TruSeq DNA Exome

This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths. Read More...
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TruSeq Exome Kit (24 Samples)



TruSeq® Exome Kit (96 Samples)


Accessory Products

Illumina® Free Adapter Blocking Reagent (12 Reactions)



Illumina® Free Adapter Blocking Reagent (48 Reactions)



Product Highlights

The TruSeq Exome Kit, previously known as the TruSeq Exome Library Prep Kit, is a cost-effective library preparation and exome enrichment solution that offers:

  • Mechanical shearing and TruSeq enrichment technology that yield uniform coverage and high on-target sequencing reads
  • A fully supported workflow solution to simplify exome sequencing
Cost-Effective Exome Sequencing

The TruSeq Exome Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and variant identification by sequencing up to 12 libraries per flow cell lane. This enables sequencing of more exomes per run, so researchers can maximize their budgets.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Proven TruSeq Data Quality

The TruSeq Exome Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.

Convenient, Integrated Workflow Solution

Illumina provides an integrated, supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.

Find automation vendors with robotic systems compatible with this product

Illumina–IDT Exome Enrichment Solution

Illumina is partnering with IDT to co-market a combined exome enrichment solution using the xGen® Exome Research Panel v1.0, which consists of 429,826 individually synthesized and quality-controlled xGen Lockdown® Probes. The panel spans a 39 Mb target region (19,396 genes) of the human genome and covers 51 Mb of end-to-end tiled space. xGen Blocking Oligos (Cat. No.1075474, 1075475, or 1075476 for TruSeq libraries) and xGen Lockdown Reagents (Cat. No. 1072280 or 1072281) are also required.

Learn more about the IDT xGen Exome Research Panel
Illumina and IDT Partner on NGS Multiplexing and Exome Enrichment

Frequently Purchased Together


Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System Samples per run: mid output: 3, high output: 12 (based on 50 million reads, 50× coverage, 4 Gb of data) 2 × 75 bp (max recommended)
HiSeq 2500 System Samples per run (dual flow cell): rapid run: 24, high output: 156 (based on 50 million reads, 50× coverage, 4 Gb of data) 2 × 75 bp (max recommended)
NovaSeq 6000 System Samples per run (dual flow cell): S1: 160, S2: 320, S4: 769 (based on 50 million reads, 50× coverage, 4 Gb of data) ≤ 2 × 100 bp

Product Comparison

TruSeq DNA Exome Illumina DNA Prep with Enrichment
Assay Time 2.5 days ~6.5 hours
Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
Description A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.
Hands-On Time 6 hours ~2 hours
Input Quantity 100 ng genomic DNA 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).
Mechanism of Action Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes Bead-bound transposomes and hybrid-capture chemistry
Method Exome Sequencing Custom Sequencing, Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing
Multiplexing Up to 12-plex enrichment Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.
On-Target Reads >80% ≥85% (for exome panel)*
Specialized Sample Types FFPE Tissue Blood, FFPE Tissue, Saliva
Species Category Human Human, Other
Uniformity >85% (% coverage at 20x for 4 Gb) ≥90% (% coverage at 20x for 4 Gb; for exome panel)

Method-Specific Workflow Example


Customer Stories

Using Large Sample Sizes and Deep Sequencing to Find the Root Cause for Psychiatric Disorders

Dr. James Knowles trusts the HiSeq System and TruSeq library preparation kits for cost-effective, large-scale collaborative projects.

Learn More

Supporting Data and Figures

On-Target Exome Enrichment
Percentage of Reads on Target

The TruSeq Exome Kit delivers ≥80% of on-target sequencing reads for efficient, cost-effective exome sequencing.

TruSeq Exome Kit (Pre-Assembled Kits)
Catalog Number Total Samples Number of Enrichment Reactions Index Adapters
20020614 24 8 24 single indexes (Set A and Set B)
20020615 96 8 96 combinatorial dual indexes

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