The TruSeq Exome Kit, previously known as the TruSeq Exome Library Prep Kit, is a cost-effective library preparation and exome enrichment solution that offers:
The TruSeq Exome Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and variant identification by sequencing up to 12 libraries per flow cell lane. This enables sequencing of more exomes per run, so researchers can maximize their budgets.
To learn more about calculating coverage estimates, see the sequencing coverage calculator.
The TruSeq Exome Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.
Illumina provides an integrated, supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.
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Illumina is partnering with IDT to co-market a combined exome enrichment solution using the xGen® Exome Research Panel v1.0, which consists of 429,826 individually synthesized and quality-controlled xGen Lockdown® Probes. The panel spans a 39 Mb target region (19,396 genes) of the human genome and covers 51 Mb of end-to-end tiled space. xGen Blocking Oligos (Cat. No.1075474, 1075475, or 1075476 for TruSeq libraries) and xGen Lockdown Reagents (Cat. No. 1072280 or 1072281) are also required.Learn more about the IDT xGen Exome Research Panel
|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 550 System||Samples per run: mid output: 3, high output: 12 (based on 50 million reads, 50× coverage, 4 Gb of data)||2 × 75 bp (max recommended)|
|HiSeq 2500 System||Samples per run (dual flow cell): rapid run: 24, high output: 156 (based on 50 million reads, 50× coverage, 4 Gb of data)||2 × 75 bp (max recommended)|
|NovaSeq 6000 System||Samples per run (dual flow cell): S1: 160, S2: 320, S4: 769 (based on 50 million reads, 50× coverage, 4 Gb of data)||≤ 2 × 100 bp|
|TruSeq DNA Exome||Illumina DNA Prep with Enrichment|
|Assay Time||2.5 days||~6.5 hours|
|Content Specifications||Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).||Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
|Description||A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content.||A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.|
|Hands-On Time||6 hours||~2 hours|
|Input Quantity||100 ng genomic DNA||10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).|
|Mechanism of Action||Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes||Bead-bound transposomes and hybrid-capture chemistry|
|Method||Exome Sequencing||Custom Sequencing, Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing|
|Multiplexing||Up to 12-plex enrichment||Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.|
|On-Target Reads||>80%||≥85% (for exome panel)*|
|Specialized Sample Types||FFPE Tissue||Blood, FFPE Tissue, Saliva|
|Species Category||Human||Human, Other|
|Uniformity||>85% (% coverage at 20x for 4 Gb)||≥90% (% coverage at 20x for 4 Gb; for exome panel)|
Dr. James Knowles trusts the HiSeq System and TruSeq library preparation kits for cost-effective, large-scale collaborative projects.Learn More
The TruSeq Exome Kit delivers ≥80% of on-target sequencing reads for efficient, cost-effective exome sequencing.
|Catalog Number||Total Samples||Number of Enrichment Reactions||Index Adapters|
|20020614||24||8||24 single indexes (Set A and Set B)|
|20020615||96||8||96 combinatorial dual indexes|
Data Sheet | PDF | 5 versions
Illumina Exomes Comparative Table
Product Information Sheet | PDF < 1 MB
Evaluating DNA Quality from FFPE Samples
Technical Note | PDF < 1 MB
TruSeq DNA Exome Reference Guide Documentation
Sequencing Library qPCR Quantification Guide Documentation
TruSeq Exome Library Prep Checklist Documentation
TruSeq Exome Library Prep Reference Guide Documentation
TruSeq Exome Library Prep Consumables and Equipment List Documentation
TruSeq DNA Exome Consumables & Equipment Documentation
TruSeq DNA Exome Checklist Documentation
TruSeq DNA Exome Support - Documentation & Literature
Custom Protocol Selector
Generates customized, end-to-end instructions