Targeted panel for investigating 203 genes associated with cancer in children and young adults.
Assay time
Hands-on time
Input quantity
The AmpliSeq Childhood Cancer Panel for Illumina provides a targeted resequencing solution for comprehensive evaluation of somatic variants associated with childhood and young adult cancers.
The AmpliSeq Childhood Cancer Panel for Illumina is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis
This ready-to-use panel detects variants within multiple pediatric cancer types, including leukemias, brain tumors, and sarcomas while saving time and efforts associated with identifying targets, designing primers, and optimizing panels.
| Assay time | 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) |
|---|---|
| Automation capability | Liquid handling robot(s) |
| Hands-on time | < 1.5 hr |
| Input quantity | 10 ng high-quality DNA or RNA |
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System |
| Method | Amplicon sequencing |
| Nucleic acid type | DNA, RNA |
| Number of reactions | 24 reactions |
| Specialized sample types | Blood, Low-input samples, Bone marrow, FFPE tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs) |
AmpliSeq cDNA Synthesis for Illumina is required to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels.
AmpliSeq Library Equalizer for Illumina is an easy-to-use solution for normalizing libraries while using AmpliSeq for Illumina library prep methods.
AmpliSeq for Illumina Sample ID Panel is a human SNP genotyping panel used to generate unique IDs for each research sample. This panel includes eight primer pairs that target validated SNPs, plus one gender determining pair and an easy workflow requiring only one additional pipetting step.
AmpliSeq for Illumina Direct FFPE DNA allows for DNA preparation and library construction from FFPE tissues using the AmpliSeq for Illumina protocol without the need for deparaffinization or DNA purification.
AmpliSeq™ for Illumina® Childhood Cancer Panel
20028446
Targeted panel for assessing somatic variants associated with childhood and young adult cancers. Sufficient for 24 samples. Library Prep reagents, index adapters and accessory products are sold separately.
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AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set B for Illumina®
20019106
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set C for Illumina®
20019107
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.
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Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).
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AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.
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AmpliSeq™ for Illumina® Sample ID Panel
20019162
Includes eight single nucleotide polymorphism (SNP)-targeting primer pairs and one gender discriminating primer pair sufficient for 96 reactions when paired with AmpliSeq Library PLUS for Illumina. Enables quick and accurate sample identification. Purchase library prep, probe panels, and index adapters separately.
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AmpliSeq™ for Illumina® Direct FFPE DNA
20023378
Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification.
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