5 μg leaf samples of Arabidopsis or Zea mays RNA were depleted of rRNA. The samples were then converted into stranded sequencing libraries with ScriptSeq Complete (Plant Leaf), and sequenced on an Illumina sequencing system. Most reads contain biologically informative content.
The ScriptSeq Complete Kits combine Ribo-Zero rRNA removal reagents with the ScriptSeq v2 RNA-Seq Library Preparation kit. Directional, cluster-ready RNA-Seq libraries - virtually free of rRNA - are prepared in less than 1 day from as little as 100 ng of intact or partially degraded total RNA.
The low input versions of this kit (catalog numbers SCL6PL and SCL6SR) have been discontinued. The standard kit versions can process low input RNA with a minor protocol modification.