AmpliSeq for Illumina Custom RNA Panel

Targeted, custom RNA research panels optimized for sequencing up to 1200 genomic targets of interest.Read More...
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What products do I need?

AmpliSeq™ for Illumina® Custom RNA Panel

20020496

Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

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Accessory Products
What accessories do I need?

Ampliseq™ cDNA Synthesis for Illumina®

20022654

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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AmpliSeq™ for Illumina® ERCC RNA Spike-In Mix

20030697

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AmpliSeq™ for Illumina® ERCC Companion Panel

20030696

Price
 
 

Product Highlights

Use AmpliSeq for Illumina Custom RNA Panels to measure gene expression in 12 to 1200 gene targets in a single assay. You can easily design custom targeted panels from a menu of over 20,000 human RefSeq genes using DesignStudio Software, a web-based assay design software tool. Key features include:

  • Fast Gene Expression Profiling
    Prepare libraries in 6 hours with < 1.5 hours hands-on time; results available in < 2 days.
  • Low-Quality, Low-Quantity Sample Input
    Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.
  • DNA-to-Analysis Solution
    Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.

An AmpliSeq for Illumina Custom RNA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.

Learn More About Ampliseq for Illumina

Frequently Purchased Together

Specifications

Product Comparison

AmpliSeq for Illumina Custom RNA Panel TruSeq Targeted RNA Expression Library Prep Kits
Assay Time 5.5-7.5 hours 1 day
Hands-On Time <1.5 hours 4 hours
Input Quantity 1 ng 50 ng RNA, 50 ng high-quality total RNA, ≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Mechanism of Action Multiplex PCR Amplification
Method Amplicon Sequencing , Custom Sequencing, Targeted RNA Sequencing Targeted RNA Sequencing
Multiplexing Up to 384-plex Up to 384 samples per sequencing run
Nucleic Acid Type RNA RNA
Specialized Sample Types Blood, FFPE Tissue FFPE Tissue, Low-Input Samples
Species Category Human Human, Mouse, Rat
System Compatibility iSeq 100 , MiniSeq , MiSeq , NextSeq 500 , NextSeq 550 MiniSeq , MiSeq , NextSeq 500 , NextSeq 550
Variant Class Transcript Variants Gene Fusions, Single Nucleotide Polymorphisms (SNPs), Transcript Variants

Method-Specific Workflow Example

 

Supporting Data and Figures

 

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