|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 550 System||Samples per run: mid output: 3, high output: 12 (based on 50 million reads, 50× coverage, 4 Gb of data)||2 × 75 bp (max recommended)|
|HiSeq 2500 System||Samples per run (dual flow cell): rapid run: 24, high output: 156 (based on 50 million reads, 50× coverage, 4 Gb of data)||2 × 75 bp (max recommended)|
|NovaSeq 6000 System||Samples per run (dual flow cell): S1: 160, S2: 320, S4: 769 (based on 50 million reads, 50× coverage, 4 Gb of data)||≤ 2 × 100 bp|
|TruSeq DNA Exome||Nextera DNA Exome||AmpliSeq for Illumina Exome Panel||Nextera Flex for Enrichment|
|Assay Time||2.5 days||< 2.5 days||< 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)||~6.5 hours|
|Content Specifications||Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).||Captures coding exons. Spans a 39 Mb target region and covers 51 Mb of end-to-end tiled space.||> 97% of CCDS||Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
|Description||A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content.||A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content.||Enables researchers to prepare 8 exome libraries in <7.5 hours with <1.5 hours hands-on time.||A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.|
|Hands-On Time||6 hours||3 hours||< 1.5 hours||~2 hours|
|Input Quantity||100 ng genomic DNA||50 ng genomic DNA||50 ng minimum||10-1000 ng high-quality genomic DNA or ≥ 50 ng FFPE DNA. (For blood and saliva, see the reference guide).|
|Mechanism of Action||Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes||Transposase-based fragmentation and exome enrichment with biotinylated capture probes||Multiplex PCR||Enrichment-compatible, bead-linked transposome|
|Method||Exome Sequencing||Exome Sequencing||Amplicon Sequencing , Exome Sequencing||Custom Sequencing, Exome Sequencing , Target Enrichment , Target Enrichment, Targeted DNA Sequencing|
|Multiplexing||Up to 12-plex enrichment||Up to 12-plex enrichment||96 dual index combinations||Up to 96 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.|
|On-Target Reads||>80%||>75%||>80%||≥85% (for exome panel)|
|Specialized Sample Types||FFPE Tissue||Low-Input Samples, Not FFPE-Compatible||Not FFPE-Compatible||Blood, FFPE Tissue, Saliva|
|Species Category||Human||Human||Human||Human, Other|
|Uniformity||>85% (% coverage at 20x for 4 Gb)||>75% (% coverage at 20x for 4 Gb)||>90% (% coverage at 20x for 4 Gb)||≥90% (% coverage at 20x for 4 Gb; for exome panel)|
Dr. James Knowles trusts the HiSeq System and TruSeq library preparation kits for cost-effective, large-scale collaborative projects.Learn More
The TruSeq Exome Kit delivers ≥80% of on-target sequencing reads for efficient, cost-effective exome sequencing.
|Catalog Number||Total Samples||Number of Enrichment Reactions||Index Adapters|
|20020614||24||8||24 single indexes (Set A and Set B)|
|20020615||96||8||96 combinatorial dual indexes|