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Pillar oncoReveal Nexus 21 Gene Panel

A robust next-generation sequencing (NGS) assay, based on the single-tube SLIMamp workflow, designed to interrogate genes relevant to both blood and solid organ cancers.

< 8 hrs assay time; < 24 hrs sample to result

Assay time

~3 hr

Hands-on time

20–60 ng DNA

Input quantity

See full details in the specifications table

Overview

The oncoReveal Nexus 21 Gene Panel is a robust NGS research assay designed to interrogate 21 genes relevant to both blood cancers and solid organ cancers. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp) technology, a tiled amplicon-based library prep chemistry, for efficient single-tube target enrichment.

Key features

  • Access a rapid workflow to go from sample to report in < 24 hours

  • Achieve variant detection as low as 1% VAF even with limited DNA input or poor sample quality

  • Improve lab efficiency and reduce "no calls," and repeat testing to simplify data interpretation

The panel detects single nucleotide variants (SNVs), small and medium insertion/deletions (indels), and internal tandem duplicates (ITDs) in DNA isolated from blood or FFPE tissue.

This panel uses a streamlined enrichment workflow for fast preparation of sequencing libraries, helping labs move from extracted DNA to data generation within the same day.

Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab.

Specifications

Assay time < 8 hrs assay time; < 24 hrs sample to result
Content specifications 21 genes
Description NGS assay that analyzes key mutations in 21 genes relevant to solid tumors and blood cancers.
Hands-on time ~3 hr
Input quantity 20–60 ng DNA
Instruments MiSeq System, MiSeqDx in Research Mode, MiSeq i100 System, MiSeq i100 Plus System
Mechanism of action Multiplex PCR using tiled amplicon
Method Targeted DNA sequencing, Amplicon sequencing
Nucleic acid type DNA
Number of amplicons Pool 1: 112 amplicons
Number of reactions 24 reactions per kit
On-target reads > 99%
Specialized sample types Blood, Low-input samples, FFPE tissue
Species category Human
Target insert size 162 bp
Technology Sequencing
Variant class Single nucleotide variants (SNVs), Insertions-deletions (indels), Internal tandem duplications (ITDs)

Required Products

Pillar oncoReveal Nexus 21 Gene Panel also requires the use of a Pillar index kit and sequencing reagents. If secondary analysis and additional insights are desired, DRAGEN Amplicon and Illumina Connected Insights are compatible.

/ Results

Applications

The Pillar oncoReveal Nexus 21 Gene Panel analyzes DNA extracted from solid tumors or blood cancers in under 8 hours of sample processing time with just 3 hours of hands-on time.

Example workflow

1
Prep

Pillar oncoReveal Nexus 21 Gene Panel

2
Sequence
3
Analyze

Documentation

Product literature
Support documentation

Compare

Pillar oncoReveal Nexus 21 Gene Panel Pillar oncoReveal Multi-Cancer CNV + RNA Fusion Panel Pillar oncoReveal Myeloid Panel TruSight Oncology 500 v2
Assay time < 8 hrs assay time; < 24 hrs sample to result 48 hr from sample to report 48 hr from sample to report 3–4 days from sample input to final results
Content specifications 21 genes 78-gene small pan-tumor oncology panel Targeted sequencing of DNA from 523 genes and RNA from 55 genes for a total of 1.94 Mb panel size. MSI and TMB measurement included.  The included HRD panel † includes coverage of ~25K SNPs to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST) powered by Myriad Genetics. 

†TruSight Oncology 500 v2 is not available for sale in Japan
Description NGS assay that analyzes key mutations in 21 genes relevant to solid tumors and blood cancers. 78- gene amplicon-based panel with DNA & RNA content, simple single tube workflow 58-gene amplicon based DNA panel, simple single tube workflow Enables comprehensive genomic profiling of solid tumors using DNA and RNA from FFPE tissue. Provides variant detection and biomarker assessment from a single sample for broad tumor characterization.
Hands-on time ~3 hr ~3 hr ~3 hr ~3.25 hrs for automated workflow
~5-7 hrs for manual workflow
Input quantity 20–60 ng DNA 5–80 ng DNA, 10–50 ng RNA 10–80 ng DNA (20 ng recommended) 30 ng DNA (as low as 10 ng), 40 ng RNA (as low as 20 ng)
Method Targeted DNA sequencing, Amplicon sequencing Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing Targeted DNA sequencing, Amplicon sequencing Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment
Nucleic acid type DNA DNA, RNA DNA DNA, RNA
Number of amplicons Pool 1: 112 amplicons Pool 1 (DNA): 341 amplicons. Pool 2 (RNA): >80 amplicons plus MET exon 14 skipping 1 pool, 766 amplicons
Number of reactions 24 reactions per kit 24 DNA/24 RNA 24 reactions
On-target reads > 99% 99.5% ± 0.1% 99.6% ± 0.2%
Specialized sample types Blood, Low-input samples, FFPE tissue Blood, Low-input samples, FFPE tissue Blood, Low-input samples FFPE tissue
Species category Human Human Human Human
Target insert size 162 bp Average: 120 bp (range 86-185 bp) Average: 215 bp
Technology Sequencing Sequencing Sequencing Sequencing
Variant class Single nucleotide variants (SNVs), Insertions-deletions (indels), Internal tandem duplications (ITDs) Gene fusions, Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) Gene fusions, Loss of heterozygosity (LOH), Somatic variants, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs), Tumor mutational burden (TMB), Genomic instability score (GIS), Microsatellite instability (MSI), Novel transcripts, Single nucleotide polymorphisms (SNPs), Structural variants

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Figures

Gene list

Pillar oncoReveal Nexus 21 Gene Panel includes 21 genes

Panel specifications

Pillar oncoReveal Nexus 21 Gene Panel specifications for interrogating DNA from FFPE tissue or blood

Library prep (3)

OncoReveal Nexus 21 Gene Panel

HDA-HS-1012-24

OncoReveal Nexus 21 Gene Panel

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Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

IDX-PI-1001-96

Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

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Kit D, indices PI501-8, PI701-12

IDX-PI-1004-192

Kit D, indices PI501-8, PI701-12

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