TruSeq DNA Exome offers two configuration options. The product previously known as the TruSeq Exome Library Prep Kit (Cat. No. FC-150-1001, FC-150-1002, FC-150-1003, and FC-150-1004) is now called the TruSeq Exome Kit and is available as a pre-assembled kit. Kits include all Illumina reagents for library preparation, index adapters, enrichment reagents, and an exome panel. To enable the combination of the Illumina TruSeq DNA library preparation with IDT’s xGen exome enrichment, major components (such as library preparation and index adapters) can also be purchased separately. A full list of components available for individual purchase can be found on the “Choose Your Components” tab. Reagents required for IDT’s xGen exome enrichment can be purchased by contacting IDT.

TruSeq DNA Exome

This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths. Read More...
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TruSeq Exome Kit (24 Samples)



TruSeq Exome Kit (96 samples)


Library Prep

TruSeq DNA Library Prep for Enrichment (24 Samples)



TruSeq DNA Library Prep for Enrichment (96 Samples)



Index Adapters

TruSeq DNA CD Indexes (96 Indexes, 96 Samples)



TruSeq DNA Single Indexes Set A (12 Indexes, 24 Samples)



TruSeq DNA Single Indexes Set B (12 Indexes, 24 Samples)



IDT for Illumina – TruSeq DNA UD Indexes (24 Indexes, 96 Samples)



IDT for Illumina – TruSeq DNA UD Indexes (96 Indexes, 96 Samples)



Product Highlights

The TruSeq Exome Kit, previously known as the TruSeq Exome Library Prep Kit, is a cost-effective library preparation and exome enrichment solution that offers:

  • Modular product ordering for low-cost exome sequencing
  • Mechanical shearing and TruSeq enrichment technology that yield uniform coverage and high on-target sequencing reads
  • A fully supported workflow solution to simplify exome sequencing
Cost-Effective Exome Sequencing

The TruSeq Exome Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and variant identification by sequencing up to 12 libraries per flow cell lane. This enables sequencing of more exomes per run, so researchers can maximize their budgets.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Proven TruSeq Data Quality

The TruSeq Exome Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.

Convenient, Integrated Workflow Solution

Illumina provides an integrated, supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.

Illumina now offers modular product ordering to enable flexibility in your workflows.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this workflow

Illumina–IDT Exome Enrichment Solution

Illumina is partnering with IDT to co-market a combined exome enrichment solution using the xGen® Exome Research Panel v1.0, which consists of 429,826 individually synthesized, and quality controlled xGen Lockdown® Probes. The panel spans a 39 Mb target region (19,396 genes) of the human genome and covers 51 Mb of end-to-end tiled space. xGen Blocking Oligos (Cat. No.1075474, 1075475, or 1075476 for TruSeq libraries; contact IDT for Nextera libraries), and xGen Lockdown Reagents (Cat. No. 1072280 or 1072281) are also required

Learn more about the IDT xGen Exome Research Panel v1.0
Illumina and IDT Partner on NGS Multiplexing and Exome Enrichment


Method-Specific Workflow Example


Customer Stories

Using Large Sample Sizes and Deep Sequencing to Find the Root Cause for Psychiatric Disorders

Dr. James Knowles trusts the HiSeq System and TruSeq library preparation kits for cost-effective, large-scale collaborative projects.

Learn More

Supporting Data and Figures

On-Target Exome Enrichment
Percentage of Reads on Target

The TruSeq Exome Kit delivers ≥80% of on-target sequencing reads for efficient, cost-effective exome sequencing.

TruSeq Exome Kit (Pre-Assembled Kits)
Catalog Number Total Samples Number of Enrichment Reactions Index Adapters
20020614 24 8 24 single indexes (Set A and Set B)
20020615 96 8 96 combinatorial dual indexes

Product Literature

Evaluating DNA Quality from FFPE Samples

Technical Note | PDF < 1 MB

Exomes Comparative Table,Illumina Exomes Comparative Table

Product Information Sheet | PDF < 1 MB

TruSeq DNA Exome

Data Sheet | PDF < 1 MB

Manuals and Support Information

TruSeq DNA Exome Enrichment Workflow Documentation

TruSeq DNA Exome Support - Documentation & Literature

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq DNA Exome Support 

All TruSeq DNA Exome Enrichment Workflow Support 

Related Products

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Nextera DNA Exome

This exome sequencing library preparation solution uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome.

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Nextera Rapid Capture Custom Enrichment Kit

Custom target enrichment kit to rapidly interrogate those portions of the human genome most important to your specific research.

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TruSight One Sequencing Panels

Comprehensive panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.