Illumina no longer accepts orders for the NeoPrep System. NeoPrep kits will be available to order until October 31, 2017. For alternative solutions, see our library prep automation page. Illumina remains committed to providing you with high-quality support and service.

TruSeq Stranded mRNA Library Prep Kit for NeoPrep

Complete view of the transcriptome with walk-away workflow, lower input requirement, and excellent reproducibility. Read More...
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TruSeq Stranded mRNA Library Prep Kit for NeoPrep (16 samples, 24 indexes)



Product Highlights

The TruSeq Stranded mRNA Library Prep Kit for NeoPrep offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It enables robust interrogation of both standard and low-quality samples.

  • Simplified and seamless workflow solution – Generate libraries with minimal hands-on time
  • Unparalleled performance and reproducibility – Interrogate gene expression with TruSeq Stranded mRNA coverage and quality, and reduced user variability
  • Low input requirement – Begin library prep with as little as 25 ng total RNA
Precise and Accurate

Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.


Stranded information identifies from which of the 2 DNA strands a given RNA transcript was derived. This information provides increased confidence in transcript annotation, particularly for nonhuman samples. Identifying strand origin increases the percentage of reads that align, reducing sequencing costs per sample.

NeoPrep System enhances TruSeq Stranded mRNA performance

The NeoPrep System enhances TruSeq Stranded mRNA performance by providing high-quality reproducible results, even with low input amounts of RNA. Digital microfluidics technology precisely manipulates droplets that perform the library prep workflow, within the tightly controlled environment of the NeoPrep library card.

A simple, intuitive workflow delivers 16 libraries, eliminating most manual steps, and reducing hands-on time from ~4.5 hours to just 30 minutes. In addition, digital microfluidics requires less RNA input, enabling excellent performance from as little as 25 ng of total RNA.


Method-Specific Workflow Example


Customer Stories

Unraveling Disease Mechanisms With The NextSeq 500 System

Researchers at the Icahn School of Medicine at Mount Sinai are studying mtDNA, piRNA, and TCR repertoire diversity to unlock the secrets of cancer, infertility, and autoimmune disease.

Read More
BRC-Seq Customers Benefit from Streamlined BaseSpace Integrated Workflow

BaseSpace enables tracking of samples, from library prep to data analysis, helping BRC-Seq deliver high-quality sequencing data and additional value to its customers.

Read More

Product Literature

Manuals and Support Information

TruSeq Stranded mRNA Library Prep Kit for NeoPrep Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq Stranded mRNA Library Prep Kit for NeoPrep Support 

Other Kit Configurations

TruSeq Stranded mRNA Library Prep Kits are also available in kit configurations for manual and high-throughput (liquid handling robot-automatable) workflows.

View Other Kit Versions

Related Products

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TruSeq Stranded mRNA Library Prep Kit

Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.

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TruSeq Nano DNA Library Prep Kit for NeoPrep

DNA library preparation for use with the NeoPrep System, providing comprehensive genomic coverage with low DNA input and minimal hands-on time.

This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERCIAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.