Targeted research panel investigating somatic and germline variants in BRCA1 and BRCA2.
Assay time
Hands-on time
Input quantity
The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes.
The BRCA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
BRCA1 and BRCA2 are human tumor suppressor genes that, when carrying specific mutations, have been implicated in an increased risk for breast and ovarian cancers. This ready-to-use panel saves researchers the time and effort of identifying targets, designing primers, and optimizing panels.
| Assay time | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
|---|---|
| Cancer type | Solid Tumor |
| Content specifications | Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2 |
| Description | Germline and somatic analysis studies of BRCA1 and BRCA2. |
| Hands-on time | <1.5 hr |
| Input quantity | 1–100 ng (10 ng recommended per pool) |
| Instruments | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System |
| Mechanism of action | Multiplex PCR |
| Method | Amplicon Sequencing, Targeted DNA Sequencing |
| Multiplexing | 96 dual index combinations |
| Nucleic acid type | DNA |
| Number of amplicons | 265 total. 2 pools. (Pool 1: 132 amplicons. Pool 2: 133 amplicons). |
| Specialized sample types | Blood, FFPE Tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Somatic variants, Germline variants, Insertions-deletions (indels) |
The AmpliSeq for Illumina BRCA Panel workflow requires the BRCA Panel, Library PLUS kit, and index adapter.
AmpliSeq for Illumina BRCA Panel
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| iSeq 100 System | 12 samples per run (assumes minimum coverage of 500×) |
2 × 150 bp |
| MiniSeq System | Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×) |
2 × 150 bp |
| MiSeq System | Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80 |
2 × 150 bp |
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.
| AmpliSeq for Illumina BRCA Panel | TruSight Cancer | |
|---|---|---|
| Assay time | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 1.5 days |
| Cancer type | Solid Tumor | Pan-Cancer |
| Content specifications | Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2 | Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers. |
| Description | Germline and somatic analysis studies of BRCA1 and BRCA2. | Germline mutation detection research for common and rare cancers. |
| Hands-on time | <1.5 hr | 5 hr |
| Input quantity | 1–100 ng (10 ng recommended per pool) | 50 ng DNA |
| Instruments | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System |
| Mechanism of action | Multiplex PCR | |
| Method | Amplicon Sequencing, Targeted DNA Sequencing | Target Enrichment, Targeted DNA Sequencing |
| Multiplexing | 96 dual index combinations | Up to 96-plex |
| Nucleic acid type | DNA | DNA |
| Number of amplicons | 265 total. 2 pools. (Pool 1: 132 amplicons. Pool 2: 133 amplicons). | |
| Specialized sample types | Blood, FFPE Tissue | Not FFPE-Compatible |
| Species category | Human | Human |
| Variant class | Single nucleotide polymorphisms (SNPs), Somatic variants, Germline variants, Insertions-deletions (indels) | Germline variants |
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
AmpliSeq™ BRCA Panel for Illumina®
20019168
Targeted panel that assesses all exons of BRCA1 and BRCA2 genes. Kit contains 24 reactions. Library PLUS and index sold separately.
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AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set B for Illumina®
20019106
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set C for Illumina®
20019107
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.
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AmpliSeq™ for Illumina® Direct FFPE DNA
20023378
Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification.
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AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.
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