AmpliSeq for Illumina RNA Library Prep Training
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Use AmpliSeq for Illumina Custom RNA Panels to measure gene expression in 12 to 1200 gene targets in a single assay. You can easily design custom targeted panels from a menu of over 20,000 human RefSeq genes using DesignStudio Software, a web-based assay design software tool. Key features include:
An AmpliSeq for Illumina Custom RNA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.
Learn More About Ampliseq for IlluminaAmpliSeq for Illumina Custom RNA Panel | TruSeq Targeted RNA Expression Library Prep Kits | |
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Assay Time | 5.5-7.5 hours | 1 day |
Hands-On Time | <1.5 hours | 4 hours |
Input Quantity | 1 ng | 50 ng RNA, 50 ng high-quality total RNA, ≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality |
Mechanism of Action | Multiplex PCR | Amplification |
Method | Amplicon Sequencing, Custom Sequencing, Targeted RNA Sequencing | Targeted RNA Sequencing |
Multiplexing | Up to 384-plex | Up to 384 samples per sequencing run |
Nucleic Acid Type | RNA | RNA |
Specialized Sample Types | Blood, FFPE Tissue | FFPE Tissue, Low-Input Samples |
Species Category | Human | Human, Mouse, Rat |
System Compatibility | iSeq 100, MiniSeq, MiSeq, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550 | MiniSeq, MiSeq, NextSeq 2000, NextSeq 500, NextSeq 550 |
Variant Class | Transcript Variants | Gene Fusions, Single Nucleotide Polymorphisms (SNPs), Transcript Variants |
Application Note | HTML
Moving from qPCR to Custom RNA-Seq with AmpliSeq™ for Illumina Custom RNA Panels Application Note
Application Note | HTML
AmpliSeq for Illumina Custom RNA Sequencing Panel
Data Sheet | HTML
AmpliSeq for Illumina Custom and Community Panels Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All AmpliSeq for Illumina Custom and Community Panels Support