The AmpliSeq for Illumina Transcriptome Human Gene Expression Panel is a highly multiplexed targeted resequencing assay for measuring expression levels of over 20,000 human RefSeq genes in a single assay. Key features include:
Prepare libraries in 6 hours with < 1.5 hours hands-on time; results available in < 2 days.
Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.
Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.
The Transcriptome Human Gene Expression Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| AmpliSeq for Illumina Transcriptome Human Gene Expression Panel | Illumina Stranded Total RNA Prep with Ribo-Zero Plus | TruSeq Stranded Total RNA | TruSeq Stranded Total RNA with Ribo-Zero Globin | |
|---|---|---|---|---|
| Content Specifications | > 95% of human RefSeq genes; 20,802 genes; captures human transcriptome | Captures coding RNA plus multiple forms of non-coding RNA | Captures coding RNA plus multiple forms of non-coding RNA | Captures coding RNA plus multiple forms of non-coding RNA |
| Description | Study the expression levels of >20,000 human RefSeq genes using sequence-specific amplicons. | For whole-transcriptome sequencing studies of multiple sample types. Includes Ribo-Zero plus module for single-tube depletion of abundant transcripts from multiple species | For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. | For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA. |
| Input Quantity | 1-100 ng RNA (10 ng recommended) | 1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples | 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. | 0.1 – 1 ug high-quality purified total RNA from blood |
| Method | Exome Sequencing , mRNA Sequencing , Targeted RNA Sequencing | Whole-Transcriptome Sequencing | Whole-Transcriptome Sequencing | Whole-Transcriptome Sequencing |
| Specialized Sample Types | Blood, FFPE Tissue | FFPE Tissue, Low-Input Samples | FFPE Tissue | Blood |
| Species Category | Human | Bacteria, Human, Mouse, Rat, Virus | Human, Mouse, Rat | Human, Mouse, Rat |
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Sample Identification with the AmpliSeq for Illumina Sample ID Panel Application Note
Application Note | HTML
Data Sheet | HTML | 5 versions
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Gene List
product_file | EXCEL< 1 MB
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Checklist Documentation
Illumina Transcriptome Human Gene Expression Panel Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Reference Guide Documentation
Illumina Adapter Sequences Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Support
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MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.
Targeted research panel investigating somatic and germline variants in BRCA1 and BRCA2.
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
