Reagents provided in the Illumina Ribo-Zero Plus rRNA Depletion kit remove abundant RNA using enzymatic depletion. The remaining RNA is then converted into sequencing-ready libraries for Illumina sequencing systems using TruSeq Stranded Total RNA reagents.
Sample | rRNAs Targeted |
---|---|
Human cytoplasmic rRNAs | 28S, 18S, 5.8S, 5S |
Human mitochondrial rRNAs | 12S, 16S |
Human beta globin transcripts | HBA1, HBA2, HBB, HBG1, HBG2 |
Mouse & Rat rRNA | 16S, 28S |
Gram(-) Bacterial rRNAs | E. coli: 5S, 16S, 23S |
Gram(+) Bacterial rRNAs | Bacillus subtilis: 5S, 16S, 23S |
Microbial RNA sequencing enabled with the Illumina Ribo-Zero Plus rRNA Depletion Kit
Technical Note | PDF< 1 MB
Data Sheet | PDF | 6 versions
Infinium LiHa and Infinium RoMa Operating Manual Documentation