TruSeq Custom Amplicon v1.5

Focus on key genomic regions of interest with this targeted resequencing solution, useful for very high plexity pools or longer amplicons. Read More...
Select Product(s)

TruSeq Custom Amplicon Kit (96 samples)

FC-130-1001

Accessory Products
What accessories do I need?

TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples)

FC-130-1003

Price
 
 

TruSeq Custom Amplicon Filter Plate (1 plate)

FC-130-1006

Price
 
 

TruSeq Index Plate Fixture & Collar Kit (2 each)

FC-130-1007

Price
 
 

Infinium FFPE QC Kit (384 reactions)

WG-321-1001

Price
 
 

Product Highlights

TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. The assay enables you to focus interrogation on key regions of genomic interest. Up to 1536 amplicons can be sequenced in a single reaction using a simple workflow. This highly targeted approach offers unparalleled efficiency for discovering, validating, and screening genetic variants in areas of research focus.

Requiring as little as 50 ng of input genomic DNA (gDNA), this kit can be used with a wide range of sample types. The ability to multiplex up to 1536 amplicons per reaction allows coverage of up to 650 kb of cumulative sequence. Further, an integrated dual indexing scheme supports up to 96 samples per run, enabling you to fully benefit from desktop sequencer output capabilities.

This assay provides improved performance in difficult to address GC-rich regions, as well as increased library yield, uniformity, and stability. Additionally, optimized amplification steps enable sample input requirements to drop from 150 ng to 50 ng.

Simplified Probe Design

Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.

Learn more about DesignStudio
Start a project in DesignStudio now (login required)

Simple Data Analysis

Automated data analysis using the BaseSpace TruSeq Amplicon App allows researchers to easily review project data, including variants detected, across multiple runs.

Species Compatibility

TruSeq Custom Amplicon supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition, Illumina Concierge services can design custom assays and/or provide assay optimization support for any species of interest.

Inquire about Illumina Concierge services

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit

Specifications

Method-Specific Workflow Example

 

Customer Stories

Personalized Cancer Therapies through Precision Diagnostics

Dr. Robert Daber uses TruSeq Custom Amplicon to conduct genomic testing that can inform prognosis and therapeutic choices.

Learn More
Restoring and Managing Historical Columbia River Basin Fish Populations with Genomics

Novel GT-Seq method using the HiSeq System reduces genotyping costs and enables analysis of tens of thousands of fish.

Learn More

Supporting Data and Figures

 
Comparison of TruSeq Custom Amplicon Kits
Technology TruSeq Custom Amplicon v1.5 TruSeq Custom Amplicon Low Input
Amplicon plexity (single pool) Up to 10,000 (via Concierge) Up to 1536
Input requirement 50 ng as low as 10 ng
FFPE Compatibility Yes – 250 ng Yes – 10 ng (FFPE DNA input dependent on quality)
Amplicon size 150, 175, 250, 425 bp 150, 175, 250 bp
Instrument MiniSeq, MiSeq, NextSeq MiniSeq, MiSeq, NextSeq
Read Length Up to 2 x 300 bp Up to 2 x 150 bp
Time 10 hours 6.5 hours
Kit size (samples) 96+ 16, 96+

Two amplicon-based options for targeted sequencing are now available: TruSeq Custom Amplicon Low Input for low-input DNA and FFPE, or TruSeq Custom Amplicon 1.5 for very high plexity pools or longer amplicons.

TruSeq Custom Amplicon Low Input enables you to detect and discover high-quality information, even from limited and challenging samples. Use the table to compare assays

Product Literature

TruSeq Custom Amplicon v1.5

Data Sheet | PDF < 1 MB

Manuals and Support Information

TruSeq Custom Amplicon Support Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq Custom Amplicon Support 

Related Products

Image Source
TruSeq Custom Amplicon v1.5

Focus on key genomic regions of interest with this targeted resequencing solution, useful for very high plexity pools or longer amplicons.


Image Source
Nextera XT DNA Library Preparation Kit

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in less than 90 minutes, with a low DNA input requirement.