AmpliSeq for Illumina Myeloid Panel

Targeted panel to investigate 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers.

5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)

Assay time

< 1.5 hr

Hands-on time

20 ng high-quality DNA; 10 ng high-quality RNA(10 ng recommended per pool)

Input quantity

See full details in the specifications table

Overview

The Myeloid Panel enables concurrent analysis of both DNA and RNA from blood and bone marrow samples in a single assay to study biomarkers associated with hematologic malignancies. The panel covers relevant targets for these major myeloid disorders: acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML). Key features include:

Key features

  • High-value content - Targets biomarkers across 69 genes including challenging targets such as CEBPA and FLT3-ITDs

  • Fast, streamlined workflow - Prepare sequencing-ready libraries in a single day from as little as 10 ng high-quality DNA and RNA

  • Accurate data - Detect somatic mutations down to 5% frequency using local or cloud-based analysis

The Myeloid Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS), NGS technology, and automated analysis.

The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.


Specifications


Required Products

The AmpliSeq for Illumina Myeloid Panel workflow requires the Myeloid Panel oligos, one of the AmpliSeq Library PLUS kits, and at least one AmpliSeq index kit. cDNA synthesis is required for converting total RNA to cDNA.

/ Results

Applications

Example workflow


Project recommendations

Instrument Recommended number of samples Read length
MiniSeq System

Samples per run (mid output): 4 DNA, 32 RNA, 3 combined samples (based on >95% at 500x minimum coverage)
Samples per run (high output): 12 DNA, 96 RNA, 11 combined samples (based on >95% at 500x minimum coverage)

2 x 151 bp
2 x 151 bp

MiSeq System

Samples per run with v2 reagents: 7 DNA, 60 RNA, 6 combined samples
Samples per run with v3 reagents: 12 DNA, 96 RNA, 11 combined samples

2 x 151 bp
2 x 151 bp


Related applications and methods

Compare

AmpliSeq for Illumina Myeloid Panel AmpliSeq for Illumina Cancer Hotspot Panel v2 TruSight Myeloid Sequencing Panel
Assay time 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 8 hours
Cancer type Hematologic Pan-Cancer, Solid Tumor Hematologic
Content specifications Targets biomarkers across 69 genes (40 key DNA target genes, 29 RNA fusion driver genes, and 5 gene expression levels) Hotspot regions of 50 genes with known associations to cancer Targets 54 genes associated with myeloid malignancies. Probes amplify 568 amplicons from tumor suppressor genes and mutational hotspots.
Description Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. Somatic analysis research into hotspot regions of 50 cancer-related genes. For somatic mutation detection studies in myeloid malignancies.
Hands-on time < 1.5 hr < 1.5 hr 3 hr
Input quantity 20 ng high-quality DNA; 10 ng high-quality RNA
(10 ng recommended per pool)
1–100 ng (10 ng recommended per pool) 50 ng
Instruments MiSeq System, MiniSeq System MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System MiSeq System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System
Method Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing Targeted DNA sequencing, Amplicon sequencing Targeted DNA sequencing, Amplicon sequencing
Multiplexing Up to 96-plex 96 dual index combinations Up to 96-plex
Nucleic acid type DNA, RNA DNA DNA
Specialized sample types Blood, Not FFPE-Compatible, Bone Marrow FFPE Tissue Not FFPE-Compatible, Not FFPE-Compatible
Species category Human Human Human
Technology Sequencing Sequencing Sequencing
Variant class Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels) Single nucleotide polymorphisms (SNPs), Somatic variants, Insertions-deletions (indels) Somatic variants, Insertions-deletions (indels)

Selection tools:

Figures

High coverage of CEBPA gene

DNA evaluated with AmpliSeq for Illumina Myeloid Panel shows high read depth coverage across the entire transcript of the gene.

 

Panel (1)

AmpliSeq™ for Illumina® Myeloid Panel

20024478

Fixed panel includes 74 genes associated with hematologic malignancies. Kit contains 24 reactions (24 DNA libraries and 24 RNA libraries). Requires 48 reactions of LP PLUS. Library PLUS, indexes and cDNA synthesis sold separately.

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Library prep (3)

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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Index adapters (6)

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.

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Accessory products (2)

Ampliseq™ cDNA Synthesis for Illumina®

20022654

Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).

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AmpliSeq™ Library Equalizer for Illumina®

20019171

Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.

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