|Nextera Rapid Capture Custom Enrichment Kit||Nextera Flex for Enrichment||AmpliSeq for Illumina Custom DNA Panel||AmpliSeq for Illumina On-Demand|
|Content Specifications||Custom genomic content of interest (0.5 - 15 Mb)||Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
|Custom content of interest - up to 5 Mb||Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes|
|Description||A custom research workflow that utilizes custom panels, existing Illumina designed panels or previously ordered custom projects to enable enrichment of targeted genomic content.||A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.||A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy.||A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy.|
|Input Quantity||50 ng DNA||10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).||1–100 ng (10 ng recommended per pool)||1-100 ng DNA|
|Mechanism of Action||Enzymatic fragmentation and hybridization-based enrichment||Bead-bound transposomes and hybrid-capture chemistry||Multiplex PCR||Multiplex PCR|
|Method||Custom Sequencing, Target Enrichment , Target Enrichment, Targeted DNA Sequencing||Custom Sequencing, Exome Sequencing , Target Enrichment , Target Enrichment, Targeted DNA Sequencing||Amplicon Sequencing , Custom Sequencing, Genotyping by Sequencing , Targeted DNA Sequencing||Amplicon Sequencing , Targeted DNA Sequencing|
|Multiplexing||12-plex (pool up to 12 samples per enrichment reaction)||Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.||Up to 96-plex||Up to 96-plex|
|Number of Reactions||48, 96, or 288 samples||Custom workflow: 16 or 96 samples||750 or 3000 reactions||24 or 96 reactions|
|Specialized Sample Types||Not FFPE-Compatible||Blood, FFPE Tissue, Saliva||Blood, FFPE Tissue||Blood, FFPE Tissue|
|Species Category||Human||Human, Other||Any Species, Bovine, Canine, Chicken, Hamster, Human, Maize, Mouse, Ovine, Porcine, Rice, Soybean, Tomato||Human|
Franco Taroni, MD, uses broad gene panels and the Nextera Rapid Capture Custom Enrichment Kit to study metabolic and neurological disorders.Learn More
A novel QC approach and the Nextera Rapid Capture Exome Kit enable researchers to sequence degraded DNA from FFPE tumor samples successfully.Learn More
Nextera Rapid Capture Custom Enrichment provides uniform target enrichment across different custom probe sets and individual samples within a 12-plex pool. A. Coverage uniformity is shown as % of targeted bases represented by >0.2x mean coverage. Error bars show standard deviation of uniformity across the 12 pooled samples for each project. B. Coverage uniformity for each of 12 pooled samples within Project 3 is shown. Mean coverage for this run was 300x, and % of targeted bases that were covered at >60x are shown.
Nextera Flex products provide time-saving solutions for a wide range of sequencing applications. The unique bead-linked transposome chemistry integrates DNA extraction, fragmentation, library preparation, and library normalization steps to deliver the fastest, most flexible workflow in the Illumina library prep portfolio.Learn More