mRNA sequencing (mRNA-Seq) enables researchers to take a closer look at gene expression and can be used to identify isoforms, novel transcripts, and gene fusions. The NextSeq RNA sequencing solution leverages proven Illumina technology to deliver a detailed snapshot of the coding transcriptome. Sequence up to 16 transcriptomes per NextSeq run.
10.5 hours/4.5 hours hands-on
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
Nextera Flex for Enrichment uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time.
15–18 hours/15 minutes hands-on
Bring the power of a high-throughput sequencing system to the benchtop. The 150 cycle high-output or mid-output kits provide 2 x 75 bp reads for total RNA-Seq.
Performs read mapping, normalized abundance estimation of reference genes and transcripts, novel transcripts assembly, variant calling, and optional fusion calling.
Quickly assess novel transcript isoforms and gene expression levels from RNA-Seq Alignment results.
Exome sequencing analyzes the protein-coding region of the genome, as a cost-effective alternative to whole-genome sequencing. Exome sequencing with the NextSeq Series offers a simple and reliable method to identify coding variants, successfully sequencing even the most difficult genomic regions. Sequence 3–12 exomes per NextSeq run.
15-18 hours/15 minutes hands-on
Bring the power of a high-throughput sequencing system to the desktop. The 300 cycle high-output or mid-output kits provide 2 x 150 bp reads for exome sequencing.
Rapid alignment and variant detection for small, structural, and copy number variant calling, variant annotation, and enrichment metrics calculation.
The NextSeq Series leverages proven Illumina sequencing technology, delivering high coverage to identify variants throughout the genome. With push-button sequencing and minimal hands-on time, the NextSeq whole-genome sequencing (WGS) solution enables researchers to analyze any genome efficiently, from microbes to humans. Sequence 1 human genome or up to 384 small genomes per NextSeq run.
6 hours/4 hours hands-on
Generate WGS libraries and efficiently interrogate samples with limited available DNA.
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
26–29 hours/15 minutes hands-on
Bring the power of a high-throughput sequencing system to the desktop. The 300 cycle high-output kit provides 2 x 150 bp reads for whole-genome sequencing.
Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications.Access Guide