequencing library prep kit uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome.

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TruSeq Stranded Total RNA Library Prep Kit

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and FFPE.

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TruSeq Stranded Total RNA with Ribo-Zero Globin

Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes rRNA and globin mRNA in a single step.

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TruSeq Stranded mRNA Library Prep Kit

Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.

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TruSeq Stranded mRNA Library Prep Kit for NeoPrep

Complete view of the transcriptome with walk-away workflow, lower input requirement, and excellent reproducibility.

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TruSeq Synthetic Long-Read DNA Library Prep Kit

Use this sequencing kit to prepare DNA libraries to generate synthetically long reads, with informatics options for genome assembly or genome phasing.

Cluster Generation and Sequencing Reagents
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HiSeq 3000/4000 PE Cluster Kit

This kit enables cluster generation for subsequent sequencing on the HiSeq 3000 and HiSeq 4000 Systems.

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HiSeq 3000/4000 SBS Kit

Includes sequencing reagents specifically designed for the HiSeq 3000/HiSeq 4000 Systems. Enables production-scale sequencing for diverse applications.

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HiSeq 3000/4000 SR Cluster Kit

Provides reagents for Single Read (SR) cluster generation on the cBot2 System. Cluster reagents are compatible with the HiSeq 3000 and HiSeq 4000 Systems.

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PhiX Control v3

PhiX Control v3 is a ready-to-use control library for Illumina sequencing runs.

Informatics Products
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BaseSpace Clarity LIMS

BaseSpace Clarity LIMS is a laboratory information management system that helps laboratories track samples and optimize procedures and workflows.

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BaseSpace Cohort Analyzer

BaseSpace Cohort Analyzer allows you to integrate and analyze subject and genomic data together using innovative visualization and analysis tools.

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BaseSpace Correlation Engine

BaseSpace Correlation Engine mines over 20,000 genomic studies to get data-driven answers for genes, experiments, drugs and phenotypes for your research.

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BaseSpace Sequence Hub

Simplify your bioinformatics with an economical and powerful computing environment to manage, analyze, and share data.

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BaseSpace Variant Interpreter (Beta)

BaseSpace Variant Interpreter (Beta) enables genetic testing labs to rapidly annotate, filter, and interpret genomic data.

Molecular Biology Reagents
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Globin-Zero Gold rRNA Removal Kit

This kit removes unwanted ribosomal RNA and globin mRNA from mammalian blood RNA samples before sequencing.

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Ribo-Zero Gold rRNA Removal Kit (Epidemiology)

This kit removes cytoplasmic and mitochondrial rRNA from samples composed of human/mouse/rat, Gram-positive and Gram-negative bacterial RNA.

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Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat)

This kit removes both cytoplasmic and mitochondrial rRNA from human, mouse, and rat samples, including partially degraded samples.

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Ribo-Zero Gold rRNA Removal Kit (Yeast)

This kit removes both cytoplasmic and mitochondrial ribosomal RNA from yeast samples.

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Ribo-Zero rRNA Removal Kit (Bacteria)

This kit removes ribosomal RNA from Gram-positive and Gram-negative bacterial mixtures in a single pass.

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Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria)

This kit removes unwanted ribosomal RNA from Gram-negative bacterial samples.

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Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria)

This kit removes unwanted ribosomal RNA from Gram-positive bacterial samples.

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Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat)

This kit removes cytoplasmic ribosomal RNA from human, mouse, or rat samples, including partially degraded samples.

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Ribo-Zero rRNA Removal Kit (Plant)

This kit removes cytoplasmic, mitochondrial, and chloroplast ribosomal RNA from plant seed, root, or leaf samples in a single pass.

Accessory Products
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cBot 2 Barcoded Strip Tubes

These tubes enable sequencing sample tracking on the cBot 2 automated cluster generation system.

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cBot 2 System

The cBot 2 System streamlines the sequencing workflow by automating cluster generation. It is a required accessory instrument for HiSeq sequencers.