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Requires very little RNA: 500 pg+ of rRNA-depleted RNA or poly(A)+ RNA to create diverse sequencing libraries
Fast protocol with limited hands-on time: directional, diverse libraries created in 4 hours
Illumina-compatible: single-read, paired-end and indexed
Flexible study size: 48 unique indexes available separately for multiplexing
Suitable for degraded, intact, or FFPE samples
This kit generates highly directional RNA-Seq libraries from rRNA-depleted or poly(A)+ RNA from any animal, plant, or bacterial species. Ribo-Zero ribosomal RNA (rRNA) removal kits are recommended for use with ScriptSeq v2 to achieve whole-transcriptome, diverse, stranded libraries.
Note: StarScript AMV Reverse Transcriptase, provided in the ScriptSeq kit, is isolated from Avian Myeloblastosis Virus (AMV). The enzyme is carefully purified to minimize AMV nucleic acid contamination.