The AmpliSeq for Illumina sequencing solution offers a highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds of genes in a single run. AmpliSeq for Illumina panels are optimized for clinical cancer research and genetic disease studies.
|TruSeq Custom Amplicon Low Input Kit||AmpliSeq for Illumina Custom DNA Panel||AmpliSeq for Illumina On-Demand|
|Assay Time||~6.5 hours||As low as 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)||As low as 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)|
|Content Specifications||Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb||Custom content of interest - up to 5 Mb||Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes|
|Hands-On Time||~3.5 hours||1.5 hours||1.5 hrs|
|Input Quantity||10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)||1–100 ng (10 ng recommended per pool)||1-100 ng DNA|
|Mechanism of Action||Probe hybridization, extension-ligation, and PCR||Multiplex PCR||Multiplex PCR|
|Method||Amplicon Sequencing , Custom Sequencing||Amplicon Sequencing , Custom Sequencing, Genotyping by Sequencing , Targeted DNA Sequencing||Amplicon Sequencing , Targeted DNA Sequencing|
|Multiplexing||1–96||Up to 96-plex||Up to 96-plex|
|Specialized Sample Types||FFPE Tissue||Blood, FFPE Tissue||FFPE Tissue|
|Species Category||Bovine, Human, Mouse, Other, Rat||Any Species, Bovine, Canine, Chicken, Hamster, Human, Maize, Mouse, Ovine, Porcine, Rice, Soybean, Tomato||Human|
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The TruSeq Custom Amplicon assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.
The TruSeq Custom Amplicon Low Input Library Prep Kit is part of an integrated, fully supported workflow for amplicon sequencing that guides researchers from design through data analysis. Illumina technical and field specialists help ensure rapid resolution and minimize potential laboratory downtime.
The TruSeq Custom Amplicon Low Input Library Prep Kit demonstrates high concordance between expected and observed variant frequencies for 10 ng high-quality FFPE DNA. Reference DNA samples were prepared following the TruSeq Custom Amplicon Low Input workflow with the TruSeq Amplicon - Cancer Panel primer pool, and sequenced on the MiSeq System in replicates of 4. Variants were called using MiSeq Reporter Software. R2 values are shown.
|Technology||TruSeq Custom Amplicon Low Input||TruSeq Custom Amplicon v1.5|
|Amplicon plexity (single pool)||Up to 1536||Up to 10,000 (via Concierge)|
|Input requirement||as low as 10 ng||50 ng|
|FFPE Compatibility||Yes – 10 ng (FFPE DNA input dependent on quality)||Yes – 250 ng|
|Amplicon size||150, 175, 250 bp||150, 175, 250, 425 bp|
|Instrument||MiniSeq, MiSeq, NextSeq||MiniSeq, MiSeq, NextSeq|
|Read Length||Up to 2 x 150 bp||Up to 2 x 300 bp|
|Time||6.5 hours||10 hours|
|Kit size (samples)||16, 96+||96+|
Two amplicon-based options for targeted sequencing are now available: TruSeq Custom Amplicon Low Input for low-input DNA and FFPE, or TruSeq Custom Amplicon 1.5 for very high plexity pools or longer amplicons.
TruSeq Custom Amplicon Low Input enables you to detect and discover high-quality information, even from limited and challenging samples. Use the table to compare assays