Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome

Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with exceptional study flexibility. Read More...
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Library Prep

Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (16 Samples)

20040525

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Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (96 Samples)

20040529

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Illumina® Stranded Total RNA Prep with Ligation, Ribo-Zero Plus Microbiome (96 Samples)

20072063

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Index Adapters

IDT® for Illumina® RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)

20040553

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IDT® for Illumina® RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)

20040554

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IDT® for Illumina® RNA UD Indexes Set C, Ligation (96 Indexes, 96 Samples)

20040555

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IDT® for Illumina® RNA UD Indexes Set D, Ligation (96 Indexes, 96 Samples)

20040556

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Services

Illumina Stranded Total RNA Library Prep Training

20044764

Product Highlights

Illumina Total RNA Prep with Ribo-Zero Plus provides exceptional performance for the analysis of coding and multiple forms of noncoding RNA. Gain high-confidence discovery of alternative transcripts, gene fusions, and allele-specific expression.

Illumina Stranded Total RNA Prep with Ribo-Zero Plus Microbiome effectively depletes unwanted host and bacterial rRNA from thousands of bacterial strains commonly found in the gut for high-quality metatranscriptomics applications.

Read the data sheet to learn more about metatranscriptomics applications.

Exceptional Performance

Illumina Total RNA Prep with Ribo-Zero Plus is based on TruSeq ligation technology, which has been cited in over 9,926 publications since 2011. This ligation method results in high coverage uniformity, precise strand information, and robust and reliable library preparation even from degraded samples.

Learn more about ligation technology

Flexibility for Many Study Designs

Illumina Total RNA Prep with Ribo-Zero Plus supports a broad range of RNA inputs, from 1 ng to 1000 ng1. It’s compatible with various sample types, including formalin-fixed paraffin-embedded (FFPE) and other low-quality samples. The included Ribo-Zero Plus removes abundant RNA from multiple species, including human, mouse, rat, bacteria, and epidemiology samples.

Find compatible species

Illumina Stranded Total RNA Prep with Ribo-Zero Plus Microbiome provides robust depletion of abundant rRNA in complex microbial samples, including stool samples, for metatranscriptomic studies. This kit accommodates a broad range of RNA inputs from 25 ng to 1000 ng2, and depletes rRNA from human, mouse, rat, and thousands of bacterial species.

Fast, Automation-friendly Workflow

Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.

Explore library prep automation solutions

Sequencing Scalability

Multiplex up to 384 samples and sequence in a single run. The number of samples sequenced per run depends on the throughput capacity of the sequencer.

1This input range is applicable to high-quality RNA. We recommend 10 ng input for best results and for FFPE or degraded samples.

2Minimum recommended input for Ribo-Zero Plus Microbiome is 25 ng.

Frequently Purchased Together

Specifications

Product Comparison

Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome TruSeq Stranded Total RNA TruSeq Stranded Total RNA with Ribo-Zero Globin
Assay Time ~7 hours 11.5 hours
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA Captures coding RNA plus multiple forms of non-coding RNA
Description For whole-transcriptome sequencing studies of multiple sample types. Includes Ribo-Zero plus module for single-tube depletion of abundant transcripts from multiple species For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA.
Hands-On Time < 3 hours 5.5 hours
Input Quantity 1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples 0.1 – 1 ug high-quality purified total RNA from blood
Mechanism of Action Enzymatic rRNA depletion, ligation-based addition of adapters and indexes Bead-based rRNA depletion, cDNA synthesis, and PCR
Method Metatranscriptome Sequencing, Whole-Transcriptome Sequencing Whole-Transcriptome Sequencing Whole-Transcriptome Sequencing
Multiplexing Up to 384 Unique Dual Indexes (UDIs) High-throughput kits: Prepare 96 indexes samples using either combinatorial dual indexes or unique dual indexes (available soon), Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together
Species Category Bacteria, Human, Mouse, Rat Human, Mouse, Rat Human, Mouse, Rat
Technology Sequencing Sequencing Sequencing

Method-Specific Workflow Example

 

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