NextSeq 500/550 v2 kits have been discontinued. In addition, NextSeq TG reagents v1, v1.2, and v2 will be discontinued on October 31, 2019, and the NextSeq TG v2.1 reagent discontinuation date is July 31, 2019. With increased stability, the NextSeq 500/550 v2.5 Kits are the recommended replacement.

NextSeq 500/550 TG Kits - v1.2, v2, and v2.1

These sequencing reagent kits offer a simplified workflow and improved data quality compared to the prior v1 kit version.Read More...
Select Product(s)

TG NextSeq® 500/550 High Output Kit v2 (75 cycles)



TG NextSeq® 500/550 High Output Kit v2 (150 cycles)



TG NextSeq® 500/550 High Output Kit v2 (300 cycles)



TG NextSeq 500/550 High Output Kit v2.1 (75 cycles)



TG NextSeq 500/550 High Output Kit v2.1 (150 cycles)



TG NextSeq 500/550 High Output Kit v2.1 (300 cycles)



TG NextSeq® 500/550 Mid Output Kit v2 (150 cycles)



TG NextSeq® 500/550 Mid Output Kit v2 (300 cycles)



TG NextSeq® 500/550 High Output Kit v1.2 (75 cycles)


TG NextSeq® 500 High Output Kit v1 (75 cycles)



Product Highlights

TG NextSeq 500/550 v2 sequencing reagent kits bring the power of a high-throughput sequencing system to the benchtop, now with improved data quality and the highest yield of error-free reads. These kits offer:

  • Robust base calling and improved signal-to-noise ratio
  • Multiple options for both sequencing output and read length
  • Simplified reagent cartridge configurations, with improved workflow and cartridge loading
  • Intuitive labeling and RFID-encoded reagents
  • Optimized chemistry with the latest in sequencing by synthesis (SBS) and bridge-amplification cluster generation

The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Ready-to-use cartridges redefine ease-of-use
Pre-configured reagent kits for the NextSeq Series Systems provide all the components needed for sequencing on the platform in 3 ready-to-use cartridges (reagent, flow cell, and buffer). The kits offer simplified cartridge loading with the bleach and dual-indexing primers already in the cartridge.

Simply load user-prepared library pools directly into the NextSeq reagent cartridge. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

This product is available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

TG NextSeq 500/550 v2 and v2.1 kits use the same reagents and consumables, but contain different flow cells. Contact your local Illumina representative to determine which flow cell best meets your specific needs.

Frequently Purchased Together


Best Practices for Storing Sequencing Reagents

Sequencing reagent performance depends on thawing technique, thawed storage time, and storage conditions. Learn how to thaw and store sequencing reagents for optimal performance.

View Bulletin
Best Practices for Storing Sequencing Reagents

Configurations for every application

Researchers have a wide range of solutions for different applications, from ChIP-Seq and RNA profiling to whole-genome sequencing, as shown in the table. NextSeq reagent kits provide optimized chemistry with paired-end sequencing capability built into every reagent kit.


NextSeq 500/550 Kits High Output
Up to 400 million clusters
Mid Output
Up to 130 million clusters

300 cycles
  • Whole Genome
  • de novo
  • Exome
  • Targeted Panels
  • Exome

150 cycles
  • Whole Transcriptome
  • mRNA-Seq
  • Exome
  • Whole Transcriptome
  • mRNA-Seq
  • Exome

75 cycles


  • Gene Expression Profiling
  • NIPT

†All NextSeq 500/550 kits are paired-end capable

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Product Literature

NextSeq 550 Sequencing System

Data Sheet | PDF2 MB

Manuals and Support Information

NextSeq 500/550 Kit Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

All NextSeq 500/550 Kit Support 

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