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Nextera DNA Library Preparation Kits provide a fast and easy workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. Samples prepared with Nextera kits are compatible with all Illumina sequencers.
With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input.
See a list of automation vendors with robotic systems compatible with this kit
Our latest library preparation solution provides the fastest, most flexible library prep workflow in the Illumina portfolio. The Nextera DNA Flex Library Prep Kit is ideally suited to a wide range of whole-genome sequencing applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
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Researchers develop novel sequencing methods with the MiSeq and HiSeq Systems to understand the epigenome and its impact on cancer and immune diseases.
Learn MoreKarolinska Institutet researchers use Illumina sequencers to identify novel HPV types associated with non-melanoma skin cancers.
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Nextera chemistry simultaneously fragments and tags DNA in a single step. A simple PCR amplification then appends sequencing adapters and sample indexes to each fragment.
Nextera Library Validation and Cluster Density Optimization
Technical Note | PDF < 1 MB
Nextera Low Plex Pooling Guidelines
Technical Note | PDF < 1 MB
Nextera DNA Library Preparation Kits
Data Sheet | PDF < 1 MB
Nextera DNA Library Prep Kit Documentation
Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.
Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.
