Reagents provided in the Illumina Ribo-Zero Plus rRNA Depletion kit remove abundant RNA using enzymatic depletion. The remaining RNA is then converted into sequencing-ready libraries for Illumina sequencing systems using TruSeq Stranded Total RNA reagents.
| Sample | rRNAs Targeted |
|---|---|
| Human cytoplasmic rRNAs | 28S, 18S, 5.8S, 5S |
| Human mitochondrial rRNAs | 12S, 16S |
| Human beta globin transcripts | HBA1, HBA2, HBB, HBG1, HBG2 |
| Mouse & Rat rRNA | 16S, 28S |
| Gram(-) Bacterial rRNAs | E. coli: 5S, 16S, 23S |
| Gram(+) Bacterial rRNAs | Bacillus subtilis: 5S, 16S, 23S |
Microbial RNA sequencing enabled with the Illumina Ribo-Zero Plus rRNA Depletion Kit
Technical Note | PDF < 1 MB
Data Sheet | PDF | 6 versions
Infinium LiHa and Infinium RoMa Operating Manual Documentation
Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility.
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.
Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. Remove rRNA from plant leaf, seed, and root tissue.
