Targeted, custom RNA research panels optimized for sequencing up to 1200 genomic targets of interest.
Assay time
Hands-on time
Input quantity
Use AmpliSeq for Illumina Custom RNA Panels to measure gene expression in 12 to 1200 gene targets in a single assay. You can easily design custom targeted panels from a menu of over 20,000 human RefSeq genes using DesignStudio Software, a web-based assay design software tool.
An AmpliSeq for Illumina Custom RNA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.
| Assay time | 5.5-7.5 hours |
|---|---|
| Hands-on time | < 1.5 hr |
| Input quantity | 1 ng |
| Instruments | MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System, NextSeq 500 System |
| Mechanism of action | Multiplex PCR |
| Method | Custom sequencing, Amplicon sequencing, Targeted RNA sequencing |
| Multiplexing | Up to 384-plex |
| Nucleic acid type | RNA |
| Number of amplicons | Varies |
| Specialized sample types | Blood, FFPE tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Transcript variants |
The AmpliSeq for Illumina library preparation workflow requires a panel, library prep reagents (Library Plus), and indexes. Accessory products are also available for cDNA synthesis and sample identification.
AmpliSeq cDNA Synthesis for Illumina is required to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels.
AmpliSeq Library Equalizer for Illumina is an optional but recommended easy-to-use solution for normalizing libraries while using AmpliSeq for Illumina library prep methods.
AmpliSeq ERCC RNA Spike-In Mix for Illumina is a set of external RNA controls developed by the External RNA Controls Consortium (ERCC). The controls consist of a set of unlabeled, polyadenylated transcripts that can be used with AmpliSeq for Illumina assays with transcriptome panels to quantitate differential gene expression. Requires AmpliSeq ERCC RNA Companion Panel for Illumina (sold separately by Illumina).
AmpliSeq ERCC RNA Companion Panel for Illumina is intended for use with the AmpliSeq ERCC RNA Spike-In Mix for Illumina (sold separately by Illumina) to quantitate differential gene expression when performing AmpliSeq for Illumina assays with transcriptome panels.
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
Ultra-deep sequencing of PCR amplicons enables analysis of specific genomic regions of interest. Learn more about amplicon sequencing and find comprehensive solutions.
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
High Concordance between Replicates
Libraries were prepared from 10 ng of Universal Human RNA using an AmpliSeq for Illumina Custom RNA Panel (208 amplicon panel size) and sequenced on the MiSeq System. Results show a high concordance (R2 = 0.997) between the two replicates of the same RNA sample. R2 is a statistical measurement of data correlation.
High Read Alignment
The AmpliSeq for Illumina Custom RNA Panel enables researches to analyze differential gene expression across genes of interest. To demonstrate assay performance, Human Brain Reference RNA and Universal Human RNA were analyzed using 5 AmpliSeq for Illumina Custom RNA panels of varying size (number of amplicons). Libraries were sequenced on a MiSeq System.
AmpliSeq™ for Illumina® Custom RNA Panel
20020496
Targeted custom RNA panel. Library PLUS and index adapters sold separately.
AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set B for Illumina®
20019106
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set C for Illumina®
20019107
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.
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AmpliSeq™ for Illumina® RNA Library Preparation Kit Training
20023393
1.5 day on-site training for the complete AmpliSeq™ for Illumina RNA library preparation workflow. This training can be delivered with any of the available AmpliSeq™ for Illumina RNA panels. Includes quantification of RNA input as well as best practices, troubleshooting tips, and training on Illumina-supported analysis tools specific to the RNA application workflow.
Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).
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AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.
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