Reagents provided in the Illumina Ribo-Zero Plus rRNA Depletion kit remove abundant RNA using enzymatic depletion. The remaining RNA is then converted into sequencing-ready libraries for Illumina sequencing systems using TruSeq Stranded Total RNA reagents.
| Sample | rRNAs Targeted |
|---|---|
| Human cytoplasmic rRNAs | 28S, 18S, 5.8S, 5S |
| Human mitochondrial rRNAs | 12S, 16S |
| Human beta globin transcripts | HBA1, HBA2, HBB, HBG1, HBG2 |
| Mouse & Rat rRNA | 16S, 28S |
| Gram(-) Bacterial rRNAs | E. coli: 5S, 16S, 23S |
| Gram(+) Bacterial rRNAs | Bacillus subtilis: 5S, 16S, 23S |
| Ribo-Zero Plus Microbiome rRNA Depletion Kit | Ribo-Zero Plus rRNA Depletion Kit | |
|---|---|---|
| Description | Depletion of abundant transcripts from host and bacterial rRNA for metatranscriptomics research. | Single-tube depletion of abundant transcripts from multiple species or transcripts |
| Input Quantity | Minimum 25 ng of total RNA, 50 ng recommended for optimal performance | 1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples |
| System Compatibility Details | Library prep is designed to be compatible with all lllumina sequencing systems, and is recommended to be used with NextSeq 500, NextSeq 550, NextSeq 1000, NextSeq 2000, and NovaSeq 6000 Systems. | Library prep is designed to be compatible with all lllumina sequencing systems, and is extensively validated on the NextSeq 500, NextSeq 550 and NovaSeq 6000 Systems. |
| Strand Specificity | Stranded | Stranded |
| Method | Whole-Transcriptome Sequencing, Metatranscriptome Sequencing | Whole-Transcriptome Sequencing |
| Variant Class | Novel Transcripts, Transcript Variants | Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants |
| Species Category | Bacteria, Human, Mouse, Rat | Bacteria, Human, Mouse, Rat |
| Species Details | Depletion of abundant rRNA transcripts from complex bacterial (microbiome) samples including stool samples, in addition to all transcripts listed in Ribo-Zero Plus. | Depletion of abundant transcripts from multiple species including: human cytoplasmic & mitochondria rRNA, mouse rRNA, rat rRNA, E. coli and B. subtilis rRNA, human beta globin transcripts |
| System Compatibility | NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000 | HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000 |
| Specialized Sample Types | Complex microbial samples (ie. stool) | FFPE Tissue, Low-Input Samples |
| Nucleic Acid Type | RNA | RNA |
Microbial RNA sequencing enabled with the Illumina Ribo-Zero Plus rRNA Depletion Kit
Technical Note | PDF < 1 MB
Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus
Data Sheet | PDF 1 MB
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A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
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