Flexibility for multiple sequencing applications

The NextSeq Series enables the widest range of applications for our benchtop sequencers

NextSeq Applications & Methods

mRNA sequencing enables researchers to take a closer look at gene expression and can be used to identify isoforms, novel transcripts, and gene fusions. The NextSeq RNA sequencing solution leverages proven Illumina technology to deliver a detailed snapshot of the coding transcriptome. Sequence up to 16 transcriptomes per NextSeq run.

See how researchers are using mRNA-Seq with the NextSeq Series.

Read eBook: The Next Generation of Gene Expression Profiling

Exome sequencing analyzes the protein-coding region of the genome, as a cost-effective alternative to whole-genome sequencing. Exome sequencing with the NextSeq Series offers a simple and reliable method to identify coding variants, successfully sequencing even the most difficult genomic regions. Sequence 3–12 exomes per NextSeq run.

See how researchers are using the NextSeq Series for exome sequencing applications.

The NextSeq Series leverages proven Illumina sequencing technology, delivering high coverage to identify variants throughout the genome. With push-button sequencing and minimal hands-on time, the NextSeq whole-genome sequencing (WGS) solution enables researchers to analyze any genome efficiently, from microbes to humans. Sequence 1 human genome or up to 384 small genomes per NextSeq run.

See how researchers are using the NextSeq Series to sequence large whole-genomes:

Whole-Transcriptome Sequencing

Analyze both coding RNA and multiple forms of noncoding RNA with total RNA sequencing, for a comprehensive view of the transcriptome.

Application Note

NextSeq Series RNA-Seq Solution

Researcher Interview

Stromal Signatures May Inform Cancer Prognosis

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Coding Transcriptome Analysis

Detect both known and novel features of the RNA exome using sequence-specific capture of RNA coding regions.

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Targeted DNA Sequencing

Targeted resequencing focuses time, expenses, and analysis on sequencing only a subset of genes or genome regions of research interest.

Researcher Interview

Unmasking the Viral Etiology of Cancer and Immune Disease

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Targeted Gene Panels

Targeted gene sequencing panels contain defined probe sets focused on specific genes of interest. Both predesigned and custom panels are available.

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Methyation Sequencing

Perform epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.

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Amplicon Sequencing

Ultra-deep sequencing of PCR amplicons enables cost-effective analysis of up to hundreds of target genomic regions in one assay.

Application Note

NextSeq Series Amplicon Sequencing Solution

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De Novo Sequencing

De novo sequencing with next-generation sequencing (NGS) enables fast, accurate characterization of species without a reference genome.

Application Note

NextSeq Series WGS Solution

Researcher Interview

Delivering Sequencing Results Quickly and at a Competitive Price

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Genotyping by Sequencing

Genotyping by sequencing provides a low-cost genetic screening method to discover novel plant and animal SNPs and perform genotyping studies.

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DNA-Protein Interaction Analysis with ChIP-Seq

Combining chromatin immunoprecipitation (ChIP) assays with sequencing, ChIP-Seq is a powerful method for genome-wide surveys of gene regulation.

Researcher Interview

Next-Generation Sequencing Proves Invaluable for Biomarker Discovery

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miRNA & Small RNA Analysis

Isolate and sequence small RNA species, such as microRNA, to study the role of noncoding RNA in gene silencing and posttranscriptional regulation.

Application Note

NextSeq Series RNA-Seq Solution

Researcher Interview

Unraveling Disease Mechanisms With the NextSeq 500 System

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Translation Analysis

Ribosome profiling enables systematic monitoring of cellular translation processes and prediction of protein abundance.

Application Note

Ribosome Profiling Enables Comprehensive Translation Analysis

Researcher Interview

Casting a Genome-Wide Net Over Malaria

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Metagenomics

Comprehensively sample genes in organisms present in a complex sample to evaluate bacterial diversity and detect unculturable microorganisms.

Application Note

Metagenomic Analysis of Environmental Water Samples With the NextSeq 500 System

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Cytogenomics

Next-generation sequencing offers base-by-base, genome-wide detection of chromosomal variation to complement array-based cytogenomic analysis.

Researcher Interview

From Arrays to NGS: How Chromosomal Genetics Evolved from Structure to Disease

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Illumina NGS Methods Guide

Illumina NGS Methods Guide

Access the information you need—from genome, transcriptome, or epigenome library preparation to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive catalog designed specifically for research applications.

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