Easily go between DNA and RNA projects

With a low purchase price and cost-efficiency even for low sample numbers, MiniSeq makes NGS accessible to every lab

MiniSeq Applications and Methods

Targeted DNA sequencing enables researchers to focus on a select set of genes or relevant gene regions, enabling cost-effective and systematic variant detection. Next-generation sequencing (NGS) can be used to sequence many samples concurrently, increasing both the number of genes covered and the confidence in covering relevant genes.

Targeted resequencing on the MiniSeq System provides highly accurate and reproducible data with a streamlined workflow. The MiniSeq System supports various NGS study designs, including fixed panels of expert-selected content and user-defined, custom gene panels using TruSeq Custom Amplicon.

Estimated Cost Per Sample: $139*

*Targeted resequencing on the MiniSeq System estimated cost per sample based on 750 amplicons, 1000X coverage, 2x150 bp read length, TruSeq Custom Amplicon Low Input Kit, MiniSeq Reagent High Output 300-cycle kit

Small genome sequencing provides comprehensive analysis of microbial or viral genomes for public health, epidemiology, and disease studies. Without requiring bacterial culture, researchers can sequence thousands of small organisms in parallel.

Unlike traditional approaches, small genome sequencing studies using NGS do not rely on labor-intensive cloning steps. Small genome DNA libraries can be prepared, sequenced, and analyzed in as little as 2 days. Sequence up to 50 small genomes per MiniSeq run.

Estimated Cost Per Sample: $64*

*Small whole-genome sequencing on the MiniSeq System estimated cost per sample based on 5 Mb genome, 30x coverage, 2 x 150 bp read length, Nextera XT Library Prep Kit, MiniSeq Reagent High Output 300-cycle kit

Gene expression profiling using our MiniSeq targeted RNA sequencing (RNA-Seq) solution enables measurement of dozens to thousands of targets simultaneously. The MiniSeq System supports user-defined panels to target genes, isoforms, splice junctions, cSNPs, and fusion genes

Predefined panels of expert-selected content target major signal transduction pathways and cellular processes. Sequence 1 to 384 targeted RNA samples per MiniSeq run.

Estimated Cost Per Sample: $23*

*Targeted RNA sequencing on the MiniSeq System estimated cost per sample based on 65 targets, 1000x coverage, 1 x 50 bp read length, TruSeq Targeted RNA Expression custom panel kit, MiniSeq Reagent High Output 75-cycle kit

Tumor Profiling

Focused gene panels to target known mutations in cancer-related genes enable cost-effective tumor profiling and easier data analysis.

Application Note

Tumor Profiling – Solid and Hematological Malignancies

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Germline Mutation Analysis

Germline mutations play an important role in cancer risk and susceptibility. Targeted sequencing enables accurate identification of cancer-predisposing variants.

Application Note

Germline Variant Studies

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Small RNA Discovery and Profiling

Isolate and sequence small RNA species, such as microRNA. Study the role of noncoding RNA in gene silencing and posttranscriptional regulation.

Application Note

miRNA Discovery and Profiling

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16S Metagenomic Sequencing

A culture-free method to identify and compare bacteria from complex microbiomes or environments that are difficult to study.

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Genotyping by Sequencing

Genotyping by sequencing provides a low-cost genetic screening method to discover novel plant and animal SNPs and perform genotyping studies.

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Illumina Methods Guide

Methods Guide

Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications.

Access Guide