Illumina Respiratory Virus Enrichment Kit

A streamlined workflow offering highly sensitive detection and characterization of common respiratory viruses, including SARS-CoV-2 strains.

< 9 hr library prep time

Assay time

< 2 hr library prep time

Hands-on time

10-100 ng

Input quantity

See full details in the specifications table

The Respiratory Virus Oligo Panel v2 (RVOP v2, Catalog no. 20044311) is now sold in comprehensive kits (Sets A-D) that include the Respiratory Virus Oligo Panel V2, library prep and enrichment reagents, and index adapters. The standalone RVOP v2 product or individual components can be purchased through  MyIllumina.

Overview

The Illumina Respiratory Virus Enrichment Kit, formerly known as the Respiratory Virus Oligo Panel (RVOP), provides an effective, streamlined method for detecting common respiratory viruses, such as SARS-CoV-2 and influenza A/B. 

  • Understand variant analysis for viral evolution or viral surveillance1

  • Determine viral transmission patterns and predict resurgence

  • Identify and characterize co-infections and their role in complex diseases

Target enrichment for respiratory virus detection

Target enrichment through the hybrid–capture method allows for highly sensitive detection without requiring the high read depth needed for shotgun metagenomic sequencing. Additionally, this method allows for dramatically larger probe panels for comprehensive profiling compared to amplicon sequencing while producing whole-genome sequencing data for the targeted viruses.

Streamlined solution for viral applications

This comprehensive workflow integrates library preparation, target enrichment, sequencing, and data analysis, giving researchers and public health scientists the ability to monitor multiple viruses and variants with one workflow


Specifications


Required products

Illumina Respiratory Virus Enrichment Kits contain the Respiratory Virus Oligo Panel (RVOP) v2, Illumina RNA Prep with Enrichment, and one of four available unique dual indexing plates. To run 384 samples at once, order all four sets (A, B, C, and D). Additional Illumina reagents required include an Illumina sequencer and the appropriate sequencing reagents and flow cell(s).
/ Results

Applications

Illumina Respiratory Virus Enrichment Kit, Illumina sequencing, and DRAGEN secondary analysis offer a streamlined workflow that enables the detection and characterization of over 40 common human respiratory viruses. 

Example workflow


Project recommendations

Instrument Recommended number of samples Read length
MiniSeq System Rapid: 20 samples, 1M clusters and a minimum of 1M single-end reads per sample 1 × 100 bp
MiSeq System v3 reagents: ~20 samples, 1M clusters and a minimum of 1 M single-end reads per sample 1 × 150 bp
NextSeq 550 System High output: 384 samples, 1M clusters and a minimum of 1M single-end reads per sample 1 × 150 bp

Related applications and methods

Compare

Illumina Respiratory Virus Enrichment Kit Respiratory Pathogen ID/AMR Enrichment Panel Kit
Assay time < 9 hr library prep time < 9 hr library prep time
Automation capability Liquid handling robots Liquid handling robots
Automation details Explore available automation methods Explore available automation methods
Content specifications Targets and characterizes ~40 common respiratory viruses, including SARS-CoV-2, influenza A and B viruses, adenovirus, rhinovirus, respiratory syncytial virus, and other common respiratory viruses. Detects respiratory pathogen DNA and RNA simultaneously and profiles antimicrobial resistance (AMR) gene expression concurrently.
Description A streamlined workflow for detecting and analyzing SARS-CoV-2 and other common respiratory viruses. The agnostic design allows for widespread identification of pathogenic viruses across multiple sample types of interest. Identify respiratory infections and co-infections, detect antimicrobial resistance markers, and perform strain typing of critical pathogens (SARS-CoV-2 and Flu A/B viruses) to study viral evolution and transmission.
Hands-on time < 2 hr library prep time < 2 hr library prep time
Input quantity 10-100 ng Based on volume (not concentration dependent)
Instruments MiSeq System, NextSeq 550 System, NextSeq 2000 System, MiniSeq System MiSeq System, NextSeq 550 System, MiniSeq System
Mechanism of action On-bead tagmentation followed by a single hybridization step On-bead tagmentation followed by a single hybridization step
Method Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment
Multiplexing Up to 384 samples in a single run with unique dual indexes Up to 384 samples in a single run with unique dual indexes
Nucleic acid type DNA, RNA DNA, RNA
Sample type details

Nasal swabs and washes, wastewater, and more

Species category Human, Virus Fungal, Virus, Bacteria
Species details Detects common respiratory viruses, including recent influenza virus strains and SARS-CoV-2. The panel includes probes to human targets as a quality feature, and therefore detects those human targets in every sample assayed. Detects respiratory pathogens (180+ bacteria, 50+ fungi, and 40+ viruses, including SARS-CoV-2) and antimicrobial resistance alleles (1200+).
Technology Sequencing Sequencing
Variant class Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs), Insertions-deletions (indels)

Selection tools

Figures

Enrichment workflow for respiratory virus detection

The streamlined NGS workflow for respiratory virus detection integrates sample preparation, library preparation, target enrichment, sequencing, and data analysis.

Highly sensitive SARS-CoV-2 detection

In a titration experiment of human saliva RNA spiked with a defined amount of virus copies, the Illumina Respiratory Virus Enrichment Kit resulted in detection of viral reads from as low as two copies of SARS-CoV-2 viral RNA.

Viruses detected by Illumina Respiratory Virus Enrichment Kit

The Respiratory Virus Oligo Panel includes 7800 probes to detect common respiratory viruses, recent flu strains, SARS-CoV-2, and human probes to act as positive controls. All probes are 80-mer oligos, spaced very close together, providing full genome coverage of all viruses in the panel.

Human coronavirus 229E Human parainfluenza virus 1 Human bocavirus 4 NI strain HBoV4-NI-385 Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Human coronavirus NL63 Human parainfluenza virus 2 KI polyomavirus Stockholm 60 Influenza A virus (A/Hong Kong/1073/99(H9N2))
Human coronavirus OC43 Human parainfluenza virus 3 WU Polyomavirus Influenza A virus (A/Texas/50/2012(H3N2))
Human coronavirus HKU1 Human parainfluenza virus 4a Human parechovirus type 1 PicoBank/HPeV1/a Influenza A virus (A/Michigan/45/2015(H1N1))
SARS-CoV-2 Human metapneumovirus (CAN97-83) Human parechovirus 6 Influenza B virus (B/Lee/1940)
Human adenovirus B1 Respiratory syncytial virus (type A) Human rhinovirus A89 Influenza B virus (B/Wisconsin/01/2010)
Human adenovirus C2 Human Respiratory syncytial virus 9320 (type B) Human rhinovirus C (strain 024) Influenza B virus (B/Brisbane/60/2008)
Human adenovirus E4 Influenza A virus (A/Puerto Rico/8/1934(H1N1)) Human rhinovirus B14 Influenza B virus (B/Colorado/06/2017)
Human bocavirus 1 (Primate bocaparvovirus 1 isolate st2) Influenza A virus (A/Korea/426/1968(H2N2)) Human enterovirus C104 strain: AK11 Influenza B virus (B/Washington/02/2019)
Human bocavirus 2c PK isolate PK-5510 Influenza A virus (A/New York/392/2004(H3N2)) Human enterovirus C109 isolate NICA08-4327 Human control genes
Human bocavirus 3 Influenza A virus (A/goose/Guangdong/1/1996(H5N1))    

Illlumina Respiratory Virus Enrichment Kit Set A (96 indexes, 96 samples)

20100469

Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set A. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina Respiratory Virus Enrichment Kit Set B (96 indexes, 96 samples)

20100470

Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set B. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina Respiratory Virus Enrichment Kit Set C (96 indexes, 96 samples)

20100471

Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set C. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina Respiratory Virus Enrichment Kit Set D (96 indexes, 96 samples)

20100472

Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set D. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Showing of

Selection summary

Product

Qty

Unit Price

FAQs

The Respiratory Virus Enrichment Kit supports a broad range of sample sources, including formalin-fixed, nasal swabs, nasal washes, and wastewater samples.

The Respiratory Virus Enrichment Kit library prep and enrichment protocol takes < 9 hr with < 2 hr of hands-on time.

When used with the MiniSeq Rapid Reagent Kit, MiniSeq Sequencing System, and DRAGEN RNA Pathogen Detection app, the entire process can be completed in < 24 hr. Learn more about the fast detection of respiratory viruses.

Yes, because this kit uses hybridization-based enrichment and contains probes for over 40 different viruses, multiple viruses can be detected. Learn more about the detection and characterization of respiratory viruses using the Illumina RNA Prep with Enrichment.

The Illumina Respiratory Virus Enrichment Kit includes free usage of the DRAGEN RNA Pathogen Detection app and the DRAGEN Targeted Microbial app, both of which analyze pathogen data and generate consensus sequences.

/ Results

Order the NovaSeq X Series

Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

Speak with a specialist

Reach out for information about our products and services, or get answers to questions about our technology.