Illumina DNA Prep with Exome 2.5 Enrichment

A high-performance, fast, and complete whole-exome sequencing kit including library prep and hybridization reagents, a comprehensive exome probe panel, clean up/size selection beads, and indexes. Read More...
Select Product(s)

Illumina DNA Prep with Exome 2.5 Enrichment, (S) Tagmentation Set B (96 Samples, 12-plex)

20077595

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Illumina DNA Prep with Exome 2.5 Enrichment, (S) Tagmentation Set D (96 Samples, 12-plex)

20077596

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Panel

Twist Bioscience for Illumina Mitochondrial Panel

20093180

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Accessory Products
What accessories do I need?

Flex Lysis Reagent Kit (96 reactions)

20018706

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Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20091654

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Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20091656

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Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20091658

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Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20091660

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Infinium MIDI Heatblock Insert

BD-60-601

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Illumina Purification Beads, 100ml

20060057

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Illumina Purification Beads, 400ml

20060058

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Product Highlights

Illumina DNA Prep with Exome 2.5 Enrichment offers a cost-effective, reliable solution for human whole-exome sequencing (WES).

Optional Mitochondrial Genome Enrichment

The Twist Bioscience for Illumina Mitochondrial Panel is a fixed content panel designed to cover the 16,659 base pairs (bp) and 37 genes of the mitochondrial genome (chrM) and allows enrichment and sequencing of mitochondrial DNA (mtDNA) variants. Use this panel as a spike-in with the Twist Bioscience for Illumina Exome 2.5 Panel and add coverage of ChrM to your experiments, with the same high WES on-target rates and coverage uniformity.

Outstanding Enrichment Performance

Illumina DNA Prep with Exome 2.5 Enrichment provides high on-target rates and uniform coverage enabling high multiplexing, in turn decreasing costs and increasing sample throughput.

Automation-friendly Workflow

Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.

Fast, Streamlined Workflow

Illumina DNA Prep with Exome 2.5 Enrichment uses a fast, user-friendly workflow with built-in library yield normalization and a simplified, single hybridization protocol. Go from DNA to library prep, enrichment, and post-enrichment amplification in just 6.5 hours.

Single-Vendor End-to-End Solution

You and your lab can benefit from a WES solution with support from a single, reliable partner—starting from direct blood or saliva input, through library prep, enrichment, sequencing, analysis, interpretation, and reporting.

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 2000 System Samples per run (by flow cell type): P2: 19, P3: 57 (based on 50× mean target coverage, 42M reads, 4.2 Gb of data) 2 × 101 bp
NovaSeq 6000 System Samples per run by flow cell type: SP: 34, S1: 69, S2: 176, S4: 428 based on 50× mean target coverage, 42M reads, 4.2 Gb of data. Note: S4: 428 requires NovaSeq Xp workflow or additional indexes. 2 × 101 bp

Product Comparison

Illumina DNA Prep with Exome 2.5 Enrichment Illumina DNA Prep with Enrichment
Assay Time 6.5 hours ~6.5 hours
Content Specifications Twist Bioscience for Illumina Exome 2.5 Panel
37.5 Mb coding content (≥ 99% of RefSeq, CCDS, ClinVar and ACMG pathogenic/likely pathogenic variants, COSMIC Cancer Gene Census), OMIM
Twist Bioscience for Illumina Mitochondrial Panel covers the circular mitochondrial genome ChrM (16,659 bp, 37 genes)
Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
Description A fast and easy-to-use library prep with enrichment workflow with a focused enrichment probe panel of up-to-date exome content for cost-effective and reliable human whole-exome sequencing. A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.
Hands-On Time ~2 hours ~2 hours
Input Quantity 50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide) 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).
Mechanism of Action Bead-linked transposome and hybrid-capture chemistry Bead-bound transposomes and hybrid-capture chemistry
Method Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing Custom Sequencing, Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing
Multiplexing Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible. Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.
Nucleic Acid Type DNA DNA
On-Target Reads ~ 90% (ave.) based on average performance from internal testing ≥85% (for exome panel)*
Specialized Sample Types Blood, Saliva Blood, FFPE Tissue, Saliva
Species Category Human Human, Other
System Compatibility HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000, NovaSeq X, NovaSeq X Plus HiSeq 3000, HiSeq 4000, iSeq 100, MiniSeq, MiSeq, MiSeqDx in Research Mode, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000, NovaSeq X, NovaSeq X Plus
Technology Sequencing Sequencing
Uniformity ≥ 91% (% coverage at 20× for 5 Gb) based on average performance from internal testing ≥90% (% coverage at 20x for 4 Gb; for exome panel)
Variant Class Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants

Case Studies

Tumor exomes reveal new insights into cancer biology

Dr. Paola Ulivi, Dr. Milena Urbini and Dr. Gianluca Tedali from the Translational Oncology Unit at IRST-IRCCS Bioscience Laboratory discuss how tumor exome sequencing enables a deeper understanding of the molecular landscape of cancers and guides biomarker discovery

Read Interview

Supporting Data and Figures

 

Manuals and Support Information

Custom Protocol Selector
Generates customized, end-to-end instructions

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