AmpliSeq for Illumina Myeloid Panel

Targeted panel to investigate 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers.Read More...
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Panel

AmpliSeq™ for Illumina® Myeloid Panel

20024478

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Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

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Accessory Products

Ampliseq™ cDNA Synthesis for Illumina®

20022654

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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Product Highlights

The Myeloid Panel enables concurrent analysis of both DNA and RNA from blood and bone marrow samples in a single assay to study biomarkers associated with hematologic malignancies. The panel covers relevant targets for these major myeloid disorders: acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML). Key features include:

High-Value Content
  • Targets biomarkers across 69 genes including challenging targets such as CEBPA and FLT3-ITDs
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 10 ng high-quality DNA and RNA
Accurate Data
  • Detect somatic mutations down to 5% frequency using local or cloud-based analysis

The Myeloid Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS), NGS technology, and automated analysis.

The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiniSeq System Samples per run (mid output): 4 DNA, 32 RNA, 3 combined samples (based on >95% at 500x minimum coverage)
Samples per run (high output): 12 DNA, 96 RNA, 11 combined samples (based on >95% at 500x minimum coverage)
2 x 151 bp
2 x 151 bp
MiSeq System Samples per run with v2 reagents: 7 DNA, 60 RNA, 6 combined samples
Samples per run with v3 reagents: 12 DNA, 96 RNA, 11 combined samples
2 x 151 bp
2 x 151 bp

Product Comparison

AmpliSeq for Illumina Myeloid Panel AmpliSeq for Illumina Cancer Hotspot Panel v2 TruSight Myeloid Sequencing Panel
Assay Time 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 8 hours
Cancer Type Hematologic Pan-Cancer, Solid Tumor Hematologic
Content Specifications Targets biomarkers across 69 genes (40 key DNA target genes, 29 RNA fusion driver genes, and 5 gene expression levels) Hotspot regions of 50 genes with known associations to cancer Targets 54 genes associated with myeloid malignancies. Probes amplify 568 amplicons from tumor suppressor genes and mutational hotspots.
Description Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. Somatic analysis research into hotspot regions of 50 cancer-related genes. For somatic mutation detection studies in myeloid malignancies.
Hands-On Time < 1.5 hours < 1.5 hours 3 hours
Input Quantity 20 ng high-quality DNA; 10 ng high-quality RNA
(10 ng recommended per pool)
1–100 ng (10 ng recommended per pool) 50 ng
Method Amplicon Sequencing , Targeted DNA Sequencing , Targeted RNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing
Multiplexing Up to 96-plex 96 dual index combinations Up to 96-plex
Nucleic Acid Type DNA, RNA DNA DNA
Specialized Sample Types Blood, Bone Marrow Blood, FFPE Tissue Not FFPE-Compatible
Species Category Human Human Human

Method-Specific Workflow Example

 

Supporting Data and Figures

 

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