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TruSight RNA Pan-Cancer Panel

Targeting 1385 cancer genes for gene expression, variant, and fusion detection studies in many RNA sample types including blood, bone marrow, and formalin-fixed, paraffin-embedded (FFPE) tissue.Read More...
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TruSight RNA Pan-Cancer Panel Set A

RS-303-1002

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TruSight RNA Pan-Cancer Panel Set B

RS-303-1003

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TruSight RNA Pan-Cancer Set A MiniSeq Kit

20005611

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Product Highlights

The TruSight RNA Pan-Cancer panel provides a comprehensive analysis of the cancer transcriptome. It offers:

  • Gene expression information, variant calling, and fusion detection with known and novel gene fusion partners
  • Optimized, low-input protocol for a wide range of sample types including FFPE
  • A comprehensive view of cancer pathways
  • Economical RNA sequencing (RNA-Seq) on a desktop sequencer
Highly Sensitive and Economical Targeted Sequencing

The TruSight RNA Pan-Cancer panel enables the quantitative measurement of gene expression as well as the detection of gene fusions with both known and novel gene fusion partners. The panel accommodates as little as 10 ng of total RNA input (or 20 ng from FFPE samples). The focus on a subset of relevant genes enables RNA-Seq with high sensitivity at 8 samples per run on a desktop sequencer, allowing cost-effective access to NGS for any lab.

Intuitive Cloud-based Data Analysis

Analysis can be performed using the RNA-Seq Alignment BaseSpace App. This intuitive tool performs fusion calling andvariant detection, and provides gene expression profiles, offering a comprehensive solution to all cancer researchers.

TruSight RNA Pan-Cancer Sample Datasets
MiSeq Data

10 ng of human reference RNA (UHR), human reference brain RNA (Brain), cell line RNA (MCF7), and 20 ng of breast tumor FFPE RNA (BT) were prepared using the TruSight RNA Pan-Cancer Panel and sequenced on the MiSeq System. Read mapping and fusion calling were performed using the RNA-Seq Alignment App with STAR aligner on BaseSpace Sequence Hub.

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MiniSeq Data

Human reference RNA (HBRR and UHRR), cell line RNA (MCF7), and breast tumor RNA samples were prepared using the TruSight RNA Pan-Cancer panel, and 8 samples were sequenced on the MiniSeq System at a 2x76bp read length with single indexing. The total yield was 4.2 Gb with 96.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

Access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiniSeq System Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample) 2 × 75 bp (max recommended)
MiSeq System 8 samples per run with v3 reagents (based on 3 million reads per sample) 2 × 75 bp (max recommended)

Product Comparison

TruSight RNA Pan-Cancer TruSight RNA Fusion Panel
Assay Time 2.5 days 2.5 days
Cancer Type Pan-Cancer Pan-Cancer, Solid Tumor
Content Specifications Pan-cancer panel with 1,385 targeted genes and 21,043 targeted exonic regions. Includes 507 genes involved in cancer gene fusions. Gene fusion panel targeting 507 cancer-associated fusion genes and 7690 exons
Description Comprehensive assessment of cancer-related RNA transcripts that enables research on gene fusions, variants, and gene expression changes in multiple cancer types. Enables gene fusion detection studies with known and novel partners in 507 fusion-associated genes, with a simple analysis solution.
Hands-On Time 11 hours 11 hours
Input Quantity 10 ng total RNA, 20 - 100 ng FFPE RNA 10 ng total RNA, 20–100 ng FFPE RNA
Specialized Sample Types FFPE Tissue, Low-Input Samples FFPE Tissue, Low-Input Samples
Species Category Human Human

Method-Specific Workflow Example

 

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