TruSeq Exome

This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths. Read More...
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TruSeq® Exome Kit (8 rxn × 3 plex)



TruSeq® Exome Kit (8 rxn × 6 plex)



TruSeq® Exome Kit (8 rxn × 9 plex)



TruSeq® Exome Kit (8 rxn × 12plex)



Product Highlights

TruSeq Exome Library Prep Kit is a cost-effective library preparation and exome enrichment solution with industry-leading accuracy. It offers:

  • Pre-enrichment library pooling and optimal coverage for low-cost exome sequencing
  • Mechanical shearing and TruSeq enrichment technology that yield uniform coverage and greater than or equal to 80% on-target sequencing reads
  • Fully supported workflow solution to simplify exome sequencing
Cost-Effective Exome Sequencing

The TruSeq Exome Library Prep Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and identify variants in less time by sequencing up to 12 libraries per flow cell lane. The high on-target percentage requires fewer sequencing cycles to reach the optimal level of coverage, while still achieving high coverage uniformity for confident results. It also enables sequencing of more exomes per run, so researchers can maximize their budgets.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Proven TruSeq Data Quality

The TruSeq Exome Library Prep Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Library Prep Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.

Convenient, Integrated Workflow Solution

Illumina provides an integrated, fully supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit


Method-Specific Workflow Example


Customer Stories

Using Large Sample Sizes and Deep Sequencing to Find the Root Cause for Psychiatric Disorders

Dr. James Knowles trusts the HiSeq System and TruSeq library preparation kits for cost-effective, large-scale collaborative projects.

Learn More

Supporting Data and Figures

On-Target Exome Enrichment
Percentage of Reads on Target

The TruSeq Exome Library Prep Kit delivers ≥80% of on-target sequencing reads for efficient, cost-effective exome sequencing.

TruSeq Exome Library Prep Kit Configurations
Catalog Number TruSeq Exome Library Prep Kit Total Samples Number of Enrichment Reactions Number of Samples per Enrichment Reaction Unique Index Adapter Combinations
FC-150-1001 8 rxn × 3 plex 24 8 3 3
FC-150-1002 8 rxn × 6 plex 48 8 6 6
FC-150-1003 8 rxn × 9 plex 72 8 9 9
FC-150-1004 8 rxn × 12 plex 96 8 12 96

Product Literature

Evaluating DNA Quality from FFPE Samples

Technical Note | PDF(< 1 MB)

Illumina Exomes Comparative Table

Product Information Sheet | PDF(< 1 MB)

TruSeq Exome Library Prep Kit

Data Sheet | PDF(< 1 MB)

Manuals and Support Information

TruSeq Exome Library Prep Kit Documentation  

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq Exome Library Prep Kit Support 

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