This kit is compatible with unique dual index kits (available soon), which allow multiplexing of up to 96 samples.

TruSeq Stranded mRNA Library Prep Kit

Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information. Read More...
Select Product(s)

TruSeq Stranded mRNA Library Preparation Kit Set A (48 samples, 12 indexes)



TruSeq Stranded mRNA Library Preparation Kit Set B (48 samples, 12 indexes)



TruSeq Stranded mRNA Library Prep Kit High Throughput (96 samples, 96 indexes)



Product Highlights

The TruSeq Stranded mRNA Library Prep Kit offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It is compatible with a wide range of samples.

Precise and Accurate

Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.


Stranded information identifies from which of the 2 DNA strands a given RNA transcript was derived. This information provides increased confidence in transcript annotation, particularly for nonhuman samples. Identifying strand origin increases the percentage of reads that align, reducing sequencing costs per sample.


The kit enables robust interrogation of both standard and low-quality samples, and workflows compatible with a wide range of study designs.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit


Method-Specific Workflow Example


Customer Stories

Unraveling Disease Mechanisms With The NextSeq 500 System

Researchers at the Icahn School of Medicine at Mount Sinai are studying mtDNA, piRNA, and TCR repertoire diversity to unlock the secrets of cancer, infertility, and autoimmune disease.

Read More
BRC-Seq Customers Benefit from Streamlined BaseSpace Integrated Workflow

BaseSpace enables tracking of samples, from library prep to data analysis, helping BRC-Seq deliver high-quality sequencing data and additional value to its customers.

Read More

Supporting Data and Figures

Technical Replicates
Supporting Data and Figures

Figure 2 from TruSeq Stranded mRNA and Total RNA Library Prep Kits Data Sheet, December 2015

Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.

Other Kit Configurations

TruSeq Stranded mRNA Library Prep is also available in a kit version compatible with the NeoPrep Library Prep System, our solution for automated library preparation.

View NeoPrep-Compatible Version of the Kit

Related Products

This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERCIAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.