HT1 Hybridization Buffer

Reagent used to dilute denatured libraries and the PhiX control library prior to sequencing.

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Overview
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Overview

The HT1 Hybridization Buffer is an Illumina reagent designed for diluting denatured libraries and the PhiX control library to ensure the proper loading concentration for binding to the flow cells. In addition to diluting denatured libraries, the buffer is also used to dilute custom primers before adding to the cluster cartridge.

Specifications

Instruments	MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System, NextSeq 500 System, NovaSeq 6000 System
Technology	Sequencing

Documentation

Product literature

Literature is not currently available for this product.

Support documentation

NextSeq System Denature and Dilute Libraries Guide

NextSeq 500 and 550 Systems Denature and Dilute Libraries Guide

NovaSeq 6000 Denature and Dilute Libraries Guide

MiniSeq System Denature and Dilute Libraries Guide

MiSeq System: Denature and Dilute Libraries Guide

Resources

MiSeq: How to denature and dilute libraries and spike in PhiX

HT1 Buffer

20015892

Includes one, 5-ml tube of reagent for diluting denatured libraries and the PhiX control library prior to sequencing.

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HT1 Hybridization Buffer

Overview

Specifications

Documentation

Product literature

Support documentation

Resources

Selection summary

NovaSeq X Series ordering

MiSeq Reagent Kit v3

TruSight Oncology 500

Illumina DNA Prep

PhiX Control v3

MiSeq Reagent Kit v3

NovaSeq 6000 Reagent Kits

Speak with a specialist