Illumina Sequencing Methods

Comprehensive NGS solutions including library preparation, sequencing systems, and data analysis

Sequencing Methods

See What Sequencing Can Do For You

Illumina sequencing enables a wide variety of applications, allowing researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism. Next-generation sequencing (NGS) methods differ primarily by how the DNA or RNA samples are prepared and the data analysis options used.

Decade of Sequencing
One Decade of Sequencing

Explore the breakthroughs, advancements, and progress.
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Key Sequencing Methods

DNA Sequencing

Analyze the entire genome, focus on regions of interest with whole-exome and targeted sequencing, or study DNA-protein interactions.

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RNA Sequencing

Take advantage of a broad range of sequencing techniques, from targeted RNA to single-cell and whole-transcriptome options.

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Methylation Sequencing

Both genome-wide analysis and targeted sequencing approaches can provide insight into methylation patterns at a single nucleotide level.

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Sequencing Library Preparation

Library preparation options are available for all sequencing methods, including whole-genome sequencing, whole-exome and targeted sequencing, RNA sequencing, methylation sequencing, and more. These solutions accommodate a range of throughput needs, from manual protocols to fully automated workstations.

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Methods Guide

All the information you need, from BeadChips to library preparation to sequencer selection and analysis. Select the best tools for your lab.

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Sequencing Method Explorer

Use this interactive tool to explore experimental next-generation sequencing (NGS) library preparation methods compiled from the scientific literature.

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Sequencing Method Explorer
Cancer Research
Cancer Research

NGS-based cancer sequencing methods enable researchers to detect rare somatic variants, tumor subclones, and circulating DNA fragments. Learn more about cancer sequencing.

Microbiology Research
Microbial Sequencing

From environmental metagenomics studies to infectious disease surveillance and more, NGS-based sequencing can help researchers gain genetic insight into bacteria and viruses. Learn more about microbial sequencing.

Complex Disease Research
Complex Disease Research

Sequencing and array technologies are introducing new avenues for understanding immunological, neurological, and other complex disorders on a molecular level. Learn more about complex disease genomics.

Reproductive and Genetic Health
Reproductive and Genetic Health

Illumina sequencing and array technologies deliver fast, accurate information that can guide choices along the reproductive and genetic health journey. Learn more about reproductive and genetic health.

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Compare Sequencing Platforms
Find the Right Sequencing Platform

Use this interactive tool to compare sequencing systems and identify the best option for your lab and applications.

Launch Tool
NGS Introduction
Introduction to NGS

With its unprecedented throughput, scalability, and speed, NGS enables biological studies at a level never before possible.

Explore NGS
NextSeq 500
Improve Your Sequencing Results

NextSeq 500 v2 Kits offer better data quality, an improved signal to noise ratio, and a simpler workflow.

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Sequencing Troubleshooting Tips
Sequencing Troubleshooting Tips

These short videos provide expert tips for common issues including overclustering, inconsistent quantitation, and sequencing through the insert.

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