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The AmpliSeq for Illumina Transcriptome Human Gene Expression Panel is a highly multiplexed targeted resequencing assay for measuring expression levels of over 20,000 human RefSeq genes in a single assay. Key features include:
Prepare libraries in 6 hours with < 1.5 hours hands-on time; results available in < 2 days.
Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.
Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.
The Transcriptome Human Gene Expression Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| AmpliSeq for Illumina Transcriptome Human Gene Expression Panel | TruSeq Stranded Total RNA | TruSeq Stranded Total RNA with Ribo-Zero Globin | |
|---|---|---|---|
| Content Specifications | > 95% of human RefSeq genes; 20,802 genes; captures human transcriptome | Captures coding RNA plus multiple forms of non-coding RNA | Captures coding RNA plus multiple forms of non-coding RNA |
| Description | Study the expression levels of >20,000 human RefSeq genes using sequence-specific amplicons. | For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. | For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA. |
| Input Quantity | 1-100 ng RNA (10 ng recommended) | 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. | 0.1 – 1 ug high-quality purified total RNA from blood |
| Method | Exome Sequencing , mRNA Sequencing , Targeted RNA Sequencing | Whole-Transcriptome Sequencing | Whole-Transcriptome Sequencing |
| Specialized Sample Types | Blood, FFPE Tissue | FFPE Tissue | Blood |
| Species Category | Human | Human, Mouse, Rat | Human, Mouse, Rat |
Sample Identification with the AmpliSeq for Illumina Sample ID Panel Application Note
Application Note | HTML
Ampliseq for Illumina Transcriptome Gene Expression Panel Data Sheet
Data Sheet | HTML
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Gene List
product_file | EXCEL < 1 MB
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
Rapidly prepare amplicon libraries for Illumina sequencers using AmpliSeq for Illumina library preparation and index adapters.
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
