AmpliSeq for Illumina Comprehensive Panel v3

Targeted DNA and RNA research panel investigating variants across 161 genes associated with a range of cancer types.Read More...
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Panel

AmpliSeq™ Comprehensive Panel v3 for Illumina®

20019109

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Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

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Ampliseq™ CD Indexes Large Volume for Illumina® (96 Indexes, 96 Samples)

20019108

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Accessory Products

Ampliseq™ cDNA Synthesis for Illumina®

20022654

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AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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Product Highlights

The AmpliSeq for Illumina Comprehensive Panel v3 offers coverage of key cancer genes, including kinase domains and genes involved in DNA repair.

Relevant Gene Content
  • Target 161 unique cancer-associated genes
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate Data
  • Detect somatic mutations down to 5% frequency using local or cloud-based analysis

The Comprehensive Panel v3 is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

The panel content spans hotspot regions, full-length genes, copy number genes, and inter- and intragenic gene fusions. This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiniSeq System 3 samples per run (high output; assumes minimum coverage of 500×) 2 × 150 bp
MiSeq System 3 samples per run with v3 reagents (assumes minimum coverage of 500×) 2 × 150 bp
NextSeq 550 System Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×) 2 × 150 bp

Product Comparison

AmpliSeq for Illumina Comprehensive Panel v3 TruSight Tumor 170 TruSight Tumor 15 AmpliSeq for Illumina Focus Panel AmpliSeq for Illumina Cancer Hotspot Panel v2
Assay Time 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) ~2 days (Library Prep) 7 hours 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer Type Solid Tumor Solid Tumor Solid Tumor Solid Tumor Pan-Cancer, Solid Tumor
Content Specifications DNA and RNA targets for 161 oncogenes Probes enrich for full coding sequences of 170 genes. Calls single nucleotide variants, small insertions, and deletions in 151 genes, amplifications in 59 genes, and fusions plus splice variants in 55 genes. Amplifies 250 amplicons from 15 genes associated with solid tumors DNA and RNA targets for 52 oncogenes Hotspot regions of 50 genes with known associations to cancer
Description Somatic analysis research on hotspot and full-length targets of genes associated with solid tumor cancers. Perform comprehensive somatic variant detection research in solid tumors using variant calling information from both DNA and RNA. Focused panel to study relevant solid tumor somatic variants in 15 genes using a simple workflow. Somatic analysis research on 52 genes associated with solid tumor cancers. Somatic analysis research into hotspot regions of 50 cancer-related genes.
Hands-On Time < 1.5 hours ~10.5 hours 3.5 hours < 1.5 hours < 1.5 hours
Input Quantity 1–100 ng (10 ng recommended per pool) 40 ng DNA and/or RNA 20 ng 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool)
Method Amplicon Sequencing , Targeted DNA Sequencing , Targeted RNA Sequencing Target Enrichment , Target Enrichment, Targeted DNA Sequencing , Targeted RNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing , Targeted RNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing
Multiplexing 96 dual index combinations Up to 32-plex for DNA using both index sets, up to 16-plex for RNA 1-24-plex 96 dual index combinations 96 dual index combinations
Nucleic Acid Type DNA, RNA RNA, DNA DNA DNA, RNA DNA
Specialized Sample Types Blood, FFPE Tissue FFPE Tissue, Low-Input Samples FFPE Tissue Blood, FFPE Tissue Blood, FFPE Tissue
Species Category Human Human Human Human Human

Method-Specific Workflow Example

 

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