ScriptSeq Complete Kit (H/M/R) 24 reactions
ScriptSeq Complete (H/M/R) improves sequencing efficiency, as seen in the higher percentage of usable reads that map to the genome. Universal Human Reference RNA (UHRR), Brain Reference RNA (BrRR), or FFPE breast tumor RNA samples were converted into sequencing libraries using ScriptSeq Complete or Poly(A) selection followed by ScriptSeq v2.
Cluster-ready ScriptSeq RNA-Seq libraries, virtually rRNA-free, are made in 1 day from as little as 100 ng of intact or partially degraded total RNA.
|Kit and Input Type||Total RNA Input||Removes mitochondrial rRNA||Removes cytoplasmic rRNA|
|ScriptSeq Complete Gold with high RNA input||1-5 µg||✓||✓|
|ScriptSeq Complete with high RNA input||1-5 µg||✓|
The ScriptSeq Complete Gold (H/M/R) formulation depletes mitochondrial rRNA and cytoplasmic rRNA, providing deeper sequencing coverage.
The low input versions of this kit (catalog numbers SCL6G, SCL24G, SCL6H, and SCL24H) have been discontinued. The standard kit versions can process low input RNA with a minor protocol modification.