The AmpliSeq for Illumina sequencing solution offers a highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds of genes in a single run. AmpliSeq for Illumina panels are optimized for clinical cancer research and genetic disease studies.
Dr. Robert Daber uses TruSeq Custom Amplicon to conduct genomic testing that can inform prognosis and therapeutic choices.Learn More
Novel GT-Seq method using the HiSeq System reduces genotyping costs and enables analysis of tens of thousands of fish.Learn More
The TruSeq Custom Amplicon assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.
|Technology||TruSeq Custom Amplicon v1.5||TruSeq Custom Amplicon Low Input|
|Amplicon plexity (single pool)||Up to 10,000 (via Concierge)||Up to 1536|
|Input requirement||50 ng||as low as 10 ng|
|FFPE Compatibility||Yes – 250 ng||Yes – 10 ng (FFPE DNA input dependent on quality)|
|Amplicon size||150, 175, 250, 425 bp||150, 175, 250 bp|
|Instrument||MiniSeq, MiSeq, NextSeq||MiniSeq, MiSeq, NextSeq|
|Read Length||Up to 2 x 300 bp||Up to 2 x 150 bp|
|Time||10 hours||6.5 hours|
|Kit size (samples)||96+||16, 96+|
Two TruSeq Custom Amplicon options for targeted sequencing are available: TruSeq Custom Amplicon Low Input for low-input DNA and FFPE, or TruSeq Custom Amplicon 1.5 for very high plexity pools or longer amplicons. TruSeq Custom Amplicon Low Input enables you to detect and discover high-quality information, even from limited and challenging samples. Use the table to compare assays.