The Illumina Ribo-Zero Plus Microbiome rRNA Depletion Kit is a streamlined RNA-to-analysis solution for metatranscriptome sequencing of complex microbial samples, including stool.
Read the data sheet to learn more about the metatranscriptomics applications.
|Ribo-Zero Plus Microbiome rRNA Depletion Kit||Ribo-Zero Plus rRNA Depletion Kit|
|Description||Depletion of abundant transcripts from host and bacterial rRNA for metatranscriptomics research.||Single-tube depletion of abundant transcripts from multiple species or transcripts|
|Input Quantity||Minimum 25 ng of total RNA, 50 ng recommended for optimal performance||1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples|
|System Compatibility Details||Library prep is designed to be compatible with all lllumina sequencing systems, and is recommended to be used with NextSeq 500, NextSeq 550, NextSeq 1000, NextSeq 2000, and NovaSeq 6000 Systems.||Library prep is designed to be compatible with all lllumina sequencing systems, and is extensively validated on the NextSeq 500, NextSeq 550 and NovaSeq 6000 Systems.|
|Method||Whole-Transcriptome Sequencing, Metatranscriptome Sequencing||Whole-Transcriptome Sequencing|
|Variant Class||Novel Transcripts, Transcript Variants||Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants|
|Species Category||Bacteria, Human, Mouse, Rat||Bacteria, Human, Mouse, Rat|
|Species Details||Depletion of abundant rRNA transcripts from complex bacterial (microbiome) samples including stool samples, in addition to all transcripts listed in Ribo-Zero Plus.||Depletion of abundant transcripts from multiple species including: human cytoplasmic & mitochondria rRNA, mouse rRNA, rat rRNA, E. coli and B. subtilis rRNA, human beta globin transcripts|
|System Compatibility||NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000||HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000|
|Specialized Sample Types||Microbiome Samples||FFPE Tissue, Low-Input Samples|
|Nucleic Acid Type||RNA||RNA|
See how Drs. Tonya Ward, Emily Hollister, and Le François of Diversigen and DNA Genotek developed workflows for metatranscriptomic studies and read their views for this research area’s potentially impactful future.See Spotlight
In this webinar, you'll hear from three leading experts in the field as they share their insights and key considerations on sample collection and processing, rRNA depletion, and bioinformatic analysis.Watch Webinar
Robust ribodepletion with the Ribo-Zero Plus Microbiome Depletion kit. The Ribo-Zero Plus Microbiome Depletion kit effectively depletes rRNA (5S, 16S, and 23S) levels for human and bacterial species in a single-tube reaction. Results are compared to Ribo-Zero Plus rRNA Depletion Kit and a commercially available ribodepletion kit from Company X. Two stool mixtures were tested. Sample 1 is a mixture containing both adult and infant human stool samples, while Sample 2 is a commercially available stool sample from Zymo (Cat no. 6331).
Application Note | PDF | 2 versions
Data Sheet | PDF | 6 versions
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