Respiratory Virus Oligo Panel

Highly sensitive detection and characterization of common respiratory viruses, including COVID-19 strains, with comprehensive, rapid target enrichment sequencing. Read More...
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Panel

Respiratory Virus Oligos Panel V2

20044311

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Library Prep

Illumina® RNA Prep with Enrichment, (L) Tagmentation (16 Samples)

20040536

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Illumina® RNA Prep with Enrichment, (L) Tagmentation (96 Samples)

20040537

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Index Adapters

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

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IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

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Product Highlights

Using the Respiratory Virus Oligo Panel with Illumina RNA Prep with Enrichment allows researchers to obtain next-generation sequencing (NGS) data that can confirm the presence of SARS-CoV-2 and advance variant analysis studies. The agnostic design allows for widespread identification of pathogenic respiratory viruses.

Target Enrichment for Respiratory Virus Detection

Target enrichment through hybrid–capture methods allows for highly sensitive detection, without requiring the high read depth needed for shotgun metagenomic sequencing. Additionally, this method allows for near-complete sequence data of targets and opens up applications such as variant analysis for viral evolution or viral surveillance.1

Compared to other targeted resequencing methods, such as amplicon sequencing, enrichment through hybrid capture allows for dramatically larger probe panels with more comprehensive profiling of the target regions. Additionally, the oligo probes used for hybrid–capture protocols remain effective even within highly mutagenic regions, allowing targeting of rapidly evolving viruses, such as RNA viruses.

Streamlined Workflow

This comprehensive workflow integrates sample preparation, library preparation, target enrichment, sequencing, and data analysis. The workflow is intended to enrich viral targets from total nucleic acid extraction.

Reverse transcription of extracted RNA and library preparation is performed using Illumina RNA Prep with Enrichment, followed by enrichment with the Illumina Respiratory Virus Oligo Panel. After sequencing on an Illumina NGS system, data analysis proceeds using the DRAGEN Pathogen Detection pipeline and IDbyDNA Explify Platform.

  1. Gaudin M and Desnues C. Hybrid Capture-Based Next Generation Sequencing and Its Application to Human Infectious Diseases. Front Microbiol. 2018; 9:2924.

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Specifications

Product Comparison

Respiratory Virus Oligo Panel Respiratory Pathogen ID/AMR Enrichment Kit (Powered by IDbyDNA Explify)
Assay Time < 9 hours library prep time < 9 hours library prep time
Automation Capability Liquid Handling Robots Liquid Handling Robots
Content Specifications Targets and characterizes ~40 common respiratory viruses, including SARS-CoV-2.
Description A streamlined workflow for detecting and analyzing SARS-CoV-2 and other common respiratory viruses using RNA Prep for Enrichment combined with Respiratory Virus Oligo Panel for library prep, proven Illumina sequencing, and simplified data analysis using the DRAGEN RNA Pathogen Detection App or IDbyDNA Explify RVOP app on BaseSpace Sequencing Hub. A streamlined workflow using Respiratory Pathogen ID/AMR Panel (RPIP) with Illumina RNA Prep with Enrichment, sequencing on Illumina desktop systems, and powerful analysis using IDbyDNA’s Explify Platform allows researchers to identify respiratory infections and co-infections, identify antimicrobial resistance, and perform strain typing of critical pathogens (SARS-CoV-2 and Flu A/B viruses) to study viral evolution and transmission.
Hands-on time < 2 hours library prep time < 2 hours library prep time
Input Quantity 10-100 ng 10-100 ng
Multiplexing Up to 384 samples in a single run with unique dual indexes Up to 384 samples in a single run with unique dual indexes
Species Category Human, Virus Bacteria, Fungal, Virus
Species Details Detects common respiratory viruses, including recent flu strains and SARS-CoV-2. Includes human probes as a quality feature, human probes are included in every sample. Detects respiratory pathogens (180+ bacteria, 50+ fungi, and 40+ viruses, including SARS-CoV-2) and antimicrobial resistance alleles (1200+).
System Compatibility MiniSeq , MiSeq , NextSeq 550 MiniSeq , MiSeq , NextSeq 550

Example Workflow

 

Supporting Data and Figures

 

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