ScriptSeq Complete Kit (Plant)

These kits remove unwanted ribosomal RNA from plant leaf, seed, and root samples before creating stranded sequencing libraries. Read More...
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ScriptSeq Complete Kit (Plant Leaf) 6 reactions

BPL1205

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ScriptSeq Complete Kit (Plant Leaf) 24 reactions

BPL1224

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ScriptSeq Complete Kit (Plant Seed/Root) 6 reactions

BSR1206

Price
 
 
Accessory Products
What accessories do I need?

ScriptSeq Index PCR Primers (Set 1)

RSBC10948

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ScriptSeq Index PCR Primers (Set 2)

SSIP1202

Price
 
 

ScriptSeq Index PCR Primers (Set 3)

SSIP1203

Price
 
 

ScriptSeq Index PCR Primers (Set 4)

SSIP1204

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ScriptSeq Index PCR Primers (Sets 1-4)

SSIP1234

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Product Highlights

  • Removes ribosomal RNA (rRNA) in a single pass
  • Creates a stranded Illumina sequencing library
  • Sequencing data contains complete transcriptome of coding and non-coding RNA species
  • Good for intact and partially degraded samples
  • Barcode/index-compatible: ScriptSeq index primers available separately for preparing barcoded libraries

Sequencing is an important discovery tool for plant studies. The sequencing data from samples treated with ScriptSeq Complete (Plant Leaf) and ScriptSeq Complete (Plant Seed/Root) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

The ScriptSeq Complete Gold (Plant Leaf) and ScriptSeq Complete (Plant Seed/Root) kits offer the most informative sequencing results by removing unwanted rRNA prior to creation of a stranded sequencing library. In order to achieve maximal depletion of rRNA, ScriptSeq Complete (Plant Leaf) contains a higher percentage of probes for removal of chloroplast rRNA compared to ScriptSeq Complete (Seed/Root). When performing stranded analysis on samples prepared with ScriptSeq Complete Gold (Plant Leaf) and ScriptSeq Complete (Plant Seed/Root), choose the FR=secondstrand option.

Related Information

Specifications

Supporting Data and Figures

 

rRNA Types Removed

Plant rRNA Removed
Cytoplasmic Chloroplast Mitochondrial
25S 18S 5.8S 5S 23S 16S 5S 4.5S 18S 5S
The ScriptSeq Complete Kit (Plant Leaf) and ScriptSeq Complete Kit (Plant Seed/Root) remove rRNA from cytoplasmic, chloroplast, and mitochondrial regions. ScriptSeq Complete (Plant Leaf) contains a higher percentage of chloroplast removal probes (compared to seed/root). ScriptSeq Complete (Plant Seed/Root) contains a higher percentage of cytoplasmic rRNA removal probes (compared to plant leaf).

 

Product Literature

Literature is not currently available for this product.

The low input versions of this kit (catalog numbers SCL6PL and SCL6SR) have been discontinued. The standard kit versions can process low input RNA with a minor protocol modification.