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The TruSight RNA Pan-Cancer panel provides a comprehensive analysis of the cancer transcriptome. It offers:
The TruSight RNA Pan-Cancer panel enables the quantitative measurement of gene expression as well as the detection of gene fusions with both known and novel gene fusion partners. The panel accommodates as little as 10 ng of total RNA input (or 20 ng from FFPE samples). The focus on a subset of relevant genes enables RNA-Seq with high sensitivity on a benchtop sequencer, allowing cost-effective access to NGS for any lab.
Analysis can be performed using the BaseSpace RNA-Seq Alignment App. This intuitive tool performs fusion calling and variant detection, and provides gene expression profiles, offering a comprehensive solution to cancer researchers.
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10 ng of human reference RNA (UHR), human reference brain RNA (Brain), cell line RNA (MCF7), and 20 ng of breast tumor FFPE RNA (BT) were prepared using the TruSight RNA Pan-Cancer Panel and sequenced on the MiSeq System. Read mapping and fusion calling were performed using the RNA-Seq Alignment App with STAR aligner on BaseSpace Sequence Hub.
Human reference RNA (HBRR and UHRR), cell line RNA (MCF7), and breast tumor RNA samples were prepared using the TruSight RNA Pan-Cancer panel, and 8 samples were sequenced on the MiniSeq System at a 2x76bp read length with single indexing. The total yield was 4.2 Gb with 96.8% of bases at or above Q30.
|Instrument||Recommended Number of Samples||Read Length|
|MiniSeq System||Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample)||2 × 75 bp (max recommended)|
|MiSeq System||8 samples per run with v3 reagents (based on 3 million reads per sample)||2 × 75 bp (max recommended)|
|TruSight RNA Pan-Cancer||TruSight RNA Fusion Panel|
|Assay Time||2.5 days||2.5 days|
|Cancer Type||Pan-Cancer||Pan-Cancer, Solid Tumor|
|Content Specifications||Pan-cancer panel with 1,385 targeted genes and 21,043 targeted exonic regions. Includes 507 genes involved in cancer gene fusions.||Gene fusion panel targeting 507 cancer-associated fusion genes and 7690 exons|
|Description||Comprehensive assessment of cancer-related RNA transcripts that enables research on gene fusions, variants, and gene expression changes in multiple cancer types.||Enables gene fusion detection studies with known and novel partners in 507 fusion-associated genes, with a simple analysis solution.|
|Hands-On Time||11 hours||11 hours|
|Input Quantity||10 ng total RNA, 20 - 100 ng FFPE RNA||10 ng total RNA, 20–100 ng FFPE RNA|
|Specialized Sample Types||FFPE Tissue, Low-Input Samples||FFPE Tissue, Low-Input Samples|
Evaluating RNA Quality from FFPE Samples
Technical Note | PDF 1 MB
Data Sheet | PDF < 1 MB
TruSight RNA Pan-Cancer Sequencing Panel Gene List
Product Information Sheet | EXCEL < 1 MB
TruSight RNA Pan-Cancer Panel Documentation
Illumina Experiment Manager User Guide Documentation
Sequencing Library qPCR Quantification Guide Documentation
Custom Protocol Selector
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