The AmpliSeq for Illumina Transcriptome Human Gene Expression Panel is a highly multiplexed targeted resequencing assay for measuring expression levels of over 20,000 human RefSeq genes in a single assay. Key features include:
Prepare libraries in 6 hours with < 1.5 hours hands-on time; results available in < 2 days.
Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.
Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.
The Transcriptome Human Gene Expression Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| AmpliSeq for Illumina Transcriptome Human Gene Expression Panel | TruSeq Stranded Total RNA | TruSeq Stranded Total RNA with Ribo-Zero Globin | |
|---|---|---|---|
| Content Specifications | > 95% of human RefSeq genes; 20,802 genes; captures human transcriptome | Captures coding RNA plus multiple forms of non-coding RNA | Captures coding RNA plus multiple forms of non-coding RNA |
| Description | Study the expression levels of >20,000 human RefSeq genes using sequence-specific amplicons. | For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. | For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA. |
| Input Quantity | 1-100 ng RNA (10 ng recommended) | 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. | 0.1 – 1 ug high-quality purified total RNA from blood |
| Method | Exome Sequencing , mRNA Sequencing , Targeted RNA Sequencing | Whole-Transcriptome Sequencing | Whole-Transcriptome Sequencing |
| Specialized Sample Types | Blood, FFPE Tissue | FFPE Tissue | Blood |
| Species Category | Human | Human, Mouse, Rat | Human, Mouse, Rat |
Sample Identification with the AmpliSeq for Illumina Sample ID Panel Application Note
Application Note | HTML
Data Sheet | HTML | 5 versions
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Gene List
product_file | EXCEL< 1 MB
Illumina Transcriptome Human Gene Expression Panel Documentation
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Reference Guide Documentation
Illumina Adapter Sequences Document Documentation
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Checklist Documentation
Index Adapters Pooling Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Support
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
Rapidly prepare amplicon libraries for Illumina sequencers using AmpliSeq for Illumina library preparation and index adapters.
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
