Illumina Sequencing Methods

Comprehensive NGS solutions including library preparation, sequencing systems, and data analysis


See What Sequencing Can Do For You

Sequencing may be utilized to determine the order of nucleotides in small targeted genomic regions or entire genomes. Illumina sequencing enables a wide variety of applications, allowing researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism. Next-generation sequencing (NGS) methods differ primarily by how the DNA or RNA samples are prepared and the data analysis options used.

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Key Sequencing Methods

DNA Sequencing

Analyze the entire genome, focus on regions of interest with whole-exome and targeted sequencing, or study DNA-protein interactions.

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RNA Sequencing

Take advantage of a broad range of techniques, from targeted RNA to single-cell and whole-transcriptome sequencing.

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Methylation Sequencing

Both genome-wide analysis and targeted approaches can provide insight into methylation patterns at a single nucleotide level.

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Library Preparation Solutions

Library preparation options are available for a broad range of sequencing methods, including whole-genome sequencing, whole-exome and targeted sequencing, RNA sequencing, methylation sequencing, and more. These solutions accommodate a variety of throughput needs, from manual protocols to fully automated workstations.

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Methods Guide

All the information you need, from BeadChips to library preparation to sequencer selection and analysis. Select the best tools for your lab.

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Beginner's Guide to Next-Generation Sequencing

Considering bringing next-generation sequencing to your lab, but unsure where to start? These resources cover key topics in NGS and are designed to help you plan your first experiment.

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Beginner's Guide to Next-Generation Sequencing
Cancer Research
Cancer Research

NGS-based sequencing enables cancer researchers to detect rare somatic variants, tumor subclones, and circulating DNA fragments. Learn more about sequencing for cancer research.

Microbiology Research
Microbial Sequencing

From environmental metagenomics studies to infectious disease surveillance and more, NGS-based sequencing can help researchers gain genetic insight into bacteria and viruses. Learn more about microbial genomics.

Complex Disease Research
Complex Disease Research

Illumina sequencing is introducing new avenues for understanding immunological, neurological, and other complex disorders on a molecular level. Learn more about complex disease genomics.

Reproductive and Genetic Health
Reproductive and Genetic Health

Illumina sequencing and array technologies deliver fast, accurate information that can guide choices along the reproductive and genetic health journey. Find reproductive and genetic health solutions.

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NGS Introduction
Introduction to NGS

NGS has revolutionized the biological sciences. Learn how it compares to conventional methods, and find out how Illumina technology works.

Explore NGS
New Technologies for HIV Research
HIV-1 Tropism Studies

Deep sequencing enables researchers to assess HIV Type 1 coreceptor usage.

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Sequencing Troubleshooting Tips
Sequencing Troubleshooting Tips

These short videos provide expert tips for common issues including overclustering, inconsistent quantitation, and sequencing through the insert.

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