AmpliSeq for Illumina Transcriptome Human Gene Expression Panel

Targeted panel that measures expression levels of >20,000 human RefSeq genes.Read More...
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Panel

AmpliSeq™ Transcriptome Human Gene Expression Panel for Illumina®

20019170

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Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ CD Indexes Set A for Illumina® (96 Indexes, 96 Samples)

20019105

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Accessory Products

Ampliseq™ cDNA Synthesis for Illumina®

20022654

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AmpliSeq™ for Illumina® Sample ID Panel

20019162

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AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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Product Highlights

The AmpliSeq for Illumina Transcriptome Human Gene Expression Panel is a highly multiplexed targeted resequencing assay for measuring expression levels of over 20,000 human RefSeq genes in a single assay. Key features include:

Fast Gene Expression Profiling

Prepare libraries in 6 hours with < 1.5 hours hands-on time; results available in < 2 days.

Low-Quality, Low-Quantity Sample Input

Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.

DNA-to-Analysis Solution

Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.

The Transcriptome Human Gene Expression Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

Specifications

Product Comparison

AmpliSeq for Illumina Transcriptome Human Gene Expression Panel TruSeq Stranded Total RNA TruSeq Stranded Total RNA with Ribo-Zero Globin
Content Specifications > 95% of human RefSeq genes; 20,802 genes; captures human transcriptome Captures coding RNA plus multiple forms of non-coding RNA Captures coding RNA plus multiple forms of non-coding RNA
Description Study the expression levels of >20,000 human RefSeq genes using sequence-specific amplicons. For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA.
Input Quantity 1-100 ng RNA (10 ng recommended) 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. 0.1 – 1 ug high-quality purified total RNA from blood
Method Exome Sequencing , mRNA Sequencing , Targeted RNA Sequencing Whole-Transcriptome Sequencing Whole-Transcriptome Sequencing
Specialized Sample Types Blood, FFPE Tissue FFPE Tissue Blood
Species Category Human Human, Mouse, Rat Human, Mouse, Rat

Method-Specific Workflow Example

 

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