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Sequencing, RUO, DNA

TruSight FFPE QC Kit

The Illumina TruSight FFPE QC kit uses a real-time PCR assay, targeting a 204 bp amplicon, to evaluate the quality of prospective DNA samples.

Overview

DNA extracted from formalin-fixed paraffin-embedded (FFPE) samples is degraded by the fixation process and can vary greatly in quality and usability for sequencing applications. The Illumina TruSight FFPE QC kit uses a real-time PCR assay targeting a 204 bp amplicon to evaluate the quality and suitability of prospective FFPE DNA samples for sequencing.

Key features

  • Evaluate sample quality accurately with standard real-time PCR methods

  • Perform up to 384 reactions per kit

  • Achieve clear results with a simple workflow

The Illumina TruSight FFPE QC Kit uses a real-time PCR assay to evaluate DNA sample quality. The assay is compatible with FFPE DNA extracted using commercially available kits from various suppliers for exceptional flexibility.

Each kit provides primers and reference DNA template for 384 reactions. The workflow will be familiar to labs with experience using common PCR methods, including standard real-time PCR instrumentation and reagents.

The workflow follows an easy real-time PCR protocol that can be performed with qPCR instruments and reagents from a range of manufacturers.

Specifications

Instruments MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq X Plus System, MiSeq i100 System, MiSeq i100 Plus System
Method Targeted DNA sequencing, Exome sequencing
Nucleic acid type DNA
Specialized sample types FFPE tissue
Species category Human
Technology Sequencing, Real-time PCR

Required Products

For the assay, you will need to separately purchase a qPCR master mix containing green fluorescent dye (Thermo Fisher Scientific, catalog no. A46012, or equivalent). Refer to your qPCR instrument manufacturer for specific recommendations.

/ Results

Applications

TruSight FFPE QC Kit uses a real-time PCR assay to evaluate the quality of prospective DNA samples, ensuring their suitability for downstream sequencing applications.

Related applications and methods

Cellular & Molecular Biology Research

Array and NGS technologies from Illumina enable analysis across the genome, transcriptome, and epigenome, broadening cellular research possibilities.

Resources

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Sequencing FFPE Samples: Introduction

This webinar introduces FFPE sample types, extraction methods, and library prep options, offering guidance for both new and experienced users working with degraded or limited DNA.

TruSight FFPE QC Kit

20139070

TruSight™ FFPE QC Kit uses a real-time PCR assay to evaluate the quality of prospective DNA samples.

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