TruSight Myeloid Sequencing Panel

This sequencing research panel targets variants associated with myeloid malignancies such as AML, MDS, MPN, CML, CMML, and JMML.

8 hours

Assay time

3 hr

Hands-on time

50 ng

Input quantity

See full details in the specifications table

Overview

The TruSight Myeloid Sequencing Panel uses expert-defined content and proven next-generation sequencing (NGS) technology to identify somatic variants in myeloid malignancies.

The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in:

  • Acute myeloid leukemia (AML)

  • Myelodysplastic syndrome (MDS)

  • Myeloproliferative neoplasms (MPN)

  • Chronic myelogenous leukemia (CML)

  • Chronic myelomonocytic leukemia (CMML)

  • Juvenile myelomonocytic leukemia (JMML)

The TruSight Myeloid Sequencing Panel covers 15 full genes (exons only) and 39 additional genes where oncogenic hotspots are covered, providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test. The result is an accurate, cost-effective solution that enables researchers to profile liquid tumors. View the gene list in the "Figures" section.

TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller. Filtering and annotation can then be performed using variant analysis software.


Specifications


Required products

Each TruSight Myeloid library preparation kit (96 samples) requires the following accessory kits:

  • TruSeq Custom Amplicon Index Kit, (96 Indices, 384 Samples) FC-130-1003:  One Index kit required for every 4 TruSight Myeloid kits.
  • TruSeq Custom Amplicon Filter Plate (1 Plate), FC-130-1006:  One per TruSight Myeloid kit (96 samples).
  • TruSeq Index Plate Fixture and Collar Kit (2 Each), FC-130-1007: Facilitates pipetting of sequence adapters, indexing primers, and reagents prior to PCR amplification. One-time purchase only (reusable).
/ Results

Applications

Example workflow

1
Prep

TruSight Myeloid Sequencing Panel


Project recommendations

Instrument Recommended number of samples Read length
MiniSeq System

Samples per run: mid output: 2-3, high output: 8 (based on 95% amplicons at > 500x coverage)

2 × 150 bp (max recommended)

MiSeq System

8 samples per run with v3 reagents (based on 95% amplicons at > 500x coverage)

2 × 150 bp (max recommended)

NextSeq 550 System

Samples per run: mid output: 48, high output: 96 (based on 95% amplicons at > 500x coverage)

2 × 150 bp (max recommended)


Related applications and methods

Compare

TruSight Myeloid Sequencing Panel AmpliSeq for Illumina Myeloid Panel AmpliSeq for Illumina Cancer Hotspot Panel v2
Assay time 8 hours 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer type Hematologic Hematologic Pan-Cancer, Solid Tumor
Content specifications Targets 54 genes associated with myeloid malignancies. Probes amplify 568 amplicons from tumor suppressor genes and mutational hotspots. Targets biomarkers across 69 genes (40 key DNA target genes, 29 RNA fusion driver genes, and 5 gene expression levels) Hotspot regions of 50 genes with known associations to cancer
Description For somatic mutation detection studies in myeloid malignancies. Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. Somatic analysis research into hotspot regions of 50 cancer-related genes.
Hands-on time 3 hr < 1.5 hr < 1.5 hr
Input quantity 50 ng 20 ng high-quality DNA; 10 ng high-quality RNA
(10 ng recommended per pool)
1–100 ng (10 ng recommended per pool)
Instruments MiSeq System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System MiSeq System, MiniSeq System MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System
Method Targeted DNA sequencing, Amplicon sequencing Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing Targeted DNA sequencing, Amplicon sequencing
Multiplexing Up to 96-plex Up to 96-plex 96 dual index combinations
Nucleic acid type DNA DNA, RNA DNA
Specialized sample types Not FFPE-Compatible, Not FFPE-Compatible Blood, Not FFPE-Compatible, Bone Marrow FFPE Tissue
Species category Human Human Human
Technology Sequencing Sequencing Sequencing
Variant class Somatic variants, Insertions-deletions (indels) Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels) Single nucleotide polymorphisms (SNPs), Somatic variants, Insertions-deletions (indels)

Figures

TruSight Myeloid Sequencing Panel Gene List

ABL1 CEBPA HRAS MYD88 SF3B1
ASXL1 CSF3R IDH1 NOTCH1 SMC1A
ATRX CUX1 IDH2 NPM1 SMC3
BCOR DNMT3A IKZF1 NRAS SRSF2
BCORL1 ETV6/TEL JAK2 PDGFRA STAG2
BRAF EZH2 JAK3 PHF6 TET2
CALR FBXW7 KDM6A PTEN TP53
CBL FLT3 KIT PTPN11 U2AF1
CBLB GATA1 KRAS RAD21 WT1
CBLC GATA2 MLL RUNX1 ZRSR2
CDKN2A GNAS MPL SETBP1  

TruSight Myeloid Sequencing Panel (96 samples)

FC-130-1010

TruSeq Custom Amplicon-based library preparation reagents for 54 genes (either full genes or targeted regions) associated with myeloid malignancies. Includes library preparation reagents and TruSight Myeloid oligos sufficient for 96 samples.

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TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples)

FC-130-1003

Each TruSeq Custom Amplicon Index Kit includes 96 unique indexes for preparation of up to 384 samples.

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TruSeq Custom Amplicon Filter Plate (1 plate)

FC-130-1006

Includes a single replacement filter plate for use with the TruSeq Custom Amplicon assay.

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TruSeq Index Plate Fixture & Collar Kit (2 each)

FC-130-1007

Assists in correctly arranging index primers for PCR amplification step. Includes two (2) TruSeq Index Plate Fixtures and two (2) Plate Collars.

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