AmpliSeq for Illumina RNA Library Prep Training
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Use AmpliSeq for Illumina Custom RNA Panels to measure gene expression in 12 to 1200 gene targets in a single assay. You can easily design custom targeted panels from a menu of over 20,000 human RefSeq genes using DesignStudio Software, a web-based assay design software tool. Key features include:
An AmpliSeq for Illumina Custom RNA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.
Learn More About Ampliseq for Illumina| AmpliSeq for Illumina Custom RNA Panel | TruSeq Targeted RNA Expression Library Prep Kits | |
|---|---|---|
| Assay Time | 5.5-7.5 hours | 1 day |
| Hands-On Time | <1.5 hours | 4 hours |
| Input Quantity | 1 ng | 50 ng RNA, 50 ng high-quality total RNA, ≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality |
| Mechanism of Action | Multiplex PCR | Amplification |
| Method | Amplicon Sequencing, Custom Sequencing, Targeted RNA Sequencing | Targeted RNA Sequencing |
| Multiplexing | Up to 384-plex | Up to 384 samples per sequencing run |
| Nucleic Acid Type | RNA | RNA |
| Specialized Sample Types | Blood, FFPE Tissue | FFPE Tissue, Low-Input Samples |
| Species Category | Human | Human, Mouse, Rat |
| System Compatibility | iSeq 100, MiniSeq, MiSeq, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550 | MiniSeq, MiSeq, NextSeq 2000, NextSeq 500, NextSeq 550 |
| Variant Class | Transcript Variants | Gene Fusions, Single Nucleotide Polymorphisms (SNPs), Transcript Variants |
Libraries were prepared from 10 ng of Universal Human RNA using an AmpliSeq for Illumina Custom RNA Panel (208 amplicon panel size) and sequenced on the MiSeq System. Results show a high concordance (R2 = 0.997) between the two replicates of the same RNA sample. R2 is a statistical measurement of data correlation.
The AmpliSeq for Illumina Custom RNA Panel enables researches to analyze differential gene expression across genes of interest. To demonstrate assay performance, Human Brain Reference RNA and Universal Human RNA were analyzed using 5 AmpliSeq for Illumina Custom RNA panels of varying size (number of amplicons). Libraries were sequenced on a MiSeq System.
Application Note | HTML
Moving from qPCR to Custom RNA-Seq with AmpliSeq™ for Illumina Custom RNA Panels Application Note
Application Note | HTML
AmpliSeq for Illumina Custom RNA Sequencing Panel
Data Sheet | HTML
AmpliSeq for Illumina Custom and Community Panels Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All AmpliSeq for Illumina Custom and Community Panels Support
Targeted DNA and RNA research panel investigating variants across 161 genes associated with a range of cancer types.
Targeted research panel investigating somatic and germline variants in BRCA1 and BRCA2.
Targeted research panel investigating hotspot regions of 50 genes with known associations to cancer.
Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.
Targeted panel that measures expression levels of >20,000 human RefSeq genes.
MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.
