Robust cfDNA liquid biopsy NGS assay, interrogating 34 genes from plasma across multiple solid tumor types.
Assay time
Hands-on time
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The Pillar oncoReveal Essential LBx Panel is a robust NGS assay designed to interrogate 200 targets from 34 genes relevant across multiple solid tumor types using cell-free DNA (cfDNA) extracted from plasma.
This focused panel can detect four types of variants from cfDNA extracted from plasma: single nucleotide variants (SNVs), small insertion/deletion (indel) variants, copy number amplification (CNA), and microsatellite instability (MSI).
The assay uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.
The workflow allows labs to maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab.
| Assay time | 48 hours from sample to report |
|---|---|
| Cancer type | Pan-cancer, Solid tumor |
| Content specifications | 200 amplicons across 34 genes |
| Description | The Pillar oncoReveal Essential LBx Panel is a robust cfDNA liquid biopsy NGS assay that interrogates 200 targets from 34 genes of interest across multiple solid tumor types. |
| Hands-on time | ~3 hr |
| Input quantity | 10–30 ng cfDNA |
| Instruments | NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, MiSeq i100 System |
| Mechanism of action | Multiplex PCR using tiled amplicon |
| Method | Targeted DNA sequencing, Amplicon sequencing |
| Nucleic acid type | DNA |
| Number of amplicons | Pool 1: 200 amplicons |
| Number of reactions | 24 reactions per kit |
| On-target reads | ≥ 90% |
| Specialized sample types | Cell-free DNA |
| Species category | Human |
| Target insert size | 90 bp |
| Technology | Sequencing |
| Uniformity | ≥ 90% |
| Variant class | Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs), Blood microsatellite instability (bMSI) |
Pillar oncoReveal Essential LBx Panel also requires the use of a Pillar index kit, sequencing reagents, and PiVAT secondary analysis software. If additional variant interpretation is desired, Illumina Connected Insights is recommended.
Pillar oncoReveal Essential LBx Panel analyzes multiple solid tumor types using cell-free DNA (cfDNA) extracted from plasma in under 8 hours of sample processing time with approximately 3 hours of hands-on time.
Pillar oncoReveal Essential LBx Panel
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Advancing cancer research through NGS
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A dedicated support section is not currently available for this product
| Pillar oncoReveal Essential LBx Panel | TruSight Oncology 500 ctDNA v2 | Pillar oncoReveal Core LBx Panel | Pillar oncoReveal Fusion LBx Panel | |
|---|---|---|---|---|
| Assay time | 48 hours from sample to report | 3-4 days from purified nucleic acid to variant report | 48 hours from sample to report | 48 hours from sample to report |
| Content specifications | 200 amplicons across 34 genes |
Targeted selection of 523 genes (full coding sequence) for a total of 1.94 Mb panel size. • Immuno-oncology biomarker coverage: TMB and MSI • Guideline coverage: Broad coverage of key guidelines for multiple solid tumor types • Clinical trial coverage: Over 600 clinical trials (based on Velsera Knowledgebase, as of February 2023) |
446 amplicons across 104 genes | 18 driver genes and 83 partners |
| Description | The Pillar oncoReveal Essential LBx Panel is a robust cfDNA liquid biopsy NGS assay that interrogates 200 targets from 34 genes of interest across multiple solid tumor types. | Provides a noninvasive research method for comprehensive genomic profiling of liquid biopsy samples (ctDNA from blood plasma). This liquid biopsy approach provides insights about intra- and inter-tumor heterogeneity using a minimally invasive sample collection approach to complement tissue-based CGP. | The Pillar oncoReveal Core LBx Panel is a robust cfDNA liquid biopsy NGS assay that interrogates 446 targets from 104 genes of interest across multiple solid tumor types. | The Pillar oncoReveal Fusion LBx Panel is a robust cfRNA liquid biopsy NGS assay that interrogates fusion transcripts from 18 driver genes with more than 80 partners relevant across multiple solid tumor types. |
| Hands-on time | ~3 hr |
~1.5 hr for automated workflow ~2.5 hr for manual workflow |
~3 hr | ~3 hr |
| Input quantity | 10–30 ng cfDNA | 20 ng cfDNA (4 ml of plasma) | 10–30 ng cfDNA | 10–30 ng cfRNA |
| Method | Targeted DNA sequencing, Amplicon sequencing | Targeted DNA sequencing, Target enrichment | Targeted DNA sequencing, Amplicon sequencing | Amplicon sequencing, Targeted RNA sequencing |
| Nucleic acid type | DNA | DNA | DNA | RNA |
| Number of reactions | 24 reactions per kit | 24 reactions per kit | 24 reactions per kit | |
| On-target reads | ≥ 90% | ≥ 90% | ||
| Specialized sample types | Cell-free DNA | Circulating tumor DNA, Blood | Cell-free DNA | Cell-free RNA |
| Species category | Human | Human | Human | Human |
| Technology | Sequencing | Sequencing | Sequencing | Sequencing |
Library Prep and Array Kit Selector
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Gene List
Pillar oncoReveal Essential LBx includes 200 amplicons across 34 driver genes.
Panel Specifications
Pillar oncoReveal Essential LBx specifications for cfDNA interrogation from blood plasma.
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Robust cfDNA liquid biopsy NGS assay, interrogating 104 genes from plasma across multiple solid tumor types.
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