Illumina no longer accepts orders for the NeoPrep System. NeoPrep kits will be available to order until October 31, 2017. For alternative solutions, see our library prep automation page. Illumina remains committed to providing you with high-quality support and service.

TruSeq Nano DNA Library Prep Kit for NeoPrep

DNA library preparation for use with the NeoPrep System, providing comprehensive genomic coverage with low DNA input and minimal hands-on time. Read More...
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TruSeq Nano DNA Library Prep Kit for NeoPrep (16 indexes, 16 samples)



Product Highlights

The TruSeq Nano DNA for NeoPrep offers a simple, all-inclusive prep solution, ideal for any whole-genome sequencing application. Run the same proven TruSeq Nano DNA biochemistry on the fully integrated digital microfluidics NeoPrep Library Prep System.

  • Simplified and seamless workflow solution – Includes onboard quantification and normalization to deliver sequencing-ready libraries
  • Exceptional performance and reproducibility – Generates libraries with TruSeq Nano DNA coverage and quality, with minimal hands-on time and reduced user variability
  • Low input requirement – Requires as little as 25 ng genomic DNA to prepare high-quality, sequencing-ready libraries
Reduce Library Bias and Coverage Gaps

TruSeq Nano DNA library prep reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. It also provides excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables you to access more information from each sequencing run.

NeoPrep System enhances TruSeq Nano DNA performance

The NeoPrep System enhances TruSeq Nano DNA performance by providing high-quality reproducible results, even with low input amounts of DNA. Digital microfluidics technology precisely manipulates droplets that perform the library prep workflow, including quantification and normalization within the tightly controlled environment of the NeoPrep library card.

A simple, intuitive workflow delivers 16 sequencing-ready libraries, eliminating almost all manual steps, and reducing hands-on time from ~4 hours to just 30 minutes. In addition, digital microfluidics requires less DNA input, enabling excellent performance from 25-75 ng of genomic DNA. Successful libraries have been demonstrated with DNA inputs ranging from 1–100 ng, up to 10-fold lower than required by manual protocols.


Method-Specific Workflow Example


Customer Stories

Unmasking the Viral Etiology of Cancer and Immune Disease

Karolinska Institutet researchers use Illumina sequencers to identify novel HPV types associated with non-melanoma skin cancers.

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BRC-Seq Customers Benefit from Streamlined BaseSpace Integrated Workflow

BaseSpace enables tracking of samples, from library prep to data analysis, helping BRC-Seq deliver high-quality sequencing data and additional value to its customers.

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Product Literature

Manuals and Support Information

TruSeq Nano DNA Library Prep Kit for NeoPrep Support Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq Nano DNA Library Prep Kit for NeoPrep Support 

Other Kit Configurations

TruSeq Nano DNA Library Prep Kit variations are available to accommodate a range of study designs: the manual TruSeq Nano DNA LT Library Preparation Kit for low-throughput studies, and the liquid handling robot-automatable TruSeq Nano DNA HT Library Preparation Kit for high-throughput studies.

View Other Kit Configurations

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