Nextera Rapid Capture Custom Enrichment Kit

Custom target enrichment kit to rapidly interrogate those portions of the human genome most important to your specific research. Read More...
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Nextera Rapid Capture Custom Enrichment Kit (48 samples)

FC-140-1007

Nextera Rapid Capture Custom Enrichment Kit (96 samples)

FC-140-1008

Nextera Rapid Capture Custom Enrichment Kit (288 samples)

FC-140-1009

Product Highlights

  • Integrated library preparation and enrichment workflow – Nextera tagmentation and optimized hybridization reduce workflow duration, generating data faster
  • Target regions of interest – Choose 0.5–15 Mb of custom content in a single enrichment assay
  • Evolve design with add-on content – Supplement existing panels and keep adding on as your research needs expand

Nextera Rapid Capture Custom Enrichment provides focused access to genomic regions of interest. Leverage this all-in-one library preparation and enrichment workflow, and design your preferred assay with a user-friendly online tool.

This custom assay allows researchers to sequence precious samples quickly and efficiently, using as little as 50 ng of input DNA. The kit offers add-on functionality to refine content over time, or add regions of unique interest to established panels such as Nextera Rapid Capture Exome or TruSight content sets.

Rapidly Interrogate Key Areas of Interest


With Nextera Rapid Capture Custom Enrichment, you can rapidly interrogate those portions of the human genome most important to your project. Densely pack hybridization probes into a region as small as 0.5 Mb, or space them more liberally to enrich up to 15 Mb of fully custom content. Nextera Rapid Capture Custom Enrichment offers maximum flexibility to address specific needs.

Increase Genomic Regions Enriched by the Assay


Add-on functionality allows you to periodically increase those regions of the genome enriched by the custom assay. You can start from our pre-defined panels, such as the Nextera Rapid Capture Exome and add in regions of particular interest, or start from an existing custom design and capitalize on new discoveries. For example, a project can start with existing exome content and add in important non-coding content.

Investigate Automation Options

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this kit

Explore Design Options Before Ordering


DesignStudio software is an intuitive and fully supported online tool for designing custom assays. This free tool is available to anyone with a MyIllumina account. Add targets or regions of interest, optimize probe density, explore other design options, and estimate your project cost, all before committing to a final order.

Learn More About DesignStudio

Start a DesignStudio Project (customer login required)

Specifications

Method-Specific Workflow Example

 

Customer Stories

Multigene Sequencing Panels for Variant Discovery

Franco Taroni, MD, uses broad gene panels and the Nextera Rapid Capture Custom Enrichment Kit to study metabolic and neurological disorders.

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New Method Enables Analysis of Preserved Cancer Specimens with Exome Sequencing

A novel QC approach and the Nextera Rapid Capture Exome Kit enable researchers to sequence degraded DNA from FFPE tumor samples successfully.

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Supporting Data and Figures

High Coverage Uniformity Across Custom 12-Plex Pools
High Coverage Uniformity Across Custom 12-Plex Pools

Nextera Rapid Capture Custom Enrichment provides uniform target enrichment across different custom probe sets and individual samples within a 12-plex pool. A. Coverage uniformity is shown as % of targeted bases represented by >0.2x mean coverage. Error bars show standard deviation of uniformity across the 12 pooled samples for each project. B. Coverage uniformity for each of 12 pooled samples within Project 3 is shown. Mean coverage for this run was 300x, and % of targeted bases that were covered at >60x are shown.

 

Manuals and Support Information

Nextera Rapid Capture Custom Enrichment Kit Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All Nextera Rapid Capture Custom Enrichment Kit Support 

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